Analytical Method Validation for Quantitative Estimation of Chloropicrin by HPLC Method

A simple, selective, precise and accurate High Performance Liquid Chromatographic method for the analysis of Chloropicrin in its formulations was developed and validated in the present study. The mobile phase consists of a mixture of acetonitrile and water in the proportion 50: 50 (v/v). This was found to give sharp peak of Chloropicrin at a run time of 10 min. HPLC analysis of Chloropicrin was carried out at a wave length of 220 nm with a flow rate of 1.0mL/ min. The linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient 0.999 in the concentration range of 50% to 150%. The linear regression equation was y =2055 x – 109.6. The developed method was employed with a high degree of precision and accuracy for the analysis of Chloropicrin. The method was validated for accuracy, precision, robustness, ruggedness and specificity. The Precision, accuracy, sensitivity, short retention time and composition of the mobile phase indicated that this method is useful for the quantification of Chloropicrin.

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