Three-Dimensional Map of Descemet Membrane Endothelial Keratoplasty Detachment

Abstract Purpose To develop, validate, and apply a neural network for quantification of endothelial corneal graft detachment using anterior-segment optical coherence tomography (AS-OCT). Design Training and validation of a neural network and application within a prospective cohort. Participants Patients two weeks after Descemet membrane endothelial keratoplasty. Methods Investigators manually labeled the posterior cornea and the graft in cross-sectional images of rotational AS-OCT scans in the training and validation set. Neural networks for image segmentation were trained to identify the area of graft detachment on cross-sectional images. The best-performing neural network with the lowest misclassification (Youden index) and highest spatial overlap with the ground truth (Dice coefficient) was selected and evaluated in a separate dataset. Three-dimensional maps of the area and volume of graft detachment were calculated. For application, the neural network’s rating on the percent detachment was compared with slit-lamp-based ratings of cornea specialists on the same day as the AS-OCT took place. Main Outcome Measures Youden index and Dice coefficient. Results Neural networks were trained on 27 AS-OCT scans with 6912 labeled images. Among 48 combinations of probability thresholds and epoch states, the best-performing neural network had a Youden index of 0.99 with a Dice coefficient of 0.78, indicating low misclassification and good spatial overlap on individual image segmentation. In the validation set unknown to the neural network with 20 scans (5120 images), the Youden index was 0.85 with a Dice coefficient of 0.73, and a high overall performance compared to the manually-labeled ground truth (R2 = 0.90). In the application set with 107 eyes, the neural network estimated the mean percent detachment larger than the cornea specialist (mean difference, 8.2 percentage points; 95% CI, 6.2–10.2). Masked review of 42 AS-OCTs with more than ±10 percentage points difference in ratings showed that clinician underestimated the true detachment in cases with significant detachment requiring intervention. Conclusions Deep learning-based segmentation of AS-OCT images quantified the percent and the volume of DMEK graft detachment with high precision. Fully automated three-dimensional quantification of graft detachment is highly sensitive, particularly in corneas with a significant amount of detachment, and may support decision making.