A yeast two-hybrid smart-pool-array system for protein-interaction mapping

We present here a new two-hybrid smart pool array (SPA) system in which, instead of individual activation domain strains, well-designed activation domain pools are screened in an array format that allows built-in replication and prey-bait deconvolution. Using this method, a Saccharomyces cerevisiae genome SPA increases yeast two-hybrid screening efficiency by an order of magnitude.

[1]  Gary D Bader,et al.  Analyzing yeast protein–protein interaction data obtained from different sources , 2002, Nature Biotechnology.

[2]  M. Vidal,et al.  Interactome: gateway into systems biology. , 2005, Human molecular genetics.

[3]  Sean R. Collins,et al.  Global landscape of protein complexes in the yeast Saccharomyces cerevisiae , 2006, Nature.

[4]  R. Rosenfeld.  Nature , 2009, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[5]  S. Schreiber,et al.  A yeast genetic system for selecting small molecule inhibitors of protein-protein interactions in nanodroplets. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[6]  BMC Bioinformatics , 2005 .

[7]  Gerard Tromp,et al.  A strategy for constructing large protein interaction maps using the yeast two-hybrid system: regulated expression arrays and two-phase mating. , 2003, Genome research.

[8]  Brian E Snydsman,et al.  Assigning function to yeast proteins by integration of technologies. , 2003, Molecular cell.

[9]  James R. Knight,et al.  A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae , 2000, Nature.

[10]  A. Barabasi,et al.  Network biology: understanding the cell's functional organization , 2004, Nature Reviews Genetics.

[11]  S. Fields,et al.  A pooling-deconvolution strategy for biological network elucidation , 2006, Nature Methods.

[12]  S. Fields,et al.  Protein analysis on a proteomic scale , 2003, Nature.

[13]  D. Eisenberg,et al.  Detecting protein function and protein-protein interactions from genome sequences. , 1999, Science.

[14]  A. Grigoriev.  On the number of protein-protein interactions in the yeast proteome. , 2003, Nucleic acids research.

[15]  Nicolas Thierry-Mieg,et al.  A new pooling strategy for high-throughput screening: the Shifted Transversal Design , 2006, BMC Bioinformatics.

[16]  Mark Gerstein,et al.  Predicting interactions in protein networks by completing defective cliques , 2006, Bioinform..

[17]  김삼묘,et al.  “Bioinformatics” 특집을 내면서 , 2000 .

[18]  Barry Schweitzer,et al.  Microarrays to characterize protein interactions on a whole‐proteome scale , 2003, Proteomics.