论文信息 - A Method for Screening for Hydrogenase Negative Mutants of Azotobacter chroococcum

A Method for Screening for Hydrogenase Negative Mutants of Azotobacter chroococcum

SUMMARY: Slow reduction of methylene blue under H2, after treatment with NaF + EDTA, distinguished Hup− (uptake hydrogenase negative) from Hup+ colonies of Azotobacter chroococcum on plates.

F. B. Simpson | J. Postgate | M. Yates | R. Robson | C. Partridge

[1] H. Evans,et al. Revertible hydrogen uptake-deficient mutants of Rhizobium japonicum , 1981, Journal of bacteriology.

[2] S. L. Albrecht,et al. Hydrogen metabolism in the legume-Rhizobium symbiosis. , 1980 .

[3] J. Postgate,et al. Rhizobium japonicum mutants unable to use hydrogen , 1978, Nature.

[4] F. B. Simpson,et al. Expression of hydrogenase activity in free-living Rhizobium japonicum. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[5] J. Postgate,et al. A note on the effect of diluents on the determination of viable numbers of Azotobacteriaceae. , 1970, The Journal of applied bacteriology.

[6] P. W. Wilson,et al. Direct demonstration of ammonia as an intermediate in nitrogen fixation by Azotobacter. , 1953, The Journal of biological chemistry.