Erythrocytes (RBC) of adult Japanese quail, Coturnix coturnix japonica, were found to be as sensitive as day-old chick RBC for rubella virus hemagglutination (HA). Various factors involved in the HA were studied with quail RBC as well as with adult pigeon and day-old chick RBC. Pigeon RBC, unlike chick and quail RBC, tended to show, without hemagglutinin, a pattern of sedimented RBC resembling the HA pattern by the virus at 4 C, to a lesser extent at room temperature, in 0.85% NaCl solution buffered with m/100 phosphate (PBS), and were prevented from doing so by addition of a small amount of bovine plasma albumin to the diluent. A small amount (about 10−3 m) of CaCL2 in PBS gave higher HA titers. The HA titer was higher at 4 C than at room temperature and much reduced at 36 C with chick and quail RBC. With pigeon RBC, addition of bovine plasma albumin to the diluent tended to reduce HA titers. The HA titer was highest at pH 5.8 to 6.8, and was inversely proportional to the RBC concentration. Under the conditions established on the basis of these findings, chick and quail RBC gave similar HA titers, but pigeon RBC gave consistently higher titers. However, these types of RBC gave no significant difference in HA-inhibiting antibody titer when 4 units of hemagglutinin as determined with the homologous RBC was used.
[1]
頼夫 日沼,et al.
風疹ウイルスの赤血球凝集反応 (HA) ならびに同抑制反応 (HAI) の術式に関する研究
,
1968
.
[2]
M. F. Warburton,et al.
Is rubella an arbovirus?
,
1967,
Lancet.
[3]
S. Plotkin,et al.
Studies on Hemagglutination by Rubella Virus.∗
,
1967
.
[4]
J. Peetermans,et al.
[The use of pigeon eruthrocytes in the hemagglutination inhibition test for rubella].
,
1967,
La Presse medicale.
[5]
J. Stewart,et al.
Rubella Hemagglutinin Prepared with Alkaline Extraction of Virus Grown in Suspension Culture of BHK-21 Cells
,
1967,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.
[6]
R. D. Douglas,et al.
Rubella-virus hemagglutination-inhibition test.
,
1967,
The New England journal of medicine.
[7]
J. Sever.
Application of a microtechnique to viral serological investigations.
,
1962,
Journal of immunology.