Improving antibody affinity by mimicking somatic hypermutation in vitro

[1]  I. Pastan,et al.  Improved stability and yield of a Fv-toxin fusion protein by computer design and protein engineering of the Fv. , 1998, Journal of molecular biology.

[2]  R. Gelber,et al.  New treatments for breast cancer: breakthroughs for patient care or just steps in the right direction? , 1998, Annals of oncology : official journal of the European Society for Medical Oncology.

[3]  A. Pini,et al.  Design and Use of a Phage Display Library , 1998, The Journal of Biological Chemistry.

[4]  L. Norton,et al.  Recombinant humanized anti-HER2 antibody (Herceptin) enhances the antitumor activity of paclitaxel and doxorubicin against HER2/neu overexpressing human breast cancer xenografts. , 1998, Cancer research.

[5]  M. Hensler,et al.  Stepwise in vitro affinity maturation of Vitaxin, an αvβ3-specific humanized mAb , 1998 .

[6]  G. Adams,et al.  Increased affinity leads to improved selective tumor delivery of single-chain Fv antibodies. , 1998, Cancer research.

[7]  I. Pastan,et al.  Isolation of a high-affinity stable single-chain Fv specific for mesothelin from DNA-immunized mice by phage display and construction of a recombinant immunotoxin with anti-tumor activity. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[8]  D. Maloney,et al.  IDEC-C2B8 (Rituximab) Anti-CD20 Monoclonal Antibody Therapy in Patients With Relapsed Low-Grade Non-Hodgkin's Lymphoma , 1997 .

[9]  M. Dyer,et al.  Phase II multicenter study of human CD52 antibody in previously treated chronic lymphocytic leukemia. European Study Group of CAMPATH-1H Treatment in Chronic Lymphocytic Leukemia. , 1997, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[10]  D. Maloney,et al.  IDEC-C 2 B 8 ( Rituximab ) Anti-CD 20 Monoclonal Antibody Therapy in Patients With Relapsed Low-Grade Non-Hodgkin ’ s Lymphoma , 1997 .

[11]  C. Milstein,et al.  Modifying the sequence of an immunoglobulin V-gene alters the resulting pattern of hypermutation. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[12]  I. Pastan,et al.  Engineering antibody Fv fragments for cancer detection and therapy: Bisulfide-stabilized Fv fragments , 1996, Nature Biotechnology.

[13]  G. Winter,et al.  Mimicking somatic hypermutation: affinity maturation of antibodies displayed on bacteriophage using a bacterial mutator strain. , 1996, Journal of molecular biology.

[14]  N A Kolchanov,et al.  Somatic hypermutagenesis in immunoglobulin genes. III. Somatic mutations in the chicken light chain locus. , 1996, Biochimica et biophysica acta.

[15]  I. Pastan,et al.  Treatment of advanced solid tumors with immunotoxin LMB–1: An antibody linked to Pseudomonas exotoxin , 1996, Nature Medicine.

[16]  J. Bye,et al.  Isolation of high-affinity monomeric human anti-c-erbB-2 single chain Fv using affinity-driven selection. , 1996, Journal of molecular biology.

[17]  I. Pastan,et al.  Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[18]  T. Waldmann,et al.  Radioimmunotherapy of interleukin-2R alpha-expressing adult T-cell leukemia with Yttrium-90-labeled anti-Tac. , 1995, Blood.

[19]  D R Burton,et al.  CDR walking mutagenesis for the affinity maturation of a potent human anti-HIV-1 antibody into the picomolar range. , 1995, Journal of molecular biology.

[20]  C. Milstein,et al.  Codon bias targets mutation , 1995, Nature.

[21]  J. Bajorath,et al.  Affinity maturation of the BR96 anti-carcinoma antibody by codon-based mutagenesis. , 1995, Journal of immunology.

[22]  T. Logtenberg,et al.  Selection and application of human single chain Fv antibody fragments from a semi-synthetic phage antibody display library with designed CDR3 regions. , 1995, Journal of molecular biology.

[23]  C. Milstein,et al.  Somatic mutation of immunoglobulin lambda chains: a segment of the major intron hypermutates as much as the complementarity-determining regions. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[24]  R. Insel,et al.  Bias in somatic hypermutation of human VH genes. , 1994, International immunology.

[25]  L L Houston,et al.  Highly specific in vivo tumor targeting by monovalent and divalent forms of 741F8 anti-c-erbB-2 single-chain Fv. , 1993, Cancer research.

[26]  G R Pilkington,et al.  Molecular profile of an antibody response to HIV-1 as probed by combinatorial libraries. , 1993, Journal of molecular biology.

[27]  J. Bye,et al.  Human anti‐self antibodies with high specificity from phage display libraries. , 1993, The EMBO journal.

[28]  H. Gadner,et al.  A Controlled Trial of Recombinant Human Granulocyte-macrophage Colony-stimulating Factor After Total-body Irradiation, High-dose Chemotherapy, and Autologous Bone-marrow Transplantation for Acute Lymphoblastic-leukemia Or Malignant-lymphoma , 1992 .

[29]  N A Kolchanov,et al.  Somatic hypermutagenesis in immunoglobulin genes. II. Influence of neighbouring base sequences on mutagenesis. , 1992, Biochimica et biophysica acta.

[30]  T. Yokota,et al.  Rapid tumor penetration of a single-chain Fv and comparison with other immunoglobulin forms. , 1992, Cancer research.

[31]  C. Barbas,et al.  In vitro selection and affinity maturation of antibodies from a naive combinatorial immunoglobulin library. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[32]  I. Pastan,et al.  B3(Fv)-PE38KDEL, a single-chain immunotoxin that causes complete regression of a human carcinoma in mice. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[33]  N. Kolchanov,et al.  Somatic hypermutagenesis in immunoglobulin genes. I. Correlation between somatic mutations and repeats. Somatic mutation properties and clonal selection. , 1991, Biochimica et biophysica acta.

[34]  R. Jain,et al.  Interstitial transport of rabbit and sheep antibodies in normal and neoplastic tissues. , 1990, Cancer research.

[35]  R K Jain,et al.  Physiological barriers to delivery of monoclonal antibodies and other macromolecules in tumors. , 1990, Cancer research.

[36]  T. Waldmann,et al.  A recombinant immunotoxin consisting of two antibody variable domains fused to Pseudomonas exotoxin , 1989, Nature.

[37]  V. V. Solovyov,et al.  Pecularities of immunoglobulin gene structures as a basis for somatic mutation emergence , 1987, FEBS letters.

[38]  G. B. Golding,et al.  Patterns of somatic mutations in immunoglobulin variable genes. , 1987, Genetics.

[39]  Thomas A. Kunkel,et al.  Rapid and efficient site-specific mutagenesis without phenotypic selection. , 1985, Proceedings of the National Academy of Sciences of the United States of America.