Rapid identification of saponins in plant extracts by electrospray ionization multi-stage tandem mass spectrometry and liquid chromatography/tandem mass spectrometry.

Electrospray ionization multi-stage tandem mass spectrometry (ESI-MS(n)) and liquid chromatography coupled with on-line mass spectrometry (LC/MS/MS) were applied to characterize saponins in crude extracts from Panax ginseng. The MS(n) data of the [M - H](-) ions of saponins can provide structural information on the sugar sequences of the saccharide chains and on the sapogins of saponins. By ESI-MS(n), non-isomeric saponins and isomeric saponins with different aglycones can be determined rapidly in plant extracts. LC/MS/MS is a good complementary analytical tool for determination of isomeric saponins. These approaches constitute powerful analytical tools for rapid screening and structural assignment of saponins in plant extracts.

[1]  S. Takahashi,et al.  The preventive effect of ginseng with du-zhong leaf on protein metabolism in aging. , 1997, Biological & pharmaceutical bulletin.

[2]  C. Jang,et al.  Antinarcotic effects of the standardized ginseng extract G115 on morphine. , 1990, Planta medica.

[3]  C. Kensil,et al.  Saponin adjuvant induction of ovalbumin-specific CD8+ cytotoxic T lymphocyte responses. , 1992, Journal of immunology.

[4]  G. Fenwick,et al.  Saponin composition of 13 varieties of legume seed using fast atom bombardment mass spectrometry , 1988 .

[5]  Xian-guo He,et al.  Analysis of flavonoids from red clover by liquid chromatography—electrospray mass spectrometry , 1996 .

[6]  M. Maillard,et al.  Determination of saponins in crude plant extracts by liquid chromatography—thermospray mass spectrometry , 1993 .

[7]  V. Wray,et al.  Application of MS-MS for the rapid, comparative analysis of saponin mixtures as exemplified by the deacylated and partially deacylated triterpenoid saponins of Bellis annua. , 1996, Planta medica.

[8]  C. Kensil,et al.  Saponin adjuvant enhancement of antigen-specific immune responses to an experimental HIV-1 vaccine. , 1992, Journal of immunology.

[9]  M. Careri,et al.  Overview of the applications of liquid chromatography–mass spectrometry interfacing systems in food analysis: naturally occurring substances in food , 1998 .

[10]  G. Moneti,et al.  Mass spectrometric characterization of flavonoids in extracts from Passiflora incarnata , 1997 .

[11]  J. Tabet,et al.  Fast atom bombardment mass spectrometry of triterpenic saponins , 1986 .