Anti-inflammatory properties of cerium oxide nanoparticles.

The valence and oxygen defect properties of cerium oxide nanoparticles (nanoceria) suggest that they may act as auto-regenerative free radical scavengers. Overproduction of the free radical nitric oxide (NO) by the enzyme inducible nitric oxide synthase (iNOS) has been implicated as a critical mediator of inflammation. NO is correlated with disease activity and contributes to tissue destruction. The ability of nanoceria to scavenge free radicals, or reactive oxygen species (ROS), and inhibit inflammatory mediator production in J774A.1 murine macrophages is investigated. Cells internalize nanoceria, the treatment is nontoxic, and oxidative stress and pro-inflammatory iNOS protein expression are abated with stimulation. In vivo studies show nanoceria deposition in mouse tissues with no pathogenicity. Taken together, it is suggested that cerium oxide nanoparticles are well tolerated in mice and are incorporated into cellular tissues. Furthermore, nanoceria may have the potential to reduce ROS production in states of inflammation and therefore serve as a novel therapy for chronic inflammation.

[1]  T. Xia,et al.  Toxic Potential of Materials at the Nanolevel , 2006, Science.

[2]  R. Tarnuzzer,et al.  Vacancy engineered ceria nanostructures for protection from radiation-induced cellular damage. , 2005, Nano letters.

[3]  Jimmy C. Yu,et al.  Direct sonochemical preparation of high-surface-area nanoporous ceria and ceria-zirconia solid solutions. , 2003, Journal of colloid and interface science.

[4]  Benjamin Gilbert,et al.  Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. , 2008, ACS nano.

[5]  J. Hahn,et al.  Silver-ion-mediated reactive oxygen species generation affecting bactericidal activity. , 2009, Water research.

[6]  S. Seal,et al.  Nanoceria particles prevent ROI-induced blindness. , 2008, Advances in experimental medicine and biology.

[7]  S. Pickering,et al.  Nanostructured cerium oxide: preparation and properties of weakly-agglomerated powders , 1999 .

[8]  A. Hughes,et al.  The role of hydrogen peroxide in the deposition of cerium-based conversion coatings , 2006 .

[9]  S. C. Parker,et al.  Reduction of NO2 on ceria surfaces. , 2006, The journal of physical chemistry. B.

[10]  Avelino Corma,et al.  Spectroscopic evidence for the supply of reactive oxygen during CO oxidation catalyzed by gold supported on nanocrystalline CeO2. , 2005, Journal of the American Chemical Society.

[11]  Jinhee Choi,et al.  Oxidative stress induced by cerium oxide nanoparticles in cultured BEAS-2B cells. , 2008, Toxicology.

[12]  Z. Marković,et al.  Biomedical potential of the reactive oxygen species generation and quenching by fullerenes (C60). , 2008, Biomaterials.

[13]  Sudipta Seal,et al.  Protein adsorption and cellular uptake of cerium oxide nanoparticles as a function of zeta potential. , 2007, Biomaterials.

[14]  S. C. Parker,et al.  Oxidising CO to CO2 using ceria nanoparticles. , 2005, Physical chemistry chemical physics : PCCP.

[15]  H. Ahsan,et al.  Oxygen free radicals and systemic autoimmunity , 2003, Clinical and experimental immunology.

[16]  S. Mehrotra,et al.  Modulation of autoimmune diseases by nitric oxide , 2000, Immunologic research.

[17]  R. Cabrini,et al.  Biological response of tissues with macrophagic activity to titanium dioxide. , 2008, Journal of biomedical materials research. Part A.

[18]  Ralf Hass,et al.  Cellular responses to reactive oxygen species-induced DNA damage and aging , 2008, Biological chemistry.

[19]  S. Seal,et al.  Rare earth nanoparticles prevent retinal degeneration induced by intracellular peroxides , 2006, Nature nanotechnology.

[20]  L. Rogers,et al.  Cardioprotective effects of cerium oxide nanoparticles in a transgenic murine model of cardiomyopathy. , 2007, Cardiovascular research.

[21]  J. Balsinde,et al.  Oxidative stress and arachidonic acid mobilization. , 2006, Biochimica et biophysica acta.

[22]  R. Locksley,et al.  Tumour necrosis factor α restores granulomas and induces parasite egg-laying in schistosome-infected SCID mice , 1992, Nature.

[23]  A. Tremoulet,et al.  Novel therapies for rheumatoid arthritis , 2006, Expert opinion on investigational drugs.

[24]  Takafumi Yoshida,et al.  Pigment epithelium-derived factor inhibits neointimal hyperplasia after vascular injury by blocking NADPH oxidase-mediated reactive oxygen species generation. , 2007, The American journal of pathology.

[25]  O. Shadyro,et al.  Radiation-induced peroxidation and fragmentation of lipids in a model membrane , 2002, International journal of radiation biology.

[26]  M. Das,et al.  Auto-catalytic ceria nanoparticles offer neuroprotection to adult rat spinal cord neurons. , 2007, Biomaterials.

[27]  P. Calverley,et al.  Pathological networking: a new approach to understanding COPD , 2007, Thorax.

[28]  T. Ohsuna,et al.  Ultraviolet Absorption Spectra of CeO2 Nano-Particles , 1999 .

[29]  D. Sparks,et al.  Nanominerals, Mineral Nanoparticles, and Earth Systems , 2008, Science.

[30]  S. Seal,et al.  Superoxide dismutase mimetic properties exhibited by vacancy engineered ceria nanoparticles. , 2007, Chemical communications.

[31]  Sudipta Seal,et al.  The role of cerium redox state in the SOD mimetic activity of nanoceria. , 2008, Biomaterials.

[32]  B. Rzigalinski.  Nanoparticles and Cell Longevity , 2005, Technology in cancer research & treatment.

[33]  Christian Bogdan,et al.  Nitric oxide and the immune response , 2001, Nature Immunology.