Evidence for the involvement of dithiol groups in mitochondrial calcium transport: studies with cadmium.

The effect of cadmium on some functions of mitochondria isolated from kidneys of rat was studied. Addition of cadmium chloride to mitochondria induced stimulation of both State 4 respiratory rate and ATPase activity, which are prevented by the addition of ruthenium red. We also show that cadmium inhibits competitively calcium translocation; this inhibitory effect of cadmium is reverted by the addition of dithiothreitol. From these results, it is proposed that, similarly to Ca2+, cadmium penetrates mitochondria and binds to a membrane dithiol group, which is essential for the translocation of the cation.

[1]  K. Åkerman,et al.  Mitochondrial calcium transport. , 1982, Biochimica et biophysica acta.

[2]  J. Sumner.  A METHOD FOR THE COLORIMETRIC DETERMINATION OF PHOSPHORUS. , 1944, Science.

[3]  A. Shamoo,et al.  The electrophoretic properties of a Ca2+ carrier isolated from calf heart inner mitochondrial membrane. , 1980, The Journal of biological chemistry.

[4]  P. Mitchell,et al.  Lanthanide‐sensitive calcium—monocarboxylate symport in rat liver mitochondria , 1977, FEBS letters.

[5]  W. Cleland,et al.  Stability constants of Mg2+ and Cd2+ complexes of adenine nucleotides and thionucleotides and rate constants for formation and dissociation of MgATP and MgADP. , 1984, Biochemistry.

[6]  M. Wikström,et al.  Safranine as a probe of the mitochondrial membrane potential , 1976, FEBS letters.

[7]  H. Tedeschi,et al.  The transport of cations in mitochondria. , 1981, Biochimica et biophysica acta.

[8]  B. Vallee,et al.  Biochemical effects of mercury, cadmium, and lead. , 1972, Annual review of biochemistry.

[9]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[10]  D. J. Perkins.  Studies on the interaction of zinc, cadmium and mercuric ions with native and chemically modified human serum albumin. , 1961, The Biochemical journal.

[11]  A. Lehninger,et al.  An insoluble Ca2+-binding factor from rat liver mitochondria , 1972 .

[12]  K. Reed,et al.  A kinetic study of mitochondrial calcium transport. , 1975, European journal of biochemistry.

[13]  P. Mitchell,et al.  Electric charge stoicheiometry of calcium translocation in rat liver mitochondria , 1977, FEBS letters.

[14]  J. Puskin,et al.  Evidence for more than one Ca2+ transport mechanism in mitochondria. , 1976, Biochemistry.

[15]  D. Sanadi,et al.  Activation of potassium ion transport in mitochondria by cadmium ion. , 1984, European journal of biochemistry.

[16]  K. Reed,et al.  The inhibition of mitochondrial calcium transport by lanthanides and ruthenium red. , 1974, The Biochemical journal.

[17]  C. Moore,et al.  Specific inhibition of mitochondrial Ca++ transport by ruthenium red. , 1971, Biochemical and biophysical research communications.