DC-SIGN ( CD 209 ) Mediates Dengue Virus Infection of Human Dendritic Cells

Dengue virus is a single-stranded, enveloped RNA virus that productively infects human dendritic cells (DCs) primarily at the immature stage of their differentiation. We now find that all four serotypes of dengue use DC-SIGN (CD209), a C-type lectin, to infect dendritic cells. THP-1 cells become susceptible to dengue infection after transfection of DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), or its homologue L-SIGN, whereas the infection of dendritic cells is blocked by anti–DC-SIGN antibodies and not by antibodies to other molecules on these cells. Viruses produced by dendritic cells are infectious for DC-SIGN– and L-SIGN– bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection.

[1]  B. Gicquel,et al.  The Cell Surface Receptor DC-SIGN Discriminates betweenMycobacterium Species through Selective Recognition of the Mannose Caps on Lipoarabinomannan* , 2003, The Journal of Biological Chemistry.

[2]  R. Steinman,et al.  Cell type-dependent retention and transmission of HIV-1 by DC-SIGN. , 2003, International immunology.

[3]  O. Schwartz,et al.  DC-SIGN Is the Major Mycobacterium tuberculosis Receptor on Human Dendritic Cells , 2003, The Journal of experimental medicine.

[4]  T. Geijtenbeek,et al.  Mycobacteria Target DC-SIGN to Suppress Dendritic Cell Function , 2003, The Journal of experimental medicine.

[5]  R. Steinman,et al.  Preparation of clinical-grade recombinant canarypox-human immunodeficiency virus vaccine-loaded human dendritic cells. , 2002, The Journal of infectious diseases.

[6]  R. Doms,et al.  Quantitative Expression and Virus Transmission Analysis of DC-SIGN on Monocyte-Derived Dendritic Cells , 2002, Journal of Virology.

[7]  B. Berkhout,et al.  Differential Transmission of Human Immunodeficiency Virus Type 1 by Distinct Subsets of Effector Dendritic Cells , 2002, Journal of Virology.

[8]  Á. Corbí,et al.  C-Type Lectins DC-SIGN and L-SIGN Mediate Cellular Entry by Ebola Virus in cis and in trans , 2002, Journal of Virology.

[9]  Philip K. Russell,et al.  Hemorrhagic fever viruses as biological weapons: medical and public health management. , 2002, JAMA.

[10]  D. Gubler.  Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century. , 2002, Trends in microbiology.

[11]  M. Malim,et al.  cis Expression of DC-SIGN Allows for More Efficient Entry of Human and Simian Immunodeficiency Viruses via CD4 and a Coreceptor , 2001, Journal of Virology.

[12]  J. Mascola,et al.  Human dendritic cells as targets of dengue virus infection. , 2001, The journal of investigative dermatology. Symposium proceedings.

[13]  F. Ennis,et al.  Human Dendritic Cells Are Activated by Dengue Virus Infection: Enhancement by Gamma Interferon and Implications for Disease Pathogenesis , 2001, Journal of Virology.

[14]  M. Carrington,et al.  A Dendritic Cell–Specific Intercellular Adhesion Molecule 3–Grabbing Nonintegrin (Dc-Sign)–Related Protein Is Highly Expressed on Human Liver Sinusoidal Endothelial Cells and Promotes HIV-1 Infection , 2001, The Journal of experimental medicine.

[15]  R. Doms,et al.  DC-SIGNR, a DC-SIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[16]  D. Chang,et al.  Infection of Human Dendritic Cells by Dengue Virus Causes Cell Maturation and Cytokine Production1 , 2001, The Journal of Immunology.

[17]  Carl G. Figdor,et al.  DC-SIGN–ICAM-2 interaction mediates dendritic cell trafficking , 2000, Nature Immunology.

[18]  J. Mascola,et al.  Human skin Langerhans cells are targets of dengue virus infection , 2000, Nature Medicine.

[19]  C. Figdor,et al.  Identification of DC-SIGN, a Novel Dendritic Cell–Specific ICAM-3 Receptor that Supports Primary Immune Responses , 2000, Cell.

[20]  Douglas S Kwon,et al.  DC-SIGN, a Dendritic Cell–Specific HIV-1-Binding Protein that Enhances trans-Infection of T Cells , 2000, Cell.

[21]  C. King,et al.  Analysis of the steps involved in Dengue virus entry into host cells. , 1999, Virology.

[22]  C. King,et al.  Bacterial Lipopolysaccharide Inhibits Dengue Virus Infection of Primary Human Monocytes/Macrophages by Blockade of Virus Entry via a CD14-Dependent Mechanism , 1999, Journal of Virology.

[23]  G. Schuler,et al.  Generation of large numbers of fully mature and stable dendritic cells from leukapheresis products for clinical application. , 1999, Journal of immunological methods.

[24]  J. Esko,et al.  Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate , 1997, Nature Medicine.

[25]  D. Morens,et al.  Dengue 1 virus binding to human hepatoma HepG2 and simian Vero cell surfaces differs. , 1996, The Journal of general virology.

[26]  A. Nisalak,et al.  Antibody-dependent enhancement of dengue virus growth in human monocytes as a risk factor for dengue hemorrhagic fever. , 1989, The American journal of tropical medicine and hygiene.

[27]  A. Allison,et al.  Dengue viruses and mononuclear phagocytes. II. Identity of blood and tissue leukocytes supporting in vitro infection , 1977, The Journal of experimental medicine.

[28]  S. Halstead,et al.  Antibody-enhanced dengue virus infection in primate leukocytes , 1977, Nature.

[29]  W. Brandt,et al.  Isolation of a temperature-sensitive dengue-2 virus under conditions suitable for vaccine development , 1976, Infection and immunity.

[30]  A. Tokunaga,et al.  A Summary of , 2011 .

[31]  Bachti Alisjahbana,et al.  Epidemiology of dengue and dengue hemorrhagic fever in a cohort of adults living in Bandung, West Java, Indonesia. , 2005, The American journal of tropical medicine and hygiene.

[32]  W. Hendrickson,et al.  DC-SIGN-mediated internalization of HIV is required for trans-enhancement of T cell infection. , 2002, Immunity.

[33]  M. Colmenares.  Dendritic-cell specific ICAM-3 grabbing nonintegrin (DC-SIGN, CD209), a C-type surface lectin in human dendritic cells, is a receptor for Leishmania amastigotes , 2002 .

[34]  Dengue and dengue haemorrhagic fever. 1990-1994. , 1995, Releve epidemiologique hebdomadaire.

[35]  A. Nisalak,et al.  A prospective study of dengue infections in Bangkok. , 1988, The American journal of tropical medicine and hygiene.