Loss of maternal alleles on chromosome arm 11p in hepatoblastoma.

Hepatoblastoma is the most common primary malignant liver tumor in children, yet little is known about molecular genetic changes in these tumors. Previous studies report loss of heterozygosity on chromosome arm 11p in some hepatoblastomas. We used the polymerase chain reaction to amplify multiple microsatellites on chromosome arm 11p to assess loss of heterozygosity in 18 hepatoblastomas. Loss of heterozygosity on 11p was found in six of them. The common region of overlap was restricted to the telomeric portion of 11p (11p15.5) and therefore excluded the WT-1 tumor suppressor gene at 11p13. Parental origin of the lost allele could be determined in all six cases and was exclusively maternal. These results indicate that a tumor suppressor gene at 11p15.5 is involved in the pathogenesis of hepatoblastoma and also suggest that this chromosomal region is imprinted.

[1]  A. von Deimling,et al.  A device for processing large acrylamide gels. , 1994, BioTechniques.

[2]  A. Feinberg,et al.  Transcribed dinucleotide repeat polymorphism in the IGF2 gene. , 1994, Human Molecular Genetics.

[3]  O. Wiestler,et al.  Microsatellite analysis of loss of heterozygosity on chromosomes 9q, 11 p and 17p in medulloblastomas , 1994, Neuropathology and applied neurobiology.

[4]  I. Leuschner,et al.  Clinico-pathological criteria with prognostic relevance in hepatoblastoma. , 1994, European journal of cancer.

[5]  K. Tadokoro,et al.  PCR detection of 9 polymorphisms in the WT1 gene. , 1993, Human molecular genetics.

[6]  S. Davies,et al.  Maintenance of genomic imprinting at the IGF2 locus in hepatoblastoma. , 1993, Cancer research.

[7]  L. Simms,et al.  Three non‐overlapping regions of chromosome arm 11p allele loss identified in infantile tumors of adrenal and liver , 1993, Genes, chromosomes & cancer.

[8]  E. Mariman,et al.  The insulin–like growth factor type–2 receptor gene is imprinted in the mouse but not in humans , 1993, Nature Genetics.

[9]  A. Feinberg Genomic imprinting and gene activation in cancer , 1993, Nature Genetics.

[10]  D. Collier,et al.  Dopamine D4 receptor subtypes and response to clozapine , 1993, The Lancet.

[11]  M. Litt,et al.  Dinucleotide repeat polymorphisms at the D11S439 and HBB loci. , 1992, Human molecular genetics.

[12]  E. Rogaev,et al.  Dinucleotide repeat polymorphisms at the P1, HBE1 and MYH7 loci. , 1992, Human molecular genetics.

[13]  M. Litt,et al.  Microsatellite polymorphisms at the D11S554 and D11S569 loci. , 1992, Human molecular genetics.

[14]  F. Giardiello,et al.  Risk of hepatoblastoma in familial adenomatous polyposis. , 1991, The Journal of pediatrics.

[15]  D. S. Rath,et al.  Tetranucleotide repeat polymorphism at the human tyrosine hydroxylase gene (TH). , 1991, Nucleic acids research.

[16]  P. Hoban,et al.  HinfI polymorphism within the 3' untranslated region of the candidate Wilms tumour gene. , 1991, Nucleic acids research.

[17]  C. Junien,et al.  Report of the committee on chromosome and gene loss in human neoplasia , 1991 .

[18]  M. Litt,et al.  Dinucleotide repeat polymorphism at the D11S490 locus. , 1990, Nucleic acids research.

[19]  N. Kasthuri,et al.  Malignant hepatic tumours in children: incidence, clinical features and aetiology. , 1990, Paediatric and perinatal epidemiology.

[20]  R. Whitehead,et al.  Autocrine regulation of human tumor cell proliferation by insulin-like growth factor II: an in-vitro model. , 1990, Endocrinology.

[21]  G. Kovacs,et al.  Cytogenetic and molecular studies on six sporadic hepatoblastomas , 1989 .

[22]  N. Copeland,et al.  Loss of heterozygosity in three embryonal tumours suggests a common pathogenetic mechanism , 1985, Nature.

[23]  K. Ishak Primary hepatic tumors in childhood. , 1976, Progress in liver diseases.