X-ray-Induced Shortwave Infrared Biomedical Imaging Using Rare-Earth Nanoprobes

Shortwave infrared (SWIR or NIR-II) light provides significant advantages for imaging biological structures due to reduced autofluorescence and photon scattering. Here, we report on the development of rare-earth nanoprobes that exhibit SWIR luminescence following X-ray irradiation. We demonstrate the ability of X-ray-induced SWIR luminescence (X-IR) to monitor biodistribution and map lymphatic drainage. Our results indicate X-IR imaging is a promising new modality for preclinical applications and has potential for dual-modality molecular disease imaging.

[1]  Shuo Diao,et al.  Through-skull fluorescence imaging of the brain in a new near-infrared window , 2014, Nature Photonics.

[2]  Anders Tingberg,et al.  Breast tomosynthesis and digital mammography: a comparison of breast cancer visibility and BIRADS classification in a population of cancers with subtle mammographic findings , 2008, European Radiology.

[3]  Dominik J Naczynski,et al.  Rare Earth Nanoprobes for Functional Biomolecular Imaging and Theranostics. , 2014, Journal of materials chemistry. B.

[4]  Ravi Murthy,et al.  Yttrium-90 microsphere therapy for hepatic malignancy: devices, indications, technical considerations, and potential complications. , 2005, Radiographics : a review publication of the Radiological Society of North America, Inc.

[5]  Kevin Welsher,et al.  Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared window , 2011, Proceedings of the National Academy of Sciences.

[6]  S Schaller,et al.  Subsecond multi-slice computed tomography: basics and applications. , 1999, European journal of radiology.

[7]  Ashok Kumar,et al.  Radioprotection of Swiss albino mice by plant extract Mentha piperita (Linn.). , 2003, Journal of radiation research.

[8]  Lei Xing,et al.  Synergistic Assembly of Heavy Metal Clusters and Luminescent Organic Bridging Ligands in Metal–Organic Frameworks for Highly Efficient X-ray Scintillation , 2014, Journal of the American Chemical Society.

[9]  T. Julian,et al.  Sentinel-lymph-node resection compared with conventional axillary-lymph-node dissection in clinically node-negative patients with breast cancer: overall survival findings from the NSABP B-32 randomised phase 3 trial. , 2010, The Lancet. Oncology.

[10]  Lei Xing,et al.  Tomographic molecular imaging of x-ray-excitable nanoparticles. , 2010, Optics letters.

[11]  Ian M. Kennedy,et al.  NaGdF4:Eu3+ Nanoparticles for Enhanced X-ray Excited Optical Imaging , 2014, Chemistry of materials : a publication of the American Chemical Society.

[12]  L Xing,et al.  Motion correction for improved target localization with on-board cone-beam computed tomography , 2006, Physics in medicine and biology.

[13]  A. García-Adeva,et al.  Infrared-to-visible upconversion of Er 3+ ions in GeO 2 –PbO–Nb 2 O 5 glasses , 2004 .

[14]  O. Urakawa,et al.  Small - , 2007 .

[15]  Didier Gourier,et al.  Nanoprobes with near-infrared persistent luminescence for in vivo imaging , 2007, Proceedings of the National Academy of Sciences.

[16]  Kai Yang,et al.  Biodistribution, pharmacokinetics and toxicology of Ag2S near-infrared quantum dots in mice. , 2013, Biomaterials.

[17]  W. Miller,et al.  TLD assessment of mouse dosimetry during microCT imaging. , 2008, Medical physics.

[18]  M. C. Mancini,et al.  Bioimaging: second window for in vivo imaging. , 2009, Nature nanotechnology.

[19]  Dianna D Cody,et al.  In vivo respiratory-gated micro-CT imaging in small-animal oncology models. , 2004, Molecular imaging.

[20]  Nicole M. Iverson,et al.  In Vivo Biosensing Via Tissue Localizable Near Infrared Fluorescent Single Walled Carbon Nanotubes , 2013, Nature nanotechnology.

[21]  B. Wall,et al.  Rare-earth-doped biological composites as in vivo shortwave infrared reporters , 2013, Nature Communications.

[22]  Fei Wang,et al.  Sub-10 nm BaYF5:Yb3+,Er3+ core–shell nanoparticles with intense 1.53 μm fluorescence for polymer-based waveguide amplifiers , 2013 .

[23]  Jianhua Hao,et al.  Dual-modal upconversion fluorescent/X-ray imaging using ligand-free hexagonal phase NaLuF4:Gd/Yb/Er nanorods for blood vessel visualization. , 2014, Biomaterials.

