Interferon‐γ inhibits expression of the long pentraxin PTX3 in human monocytes

PTX3 is a prototypic long pentraxin expressed by various cell types, most prominently monocytes and endothelial cells, in response to interleukin‐1 (IL‐1), tumor necrosis factor (TNF) and bacterial products. In the present report, we show that interferon‐γ (IFN‐γ) inhibits the expression of the PTX3 gene induced by exposure to IL‐1, TNF or lipopolysaccharide in human monocytes. This effect is dose dependent and observable when IFN‐γ is added from 24 h before up to 3 h after the addition of IL‐1. While the time course of the IL‐1‐induced PTX3 mRNA expression is not affected, IFN‐γ reduces the stability of the PTX3 mRNA as well as its transcription. The inhibition of PTX3 expression is restricted to monocytes in that no inhibition occurs in cytokine‐stimulated fibroblasts and endothelial cells. Under the same conditions, as expected, IFN‐γ augmented monocyte chemotactic protein‐1 expression in the same cell preparations. PTX3 protein secretion by activated monocytes is also suppressed by exposure to IFN‐γ. Altogether, these data identify a negative pathway of regulation mediated by IFN‐γ, which may occur under inflammatory conditions.

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