Validation of HPLC method for the simultaneous and quantitative determination of 12 UV‐filters in cosmetics

The aim of the study was the validation of a high‐performance liquid chromatography (HPLC) method for the simultaneous and quantitative determination of twelve commonly used organic UV‐filters (phenylbenzimidazole sulfonic acid, benzophenone‐3, isoamyl p‐methoxycinnamate, diethylamino hydroxybenzoyl hexyl benzoate, octocrylene, ethylhexyl methoxycinnamate, ethylhexyl salicylate, butyl methoxydibenzoylmethane, diethylhexyl butamido triazone, ethylhexyl triazone, methylene bis‐benzotriazolyl tetramethylbutylphenol and bis‐ethylhexyloxyphenol methoxyphenyl triazine) contained in suncare products. The separation and quantitative determination was performed in <30 min, using a Symmetry Shield® C18 (5 μm) column from Waters and a mobile phase (gradient mode) consisting of ethanol and acidified water. UV measurements were carried out at multi‐wavelengths, according to the absorption of the analytes.

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