Tubular gel fabrication and cell encapsulation in laminar flow stream formed by microfabricated nozzle array.

We have used a laminar flow stream formed by a microfabricated nozzle array to prepare cell-encapsulated alginate gel micro-tubes, in which cells formed a cylindrical multi-cellular aggregate after cultivation for two weeks.

[1]  Shinji Sakai,et al.  Development of mammalian cell-enclosing calcium-alginate hydrogel fibers in a co-flowing stream. , 2006, Biotechnology journal.

[2]  Minoru Seki,et al.  Interfacial Tension Driven Monodispersed Droplet Formation from Microfabricated Channel Array , 2001 .

[3]  Toshiro Higuchi,et al.  Droplet formation in a microchannel network. , 2002, Lab on a chip.

[4]  P. Chang,et al.  Osmotic pressure test: a simple, quantitative method to assess the mechanical stability of alginate microcapsules. , 2001, Journal of biomedical materials research.

[5]  Keng-Shiang Huang,et al.  Manipulating the generation of Ca-alginate microspheres using microfluidic channels as a carrier of gold nanoparticles. , 2006, Lab on a chip.

[6]  S. Quake,et al.  Dynamic pattern formation in a vesicle-generating microfluidic device. , 2001, Physical review letters.

[7]  H. Mizumoto,et al.  Formation of cylindrical multicellular aggregate (cylindroid) and expression of liver specific functions of primary rat hepatocytes , 1999, Cytotechnology.

[8]  Y. Rhee,et al.  Generation of monodisperse alginate microbeads and in situ encapsulation of cell in microfluidic device , 2007, Biomedical microdevices.

[9]  G. Whitesides,et al.  Generation of monodisperse particles by using microfluidics: control over size, shape, and composition. , 2005, Angewandte Chemie.

[10]  S. Sugiura,et al.  Size control of calcium alginate beads containing living cells using micro-nozzle array. , 2005, Biomaterials.

[11]  S. Takeuchi,et al.  Monodisperse Alginate Hydrogel Microbeads for Cell Encapsulation , 2007 .

[12]  H. Fujita,et al.  Silicon array of elongated through-holes for monodisperse emulsion droplets , 2002 .

[13]  S. Sugiura,et al.  Microfabricated airflow nozzle for microencapsulation of living cells into 150 micrometer microcapsules , 2007, Biomedical microdevices.

[14]  Su-Jung Shin,et al.  "On the fly" continuous generation of alginate fibers using a microfluidic device. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[15]  Shinji Sakai,et al.  Fabrication of Artificial Endothelialized Tubes with Predetermined Three‐Dimensional Configuration from Flexible Cell‐Enclosing Alginate Fibers , 2007, Biotechnology progress.

[16]  Ethan Tumarkin,et al.  Microfluidic production of biopolymer microcapsules with controlled morphology. , 2006, Journal of the American Chemical Society.

[17]  E. Kumacheva,et al.  Continuous microfluidic reactors for polymer particles. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[18]  H. Stone,et al.  Formation of dispersions using “flow focusing” in microchannels , 2003 .

[19]  H Yang,et al.  Comparative studies of in vitro and in vivo function of three different shaped bioartificial pancreases made of agarose hydrogel. , 1994, Biomaterials.

[20]  D. Weitz,et al.  Monodisperse Double Emulsions Generated from a Microcapillary Device , 2005, Science.

[21]  Robert Langer,et al.  Endothelialized microvasculature based on a biodegradable elastomer. , 2005, Tissue engineering.