Effects of Septoglomus constrictum and Bacillus cereus on the competitive growth of Ageratina adenophora

Beneficial microorganisms play a pivotal role in the invasion process of exotic plants, including arbuscular mycorrhizal fungi (AMF) and Bacillus. However, limited research exists on the synergistic influence of AMF and Bacillus on the competition between both invasive and native plants. In this study, pot cultures of Ageratina adenophora monoculture, Rabdosia amethystoides monoculture, and A. adenophora and R. amethystoides mixture were used to investigate the effects of dominant AMF (Septoglomus constrictum, SC) and Bacillus cereus (BC), and the co-inoculation of BC and SC on the competitive growth of A. adenophora. The results showed that inoculation with BC, SC, and BC + SC significantly increased the biomass of A. adenophora by 14.77, 112.07, and 197.74%, respectively, in the competitive growth between A. adenophora and R. amethystoides. Additionally, inoculation with BC increased the biomass of R. amethystoides by 185.07%, while inoculation with SC or BC + SC decreased R. amethystoides biomass by 37.31 and 59.70% compared to the uninoculated treatment. Inoculation with BC significantly increased the nutrient contents in the rhizosphere soil of both plants and promoted their growth. Inoculation with SC or SC + BC notably increased the nitrogen and phosphorus contents of A. adenophora, therefore enhancing its competitiveness. Compared with single inoculation, dual inoculation with SC and BC increased AMF colonization rate and Bacillus density, indicating that SC and BC can form a synergistic effect to further enhance the growth and competitiveness of A. adenophora. This study reveals the distinct role of S. constrictum and B. cereus during the invasion of A. adenophora, and provide new clues to the underlying mechanisms of interaction between invasive plant, AMF and Bacillus.

[1]  Qiao Li,et al.  The effects of allelochemicals from root exudates of Flaveria bidentis on two Bacillus species , 2022, Frontiers in Plant Science.

[2]  F. Gui,et al.  Effect of arbuscular mycorrhizal fungi on the responses of Ageratina adenophora to Aphis gossypii herbivory , 2022, Frontiers in Plant Science.

[3]  F. Gui,et al.  Rhizospheric Bacillus-Facilitated Effects on the Growth and Competitive Ability of the Invasive Plant Ageratina adenophora , 2022, Frontiers in Plant Science.

[4]  T. Thomas,et al.  Arbuscular Mycorrhizal Fungi Contribute to Phosphorous Uptake and Allocation Strategies of Solidago canadensis in a Phosphorous-Deficient Environment , 2022, Frontiers in Plant Science.

[5]  Q. Li,et al.  Distinct soil microbial communities under Ageratina adenophora invasions. , 2022, Plant biology.

[6]  Wenzhen Lai,et al.  A SPX domain-containing phosphate transporter from Rhizophagus irregularis handles phosphate homeostasis at symbiotic interface of arbuscular mycorrhizas. , 2022, The New phytologist.

[7]  Xiaoping Ma,et al.  An Overview: The Toxicity of Ageratina adenophora on Animals and Its Possible Interventions , 2021, International journal of molecular sciences.

[8]  S. Flory,et al.  Soil microbes alter competition between native and invasive plants , 2021, Journal of Ecology.

[9]  R. Prasanna,et al.  Mycorrhizae Helper Bacteria: Unlocking Their Potential as Bioenhancers of Plant–Arbuscular Mycorrhizal Fungal Associations , 2021, Microbial Ecology.

[10]  Huey-Wen Chuang,et al.  Rhizobacterial Bacillus mycoides functions in stimulating the antioxidant defence system and multiple phytohormone signalling pathways to regulate plant growth and stress tolerance , 2021, Journal of applied microbiology.

[11]  Liu Linshan,et al.  Predicting the potential global distribution of Ageratina adenophora under current and future climate change scenarios , 2021, Ecology and evolution.

[12]  Haiyun Xu,et al.  Arbuscular mycorrhizal fungi enhance the growth of the exotic species Ambrosia artemisiifolia , 2021, Journal of Plant Ecology.