[24]  Lei Xing,et al.  X-Ray Luminescence Computed Tomography via Selective Excitation: A Feasibility Study , 2010, IEEE Transactions on Medical Imaging.

[25]  Guosong Hong,et al.  Multifunctional in vivo vascular imaging using near-infrared II fluorescence , 2012, Nature Medicine.

[26]  Daniel Bell.  RadioGraphics , 2017, Radiopaedia.org.

[27]  J. Boone,et al.  Small-animal X-ray dose from micro-CT. , 2004, Molecular imaging.

[28]  Lei Xing,et al.  Synthesis and Radioluminescence of PEGylated Eu3+‐doped Nanophosphors as Bioimaging Probes , 2011, Advanced materials.

[29]  Jaetae Lee,et al.  In Vivo Imaging of Sentinel Nodes Using Fluorescent Silica Nanoparticles in Living Mice , 2010, Molecular Imaging and Biology.

[30]  Kai Yang,et al.  Highly-sensitive multiplexed in vivo imaging using pegylated upconversion nanoparticles , 2010 .

[31]  Rui Hu,et al.  A pilot study in non-human primates shows no adverse response to intravenous injection of quantum dots. , 2012, Nature nanotechnology.

[32]  J. Frangioni.  In vivo near-infrared fluorescence imaging. , 2003, Current opinion in chemical biology.

[33]  B. Hasegawa,et al.  Radiation dose estimate in small animal SPECT and PET. , 2004, Medical physics.

[34]  T. MacVittie,et al.  Postirradiation treatment with granulocyte colony-stimulating factor and preirradiation WR-2721 administration synergize to enhance hemopoietic reconstitution and increase survival. , 1992, International journal of radiation oncology, biology, physics.

[35]  Michael S Strano,et al.  M13 phage-functionalized single-walled carbon nanotubes as nanoprobes for second near-infrared window fluorescence imaging of targeted tumors. , 2012, Nano letters.

[36]  Yang Yang,et al.  Long-term in vivo biodistribution imaging and toxicity of polyacrylic acid-coated upconversion nanophosphors. , 2010, Biomaterials.

[37]  A. Rogach,et al.  Infrared-emitting colloidal nanocrystals: synthesis, assembly, spectroscopy, and applications. , 2007, Small.

[38]  E. Radzhabov,et al.  Luminescence of BaF2–LaF3 , 2001 .

[39]  Zhuang Liu,et al.  A route to brightly fluorescent carbon nanotubes for near-infrared imaging in mice. , 2009, Nature nanotechnology.

[40]  M. Tan,et al.  Optical Efficiency of Short Wave Infrared Emitting Phosphors , 2011 .

[41]  G. Chow,et al.  Synthesis of Hexagonal‐Phase NaYF4:Yb,Er and NaYF4:Yb,Tm Nanocrystals with Efficient Up‐Conversion Fluorescence , 2006 .

[42]  D. S. Bradshaw,et al.  Photonics , 2023, 2023 International Conference on Electrical Engineering and Photonics (EExPolytech).

[43]  Lei Xing,et al.  Hard X-ray-induced optical luminescence via biomolecule-directed metal clusters. , 2014, Chemical communications.

[44]  M. Bawendi,et al.  Selection of Quantum Dot Wavelengths for Biomedical Assays and Imaging , 2003, Molecular Imaging.

[45]  Dominik J Naczynski,et al.  Albumin nanoshell encapsulation of near-infrared-excitable rare-Earth nanoparticles enhances biocompatibility and enables targeted cell imaging. , 2010, Small.

[46]  宁北芳,et al.  疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A , 2005 .

[47]  Heping Zeng,et al.  Optical gain at 1550 nm from colloidal solution of Er3+-Yb3+ codoped NaYF4 nanocubes. , 2009, Optics express.

[48]  Julia F. Barrett,et al.  Artifacts in CT: recognition and avoidance. , 2004, Radiographics : a review publication of the Radiological Society of North America, Inc.

[49]  W. Lu,et al.  Synergistic dual-modality in vivo upconversion luminescence/X-ray imaging and tracking of amine-functionalized NaYbF(4):Er nanoprobes. , 2014, ACS applied materials & interfaces.

[50]  Yong Zhang,et al.  Biocompatibility of silica coated NaYF(4) upconversion fluorescent nanocrystals. , 2008, Biomaterials.

[51]  L Xing,et al.  Hybrid x-ray/optical luminescence imaging: characterization of experimental conditions. , 2010, Medical physics.