论文信息 - Continous hydrogen production by immobilized whole cells of Clostridium butyricum

Continous hydrogen production by immobilized whole cells of Clostridium butyricum

Clostridium butyricum IFO 3847 was immobilized in polyacrylamide gel. Increase of the polymer concentration decreased hydrogen evolution from glucose by whole cells. The immobilized cells continuously evolved hydrogen from glucose under aerobic conditions. The pH stability of hydrogen production increased with immobilization, and the optimum temperature was 37°C. No difference in the amount of evolved hydrogen was observed after storage for one month at 5°C.

[1] R. Thauer,et al. Properties and function of the pyruvate-formate-lyase reaction in clostridiae. , 1972, European journal of biochemistry.

[2] H. Gest,et al. Biological Formation of Molecular Hydrogen , 1965, Science.

[3] Shuichi Suzuki,et al. Immobilized Enzyme Technology , 1975 .

[4] G. Hicks,et al. The preparation and characterization of lyophilized polyacrylamide enzyme gels for chemical analysis. , 1966, Analytical chemistry.

[5] Samuel Raeburn,et al. Pyruvate: ferredoxin oxidoreductase. II. Characteristics of the forward and reverse reactions and properties of the enzyme. , 1971, Archives of biochemistry and biophysics.

[6] J. Rabinowitz,et al. Pyruvate: ferredoxin oxidoreductase. I. The pyruvate-CO 2 exchange reaction. , 1971, Archives of biochemistry and biophysics.

[7] T. Hattori,et al. CHEMICAL ACTIVITIES OF E. COLI ADSORBED ON A RESIN , 1960 .

[8] T. Hattori,et al. Chemical activities of Azotobacter agile adsorbed on a resin. , 1961, Journal of biochemistry.