[13]  F. Gui,et al.  Beneficial rhizobacterium provides positive plant–soil feedback effects to Ageratina adenophora , 2021 .

[14]  Yan Wang,et al.  Bacillus promotes invasiveness of exotic Flaveria bidentis by increasing its nitrogen and phosphorus uptake , 2021, Journal of Plant Ecology.

[15]  R. Isticato,et al.  Plant Growth Promotion Function of Bacillus sp. Strains Isolated from Salt-Pan Rhizosphere and Their Biocontrol Potential against Macrophomina phaseolina , 2021, International journal of molecular sciences.

[16]  R. Seviour,et al.  Compositional and functional profiling of the rhizosphere microbiomes of the invasive weed Ageratina adenophora and native plants , 2021, PeerJ.

[17]  Lin Zhang,et al.  Different Arbuscular Mycorrhizal Fungi Cocolonizing on a Single Plant Root System Recruit Distinct Microbiomes , 2020, mSystems.

[18]  Lin Zhang,et al.  Arbuscular mycorrhizal fungi enhance mineralization of organic phosphorus (P) by carrying bacteria along their extraradical hyphae. , 2020, The New phytologist.

[19]  L. Herrera-Estrella,et al.  Functional analysis of the OsNPF4.5 nitrate transporter reveals a conserved mycorrhizal pathway of nitrogen acquisition in plants , 2020, Proceedings of the National Academy of Sciences.

[20]  Haiyun Xu,et al.  Rhizoglomus intraradices and associated Brevibacterium frigoritolerans enhance the competitive growth of Flaveria bidentis , 2020, Plant and Soil.

[21]  Z. Münzbergová,et al.  Plant–soil feedback contributes to predicting plant invasiveness of 68 alien plant species differing in invasive status , 2020 .

[22]  J. Cornelissen,et al.  AM Fungi Alleviate Phosphorus Limitation and Enhance Nutrient Competitiveness of Invasive Plants via Mycorrhizal Networks in Karst Areas , 2020, Frontiers in Ecology and Evolution.

[23]  T. Thomas,et al.  Invasion by the weed Conyza canadensis alters soil nutrient supply and shifts microbiota structure , 2020 .

[24]  Bin Zhao,et al.  The auxin-inducible phosphate transporter AsPT5 mediates phosphate transport and is indispensable for arbuscule formation in Chinese milk vetch at moderately high phosphate supply. , 2020, Environmental microbiology.

[25]  Silvia Pleschka,et al.  Common , 2020, Definitions.

[26]  A. Heintz‐Buschart,et al.  Interactions of nitrogen and phosphorus cycling promote P acquisition and explain synergistic plant growth responses. , 2020, Ecology.

[27]  S. Peng,et al.  Arbuscular mycorrhizal fungi are a double-edged sword in plant invasion controlled by phosphorus concentration. , 2019, The New phytologist.

[28]  A. Saxena,et al.  Bacillus species in soil as a natural resource for plant health and nutrition , 2019, Journal of applied microbiology.

[29]  K. Fang,et al.  Differential effects of plant growth-promoting bacteria on invasive and native plants , 2019, South African Journal of Botany.

[30]  C. Abdelly,et al.  Arbuscular mycorrhizal fungus and rhizobacteria affect the physiology and performance ofSulla coronariaplants subjected to salt stress by mitigation of ionic imbalance , 2019, Journal of Plant Nutrition and Soil Science.

[31]  M. Bahram,et al.  The role of arbuscular mycorrhizal fungi in plant invasion trajectory , 2019, Plant and Soil.

[32]  Xingliang Xu,et al.  Author Correction: Evolutionary history resolves global organization of root functional traits , 2019, Nature.

[33]  P. Jha,et al.  Biology and management of the invasive weed Ageratina adenophora (Asteraceae): current state of knowledge and future research needs , 2019, Weed Research.

