Sweet pepper seed quality and lea-protein activity in relation to fruit maturation and post-harvest storage.

This study was conducted to evaluate the infl uence of fruit maturity and post-harvest storage on physiological quality and LEA proteins activity of sweet pepper (Capsicum annuum L.) seeds. Fruits were harvested at 40, 50, 60 and 70 days after anthesis (DAA) and stored for 0, 3, 6, 9, 12 and 15 days before seed extraction. Seeds were evaluated by germination, fi rst count, seedling length, accelerated aging, speed emergence index, electrical conductivity and eletrophoretical analysis for proteins LEA. It was verifi ed that precocious harvest (40 DAA) was not benefi cial to physiological quality of pepper seeds even when associated to 15 days post-harvest storage. For fruits harvested at 50 DAA, 12 days post-harvest storage was indispensable to assure the physiological quality of seeds. Pepper seeds harvested from 60 DAA presented high quality, not being necessary the postharvest storage of fruits. LEA proteins synthesis occurred at 60 DAA, being directly related to physiological quality of pepper seeds.

[1]  É. R. V. V. Pinho,et al.  GERMINABILIDADE E TOLERÂNCIA À DESSECAÇÃO EM SEMENTES DE MILHO COLHIDAS EM DIFERENTES ESTÁDIOS DE MATURAÇÃO , 2010 .

[2]  D. Dias,et al.  Teste de envelhecimento acelerado em sementes de pimenta , 2006 .

[3]  L. Dias,et al.  Tomato seed quality in relation to fruit maturation and post-harvest storage , 2006 .

[4]  S. D. V. F. Rosa,et al.  Enzimas removedoras de radicais livres e proteínas lea associadas à tolerância de sementes milho à alta temperatura de secagem , 2005 .

[5]  R. Bino,et al.  Gene Expression Programs during Brassica oleracea Seed Maturation, Osmopriming, and Germination Are Indicators of Progression of the Germination Process and the Stress Tolerance Level1 , 2005, Plant Physiology.

[6]  R. Quatrano,et al.  Common amino acid sequence domains among the LEA proteins of higher plants , 1989, Plant Molecular Biology.

[7]  L. Dure,et al.  Sequence and characterization of 6 Lea proteins and their genes from cotton , 1988, Plant Molecular Biology.

[8]  D. M. Tekrony,et al.  Handbook Of Vigour Test Methods , 2004 .

[9]  K. Chourey,et al.  Accumulation of LEA proteins in salt (NaCl) stressed young seedlings of rice (Oryza sativa L.) cultivar Bura Rata and their degradation during recovery from salinity stress. , 2003, Journal of plant physiology.

[10]  E. Golovina,et al.  Mechanisms of plant desiccation tolerance. , 2001, Trends in plant science.

[11]  I. Demir,et al.  Seed quality in relation to fruit maturation and seed dry weight during development in tomato , 2001 .

[12]  C. Job,et al.  Sugarbeet seed priming: effects of priming conditions on germination, solubilization of 11-S globulin and accumulation of LEA proteins , 2000, Seed Science Research.

[13]  J. Nakagawa,et al.  Efeito da idade e do repouso pós-colheita de frutos de pepino na semente armazenada , 1999 .

[14]  A. Kermode Approaches to elucidate the basis of desiccation-tolerance in seeds , 1997, Seed Science Research.

[15]  B. B. Desai,et al.  Seeds handbook: biology, production, processing, and storage. , 1997 .

[16]  F. J. Sundstrom,et al.  Fruit maturity, storage and postharvest maturation treatments affect bell pepper (Capsicum annuum L.) seed quality , 1993 .

[17]  I. Demir,et al.  Development of pepper (Capsicum annuum) seed quality , 1992 .

[18]  I. Demir,et al.  Changes in seed quality during seed development and maturation in tomato , 1992, Seed Science Research.

[19]  A. Leopold,et al.  Maturation proteins associated with desiccation tolerance in soybean. , 1991, Plant physiology.

[20]  D. W. Hughes,et al.  Accumulation kinetics of cotton late embryogenesis-abundant mRNAs and storage protein mRNAs: coordinate regulation during embryogenesis and the role of abscisic acid. , 1987, Developmental biology.

[21]  F. J. Sundstrom,et al.  Afterripening and Harvesting Effects on Tabasco Pepper Seed Germination Performance , 1987, HortScience.

[22]  K. Thompson,et al.  Seeds: Physiology of Development and Germination , 1986 .