An improved purification of ECF1 and ECF1F0 by using a cytochrome bo‐deficient strain of Escherichia coli facilitates crystallization of these complexes

A novel strategy, which employs a cytochrome bo‐lacking strain (GO104) and a modified isolation procedure provides an effective approach for obtaining much purer preparations of ECF1F0 than described previously, as well as for isolating homogeneous and protein‐chemically pure ECF1. ECF1 obtained in this way could be crystallized by vapor‐diffusion using polyethylene glycol (PEG) as a precipitant in a form suitable for X‐ray diffraction analysis. The crystals belong to the orthorhombic space group P212121, with lattice parameters a=110, b=134, and c=269 Å, and diffract to a resolution of at least 6.4 Å.

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