[34]  Chun-qiang Wei,et al.  Testing of an integrated regime for effective and sustainable control of invasive Crofton weed (Ageratina adenophora) comprising the use of natural inhibitor species, activated charcoal, and fungicide , 2019, Weed Biology and Management.

[35]  A. Bano,et al.  Comparative Physiological and Metabolic Analysis Reveals a Complex Mechanism Involved in Drought Tolerance in Chickpea (Cicer arietinum L.) Induced by PGPR and PGRs , 2019, Scientific Reports.

[36]  R. Koide,et al.  Common mycorrhizal networks influence the distribution of mineral nutrients between an invasive plant, Solidago canadensis, and a native plant, Kummerowa striata , 2018, Mycorrhiza.

[37]  R. Koide,et al.  Common mycorrhizal networks influence the distribution of mineral nutrients between an invasive plant, Solidago canadensis, and a native plant, Kummerowa striata , 2018, Mycorrhiza.

[38]  Xiaofeng Song,et al.  Bacillus velezensis FZB42 in 2018: The Gram-Positive Model Strain for Plant Growth Promotion and Biocontrol , 2018, Front. Microbiol..

[39]  F. Wan,et al.  AM fungi facilitate the competitive growth of two invasive plant species, Ambrosia artemisiifolia and Bidens pilosa , 2018, Mycorrhiza.

[40]  Lin Zhang,et al.  Signal beyond nutrient, fructose, exuded by an arbuscular mycorrhizal fungus triggers phytate mineralization by a phosphate solubilizing bacterium , 2018, The ISME Journal.

[41]  R. Warren,et al.  Do novel weapons that degrade mycorrhizal mutualisms promote species invasion? , 2018, Plant Ecology.

[42]  Inderjit,et al.  Arbuscular mycorrhizal fungi facilitate growth and competitive ability of an exotic species Flaveria bidentis , 2017 .

[43]  Qisheng Feng,et al.  A New Strategy for the Prevention and Control of Eupatorium adenophorum under Climate Change in China , 2017 .

[44]  M. Mack,et al.  Ecosystem vs. community recovery 25 years after grass invasions and fire in a subtropical woodland , 2017 .

[45]  Martin A. Nuñez,et al.  The emerging science of linked plant-fungal invasions. , 2017, The New phytologist.

[46]  J. Klironomos,et al.  Does responsiveness to arbuscular mycorrhizal fungi depend on plant invasive status? , 2017, Ecology and evolution.

[47]  Calum Brown,et al.  Snow damage to the canopy facilitates alien weed invasion in a subtropical montane primary forest in southwestern China , 2017 .

[48]  J. Maron,et al.  Reduced mycorrhizal responsiveness leads to increased competitive tolerance in an invasive exotic plant , 2016 .

[49]  T. Sa,et al.  Arbuscular mycorrhizal fungi and associated bacteria isolated from salt-affected soil enhances the tolerance of maize to salinity in coastal reclamation soil , 2016 .

[50]  W. Dawson,et al.  Identifying the role of soil microbes in plant invasions , 2016 .

[51]  Y. Lekberg,et al.  Do native and invasive plants differ in their interactions with arbuscular mycorrhizal fungi? A meta‐analysis , 2015 .

[52]  Karen C. Abbott,et al.  Spatial Heterogeneity in Soil Microbes Alters Outcomes of Plant Competition , 2015, PloS one.

[53]  G. Gupta,et al.  Plant Growth Promoting Rhizobacteria (PGPR): Current and Future Prospects for Development of Sustainable Agriculture , 2015 .

[54]  Jia-kuan Chen,et al.  Spatial distribution of bacterial communities driven by multiple environmental factors in a beach wetland of the largest freshwater lake in China , 2015, Front. Microbiol..

[55]  Fusuo Zhang,et al.  Hyphosphere interactions between an arbuscular mycorrhizal fungus and a phosphate solubilizing bacterium promote phytate mineralization in soil , 2014 .

[56]  L. Comas,et al.  Root traits contributing to plant productivity under drought , 2013, Front. Plant Sci..

