Analysis of protein interactions on protein arrays by a novel spectral surface plasmon resonance imaging.

We presented a novel surface plasmon resonance (SPR) imaging method for analysis of protein arrays based on a wavelength interrogation-based SPR biosensor. The spectral imaging was performed by the combination of position control and resonance wavelengths calculated from SPR reflectivity spectra. The imaging method was evaluated by analyzing interactions of glutathione S-transferase-fusion proteins with their antibodies. Antigen-antibody interactions were successfully analyzed on glutathione S-transferase-fusion protein arrays by using the spectral imaging method, and the results were confirmed by a parallel analysis using a previously used spectral SPR biosensor based on wavelength interrogation. Specific binding of anti-Rac1 and anti-RhoA to Rac1 and RhoA on the protein arrays was qualitatively and quantitatively analyzed by the spectral SPR imaging. Thus, it was suggested that the novel spectral SPR imaging was a useful tool for the high-throughput analysis of protein-protein interactions on protein arrays.

[1]  C. Mirkin,et al.  Protein Nanoarrays Generated By Dip-Pen Nanolithography , 2002, Science.

[2]  Dong Yonggui,et al.  Micro-array detection system for gene expression products based on surface plasmon resonance imaging , 2003 .

[3]  S. Brueck,et al.  A surface plasmon resonance array biosensor based on spectroscopic imaging. , 2001, Biosensors & bioelectronics.

[4]  Se-Hui Jung,et al.  Analysis of protein interactions on protein arrays by a wavelength interrogation‐based surface plasmon resonance biosensor , 2004, Proteomics.

[5]  S. Nock,et al.  Functional protein microarrays. , 2002, Current opinion in chemical biology.

[6]  R. Corn,et al.  Surface plasmon resonance imaging measurements of ultrathin organic films. , 2003, Annual review of physical chemistry.

[7]  Deepak Uttamchandani,et al.  Optical chemical sensing employing surface plasmon resonance , 1988 .

[8]  T. Kodadek.  Protein microarrays: prospects and problems. , 2001, Chemistry & biology.

[9]  K. Ha,et al.  Phospholipase D Is Not Involved in Rho A-Mediated Activation of Stress Fiber Formation , 1997 .

[10]  S. Yi,et al.  Surface Plasmon Resonance Intensity in Ex Situ Analysis of Protein Arrays Using a Wavelength Interrogation-Based Surface Plasmon Resonance Sensor , 2004 .

[11]  Günter Gauglitz,et al.  Surface plasmon resonance sensors: review , 1999 .

[12]  D. J. Harrison,et al.  Label-free reading of microarray-based immunoassays with surface plasmon resonance imaging. , 2004, Analytical chemistry.

[13]  G. Bokoch,et al.  Assay of Cdc42, Rac, and Rho GTPase activation by affinity methods. , 2002, Methods in enzymology.

[14]  E. Lai,et al.  Surface plasmon resonance-based immunoassays. , 2000, Methods.

[15]  Jong Seol Yuk,et al.  Proteomic applications of surface plasmon resonance biosensors: analysis of protein arrays , 2005, Experimental & Molecular Medicine.

[16]  J. D. Swalen,et al.  Plasmon surface polariton dispersion by direct optical observation , 1980 .

[17]  Kwon-Soo Ha,et al.  Analysis of immunoreactions on protein arrays by using wavelength-interrogation-based surface plasmon resonance sensors , 2004 .

[18]  Wolfgang Knoll,et al.  Surface–plasmon microscopy , 1988, Nature.

[19]  Se-Hui Jung,et al.  Sensitivity of ex situ and in situ spectral surface plasmon resonance sensors in the analysis of protein arrays. , 2005, Biosensors & bioelectronics.

[20]  Anthony G. Frutos,et al.  Peer Reviewed: SPR of Ultrathin Organic Films , 1998 .

[21]  I. Pockrand,et al.  Surface plasma oscillations at silver surfaces with thin transparent and absorbing coatings , 1978 .

[22]  Lena Stigh,et al.  Methods for site controlled coupling to carboxymethyldextran surfaces in surface plasmon resonance sensors , 1995 .

[23]  J. M. McDonnell,et al.  Surface plasmon resonance: towards an understanding of the mechanisms of biological molecular recognition. , 2001, Current opinion in chemical biology.

[24]  B. Liedberg,et al.  Surface plasmon resonance for gas detection and biosensing , 1983 .

[25]  P. Brown,et al.  Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions , 2001, Genome Biology.

[26]  H. Raether.  Surface Plasmons on Smooth and Rough Surfaces and on Gratings , 1988 .

[27]  B. Liedberg,et al.  Principles of biosensing with an extended coupling matrix and surface plasmon resonance , 1993 .

[28]  M. Hersam,et al.  Application of scanning probe microscopy to the characterization and fabrication of hybrid nanomaterials , 2004, Microscopy research and technique.

[29]  Hyeon Seung Lee,et al.  Characterization of surface plasmon resonance wavelength by changes of protein concentration on protein chips , 2003 .

[30]  I Lundström,et al.  Surface plasmon resonance (SPR) analysis of coagulation in whole blood with application in prothrombin time assay. , 1999, Biosensors & bioelectronics.

[31]  R. Corn,et al.  SPR of ultrathin organic films , 1998 .

[32]  M. Snyder,et al.  Protein chip technology. , 2003, Current opinion in chemical biology.

[33]  H. Davies,et al.  Profiling of amyloid beta peptide variants using SELDI Protein Chip arrays. , 1999, BioTechniques.

[34]  L. Brehmer,et al.  Dispersive surface plasmon microscopy for the characterization of ultrathin organic films , 1996 .