Effect of long-term 50 Hz magnetic field exposure on the micronucleated polychromatic erythrocytes of mice

Abstract In recent years extremely low-frequency magnetic fields (ELF-EMF) have become widely used in human activities, leading to an increased chance of exposure to ELF-EMF. There are few reports on in vivo mammalian genotoxic effects using micronucleus (MN) assays, which generally have been used as a short-term screening system. We analyzed the possible genotoxic effect induced by long-term exposure (7, 14, 21, 28 d) of a 50 Hz ELM-MF to mice by measuring the increase in frequency of micronucleated polychromatic erythrocyte in their bone marrow (MNPCEs) and we compared it with that induced by 50 cGy of X-rays. Subsequently, we tried to reduce this chromosomal damage by administering four antioxidants substances with radioprotective capacities: dimethyl sulfoxide (DMSO), 6-n-propyl-2-thiouracil (PTU), grape-procyanidins (P) and citrus flavonoids extract (CE). The increase in micronucleated cells was higher in both physical treatments (Control < ELF-EMF (p < 0.01)  0.001)); however, the antioxidant substances only showed a genoprotective capacity against the damage induced by ionizing radiation (Ci > PTU = DMSO (p < 0.001) >P = CE (p < 0.001). The 50 Hz ELM-MF increased MNPCEs in mouse bone marrow, expressing a genotoxic capacity. Administration of antioxidant substances with radioprotective capacities known to act through the elimination of free radicals did not diminish the genotoxic effect induced by ELM-MF.

[1]  L. Verschaeve,et al.  Effect of coexposure to 50 Hz magnetic fields and an aneugen on human lymphocytes, determined by the cytokinesis block micronucleus assay , 2003, Bioelectromagnetics.

[2]  S. Monarca,et al.  Genotoxic effects of extremely low frequency (ELF) magnetic fields (MF) evaluated by the Tradescantia‐micronucleus assay , 2005, Environmental toxicology.

[3]  J. A. Gabaldón,et al.  Rosmarinic acid, a photo-protective agent against UV and other ionizing radiations. , 2009, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[4]  Ayla Celik,et al.  Cytogenetic effects of extremely low frequency magnetic field on Wistar rat bone marrow. , 2007, Mutation research.

[5]  Nicola Vanello,et al.  Is the genotoxic effect of magnetic resonance negligible? Low persistence of micronucleus frequency in lymphocytes of individuals after cardiac scan. , 2008, Mutation research.

[6]  M. Kirsch‐Volders,et al.  The in vitro MN assay in 2011: origin and fate, biological significance, protocols, high throughput methodologies and toxicological relevance , 2011, Archives of Toxicology.

[7]  D. Weiss,et al.  Micronucleus induction in Syrian hamster embryo cells following exposure to 50 Hz magnetic fields, benzo(a)pyrene, and TPA in vitro. , 2001, Mutation research.

[8]  Myeong Seong Kim,et al.  Genotoxic effects of 3 T magnetic resonance imaging in cultured human lymphocytes , 2011, Bioelectromagnetics.

[9]  Micronuclei in the blood and bone marrow cells of mice exposed to specific complex time‐varying pulsed magnetic fields , 2010, Bioelectromagnetics.

[10]  Michael Fenech,et al.  The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure. , 2011, Mutagenesis.

[11]  M C Cimino,et al.  The in vivo micronucleus assay in mammalian bone marrow and peripheral blood. A report of the U.S. Environmental Protection Agency Gene-Tox Program. , 1990, Mutation research.

[12]  M. Alcaraz,et al.  Radioprotective effects in vivo of phenolics extracted from Olea europaea L. leaves against X-ray-induced chromosomal damage: comparative study versus several flavonoids and sulfur-containing compounds. , 2002, Journal of medicinal food.

[13]  J. Heddle,et al.  Chromosomal aberrations and bone marrow toxicity. , 1981, Environmental health perspectives.

[14]  A. Dudarewicz,et al.  Static magnetic field affects oxidative stress in mouse cochlea. , 2010, International journal of occupational medicine and environmental health.

[15]  J. D. del Río,et al.  Antioxidant activity and radioprotective effects against chromosomal damage induced in vivo by X-rays of flavan-3-ols (Procyanidins) from grape seeds (Vitis vinifera): comparative study versus other phenolic and organic compounds. , 2000, Journal of agricultural and food chemistry.

[16]  V. Vicente,et al.  Biological dosimetry and Bayesian analysis of chromosomal damage in thyroid cancer patients. , 2007, Radiation protection dosimetry.

[17]  V. Ortega,et al.  Ausencia de daño cromosómico y genotóxico inducido por la dosis de radiación administrada en las exploraciones gammagráficas , 2004 .

[18]  Vijayalaxmi,et al.  Controversial cytogenetic observations in mammalian somatic cells exposed to extremely low frequency electromagnetic radiation: A review and future research recommendations , 2005, Bioelectromagnetics.

[19]  J. Odum,et al.  Lack of activity of estradiol in rodent bone marrow micronucleus assays. , 1997, Mutation research.