[57]  W. H. van der Putten,et al.  Soil biotic legacy effects of extreme weather events influence plant invasiveness , 2013, Proceedings of the National Academy of Sciences.

[58]  S. Gibbons,et al.  Severe plant invasions can increase mycorrhizal fungal abundance and diversity , 2013, The ISME Journal.

[59]  Jonathan M. Chase,et al.  Invasive Plants Have Scale-Dependent Effects on Diversity by Altering Species-Area Relationships , 2013, Science.

[60]  D. Richardson,et al.  Naturalization of introduced plants: ecological drivers of biogeographical patterns. , 2012, The New phytologist.

[61]  R. Freckleton,et al.  Unraveling the Life History of Successful Invaders , 2012, Science.

[62]  Liang Mei,et al.  Changes in non-symbiotic nitrogen-fixing bacteria inhabiting rhizosphere soils of an invasive plant Ageratina adenophora. , 2012 .

[63]  Sally E. Smith,et al.  Roles of arbuscular mycorrhizas in plant nutrition and growth: new paradigms from cellular to ecosystem scales. , 2011, Annual review of plant biology.

[64]  F. Wan,et al.  Determination of the population genetic structure of the invasive weed Ageratina adenophora using ISSR-PCR markers , 2009, Russian Journal of Plant Physiology.

[65]  J. Bever,et al.  Mycorrhizal densities decline in association with nonnative plants and contribute to plant invasion. , 2009, Ecology.

[66]  Wan-xue Liu,et al.  An invasive aster (Ageratina adenophora) invades and dominates forest understories in China: altered soil microbial communities facilitate the invader and inhibit natives , 2007, Plant and Soil.

[67]  Rui Wang,et al.  Invasion dynamics and potential spread of the invasive alien plant species Ageratina adenophora (Asteraceae) in China , 2006 .

[68]  J. Connolly,et al.  Designs for greenhouse studies of interactions between plants , 1999 .

[69]  Manuela Giovannetti,et al.  AN EVALUATION OF TECHNIQUES FOR MEASURING VESICULAR ARBUSCULAR MYCORRHIZAL INFECTION IN ROOTS , 1980 .

[70]  OUP accepted manuscript , 2021, Tree Physiology.

[71]  Rout George Kerry,et al.  Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture. , 2018, Microbiological research.

[72]  M. Lara,et al.  Plant-symbiont interactions: the functional role of expansins , 2017, Symbiosis.

[73]  D. Du,et al.  Soil microbiota and plant invasions: current and future , 2017 .

[74]  J. Weremijewicz,et al.  Arbuscular common mycorrhizal networks mediate intra- and interspecific interactions of two prairie grasses , 2017, Mycorrhiza.

[75]  Sicuia Oana Alina,et al.  Biodiversity of Bacillus subtilis group and beneficial traits of Bacillus species useful in plant protection , 2015 .

[76]  Zhang Jinlin,et al.  Effects of Flaveria bidentis invasion on soil microbial communities, enzyme activities and nutrients , 2012 .

[77]  于文清,et al.  外来植物紫茎泽兰入侵对土壤理化性质及丛枝菌根真菌(AMF)群落的影响 , 2012 .

[78]  Li Bao-ping.  Effects of fertilization on the relative competitive ability of Eupatorium adenophorum and Lolium perenne at their seedling stage , 2007 .

[79]  L. Oksanen,et al.  On the indices of plant–plant competition and their pitfalls , 2006 .

[80]  S. Payne,et al.  Detection, isolation, and characterization of siderophores. , 1994, Methods in enzymology.

[81]  R. Isaac,et al.  High speed analysis of agricultural samples using inductively coupled plasma-atomic emission spectroscopy , 1983 .

[82]  B. Biermann,et al.  QUANTIFYING VESICULAR-ARBUSCULAR MYCORRHIZAE: A PROPOSED METHOD TOWARDS STANDARDIZATION * , 1981 .

[83]  D. W. Nelson,et al.  Determination of Total Nitrogen in Plant Material1 , 1973 .