[20]  A. Vral,et al.  Cytokinesis-blocked micronucleus assay and cancer risk assessment. , 2011, Mutagenesis.

[21]  G. Gajski,et al.  Assessment of cytogenetic damage and oxidative stress in personnel occupationally exposed to the pulsed microwave radiation of marine radar equipment. , 2011, International journal of hygiene and environmental health.

[22]  L. Mazur.  Induction of micronucleated erythrocytes by MEA, AET, WR-2721 and X-rays. , 1995, Mutation research.

[23]  C. Tanzarella,et al.  Clastogenicity and aneuploidy in newborn and adult mice exposed to 50 Hz magnetic fields , 2006, International journal of radiation biology.

[24]  J. Hur,et al.  Optimal Spray Time, Interval and Number of Preventive Fungicides for the Control of Fruit Rots of Green and Gold Kiwifruit Cultivars , 2013 .

[25]  Sevil Zencir,et al.  Oxidative Stress and Apoptosis in Relation to Exposure to Magnetic Field , 2011, Cell Biochemistry and Biophysics.

[26]  M. Alcaraz,et al.  Chemical genoprotection: reducing biological damage to as low as reasonably achievable levels. , 2011, Dento maxillo facial radiology.

[27]  Satnam Singh,et al.  Mutagenic Potential of Benzo(A)Pyrene and N-Nitrosodiethylamine is not Affected by 50-Hz Sinusoidal Electromagnetic Field , 1997 .

[28]  W Schmid,et al.  The micronucleus test. , 1975, Mutation research.

[29]  I. Çelik,et al.  Effects of long-term 50 Hz magnetic field exposure on the micro nucleated polychromatic erythrocyte and blood lymphocyte frequency and argyrophilic nucleolar organizer regions in lymphocytes of mice. , 2010, Neuro endocrinology letters.

[30]  J. T. Macgregor,et al.  Micronucleus assays in rodent tissues other than bone marrow. , 2011, Mutagenesis.

[31]  D. Weiss,et al.  Alteration in cellular functions in mouse macrophages after exposure to 50 Hz magnetic fields , 2006, Journal of cellular biochemistry.

[32]  G. Köteles,et al.  The present state and perspectives of micronucleus assay in radiation protection. A review. , 1987, International journal of radiation applications and instrumentation. Part A, Applied radiation and isotopes.

[33]  Radioprotective Effects Against Chromosomal Damage Induced in Human Lymphocytes by gamma-Rays as a Function of Polymerization Grade of Grape Seed Extracts. , 2001, Journal of medicinal food.

[34]  Hye Kyoung Jeon,et al.  Effects of Extremely Low-Frequency Electromagnetic Fields on Delayed Chromosomal Instability Induced by Bleomycin in Normal Human Fibroblast Cells , 2007, Journal of toxicology and environmental health. Part A.

[35]  J. Miyakoshi,et al.  Effects of exposure of CHO-K1 cells to a 10-T static magnetic field. , 2002, Radiology.

[36]  C. Acevedo,et al.  Liposoluble antioxidants provide an effective radioprotective barrier. , 2009, The British journal of radiology.

[37]  M Simkó,et al.  Micronucleus formation in human amnion cells after exposure to 50 Hz MF applied horizontally and vertically. , 1998, Mutation research.

[38]  L. Abramsson-Zetterberg,et al.  Extended exposure of adult and fetal mice to 50 Hz magnetic field does not increase the incidence of micronuclei in erythrocytes , 2001, Bioelectromagnetics.

[39]  R. Kavet,et al.  Can low-level 50/60 Hz electric and magnetic fields cause biological effects? , 1997, Radiation research.

[40]  H. Chung,et al.  The effect of extremely low frequency electromagnetic fields (ELF-EMF) on the frequency of micronuclei and sister chromatid exchange in human lymphocytes induced by benzo(a)pyrene. , 2003, Toxicology letters.

[41]  R. Pasquini,et al.  Micronucleus induction in cells co-exposed in vitro to 50 Hz magnetic field and benzene, 1,4-benzenediol (hydroquinone) or 1,2,4-benzenetriol. , 2003, Toxicology in vitro : an international journal published in association with BIBRA.

[42]  Robert Winker,et al.  Chromosomal damage in human diploid fibroblasts by intermittent exposure to extremely low-frequency electromagnetic fields. , 2005, Mutation research.

[43]  Hideyuki Okano,et al.  Effects of static magnetic fields in biology: role of free radicals. , 2008, Frontiers in bioscience : a journal and virtual library.

[44]  C. Acevedo,et al.  Radioprotective-antimutagenic effects of rosemary phenolics against chromosomal damage induced in human lymphocytes by gamma-rays. , 2006, Journal of agricultural and food chemistry.

[45]  L. Sarma,et al.  Protective Effects of Vitamins C and E Against γ-ray-induced Chromosomal Damage in Mouse , 1993 .

[46]  M. Alcaraz,et al.  Antioxidant and Radioprotective Effects of Olive Leaf Extract , 2010 .