On-site quantitative Hg2+ measurements based on selective and sensitive fluorescence biosensor and miniaturized smartphone fluorescence microscope.

Mercury is a bio-accumulative and toxic pollutant causing severe damages to human health and environment. Since Hg2+ is the most stable form of mercury, selective and sensitive Hg2+ detection is required. Though classical approaches can realize accurate Hg2+ detection, the complicated instruments and the time-consuming operations inevitably limit their on-site applications. Here, we design a smart Hg2+ detection approach using the fluorescence biosensor, the smartphone fluorescence microscope and the smartphone application for Hg2+ on-site detection. Based on the thymine-Hg2+-thymine coordination chemistry, a selective and sensitive fluorescence biosensor is designed for capturing Hg2+ in aqueous solution; besides, a miniaturized smartphone fluorescence microscope for fluorescence signal collection and an image processing application for quantitative Hg2+ measurements are constructed. A highly specific detection of Hg2+ with a linear relation between 1 nM and 1 μM with a limit of detection of 1 nM is obtained using the smart Hg2+ detection approach. Considering it can realize selective and sensitive quantitative Hg2+ measurements in high precision with simple operations and cost-effective system, it is believed the proposed smart Hg2+ detection approach owns great potentials in Hg2+ detection for routine uses at home and in the field.

[1]  Derek K. Tseng,et al.  Detection and Spatial Mapping of Mercury Contamination in Water Samples Using a Smart-Phone , 2014, ACS nano.

[2]  C. Zheng,et al.  Advanced oxidation using Fe(3)O(4) magnetic nanoparticles and its application in mercury speciation analysis by high performance liquid chromatography-cold vapor generation atomic fluorescence spectrometry. , 2013, The Analyst.

[3]  Chih-Ching Huang,et al.  Gold nanoparticle probes for the detection of mercury, lead and copper ions. , 2011, The Analyst.

[4]  Itamar Willner,et al.  Optical analysis of Hg2+ ions by oligonucleotide-gold-nanoparticle hybrids and DNA-based machines. , 2008, Angewandte Chemie.

[5]  Ethynyl and π-stacked thymine-Hg2+-thymine base pairs enhanced fluorescence quenching via photoinduced electron transfer and simple and sensitive mercury ion sensing. , 2015, Biosensors & bioelectronics.

[6]  A. Ono,et al.  Highly selective oligonucleotide-based sensor for mercury(II) in aqueous solutions. , 2004, Angewandte Chemie.

[7]  T. Carell,et al.  DNA--metal base pairs. , 2007, Angewandte Chemie.

[8]  Kyuwon Kim,et al.  An electrochemical detection of Hg2 + ion using graphene oxide as an electrochemically active indicator , 2012 .

[9]  Chojiro Kojima,et al.  15N-15N J-coupling across Hg(II): direct observation of Hg(II)-mediated T-T base pairs in a DNA duplex. , 2007, Journal of the American Chemical Society.

[10]  Xingyu Jiang,et al.  Gold nanoparticles for the colorimetric and fluorescent detection of ions and small organic molecules. , 2011, Nanoscale.

[11]  Elizabeth M. Nolan,et al.  Tools and tactics for the optical detection of mercuric ion. , 2008, Chemical reviews.

[12]  T. Baughman.  Elemental Mercury Spills , 2005, Environmental health perspectives.

[13]  T. Kazi,et al.  Determination of total mercury in chicken feed, its translocation to different tissues of chicken and their manure using cold vapour atomic absorption spectrometer. , 2010, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[14]  Aydogan Ozcan,et al.  A personalized food allergen testing platform on a cellphone. , 2013, Lab on a chip.

[15]  David Erickson,et al.  A smartphone platform for the quantification of vitamin D levels. , 2014, Lab on a chip.

[16]  D W Boening,et al.  Ecological effects, transport, and fate of mercury: a general review. , 2000, Chemosphere.

[17]  D. Xing,et al.  Rapid and highly sensitive detection of mercury ion (Hg2+) by magnetic beads-based electrochemiluminescence assay. , 2010, Biosensors & bioelectronics.

[18]  Cesar M. Castro,et al.  Integrated Magneto-Chemical Sensor For On-Site Food Allergen Detection. , 2017, ACS nano.

[19]  Linfang Cai,et al.  Electrochemiluminescent detection of Hg(II) by exploiting the differences in the adsorption of free T-rich oligomers and Hg(II) loaded T-rich oligomers on silica nanoparticles doped with Ru(bpy)32+ , 2016, Microchimica Acta.

[20]  S. Yao,et al.  Ultrasensitive electrochemical sensing of Hg2+ based on thymine-Hg2+-thymine interaction and signal amplification of alkaline phosphatase catalyzed silver deposition. , 2018, Biosensors & bioelectronics.

[21]  Hongying Zhu,et al.  Cellphone-based detection platform for rbST biomarker analysis in milk extracts using a microsphere fluorescence immunoassay , 2014, Analytical and Bioanalytical Chemistry.

[22]  Sai Bi,et al.  A target-initiated DNA network caged on magnetic particles for amplified chemiluminescence resonance energy transfer imaging of microRNA and targeted drug delivery. , 2016, Chemical communications.

[23]  Joachim Mayer,et al.  Au@Hg Nanoalloy Formation Through Direct Amalgamation: Structural, Spectroscopic, and Computational Evidence for Slow Nanoscale Diffusion , 2011 .

[24]  Guangming Zeng,et al.  Practical and regenerable electrochemical aptasensor based on nanoporous gold and thymine-Hg2+-thymine base pairs for Hg2+ detection. , 2017, Biosensors & bioelectronics.

[25]  R. Handy,et al.  Dose-dependent inorganic mercury absorption by isolated perfused intestine of rainbow trout, Oncorhynchus mykiss, involves both amiloride-sensitive and energy-dependent pathways. , 2005, Aquatic toxicology.

[26]  Sai Bi,et al.  Chemiluminescence resonance energy transfer imaging on magnetic particles for single-nucleotide polymorphism detection based on ligation chain reaction. , 2015, Biosensors & bioelectronics.

[27]  Shulin Zhao,et al.  Sensitive aptamer-based fluorescence polarization assay for mercury(II) ions and cysteine using silver nanoparticles as a signal amplifier , 2014, Microchimica Acta.

[28]  Hui-wang Ai,et al.  Fluorescent Sensors for Biological Applications , 2014, Sensors.

[29]  Xiaohong Zhou,et al.  T-T mismatch-driven biosensor using triple functional DNA-protein conjugates for facile detection of Hg2+. , 2016, Biosensors & bioelectronics.

[30]  Yi Lu,et al.  Rational design of "turn-on" allosteric DNAzyme catalytic beacons for aqueous mercury ions with ultrahigh sensitivity and selectivity. , 2007, Angewandte Chemie.

[31]  Ling Zhang,et al.  Label-free supersandwich electrochemiluminescence assay for detection of sub-nanomolar Hg2+. , 2011, Chemical communications.

[32]  Aydogan Ozcan,et al.  Emerging Technologies for Next-Generation Point-of-Care Testing. , 2015, Trends in biotechnology.

[33]  Sai Bi,et al.  Programmable strand displacement-based magnetic separation for simultaneous amplified detection of multiplex microRNAs by chemiluminescence imaging array. , 2017, Biosensors & bioelectronics.

[34]  Peng Miao,et al.  A novel electrochemical method to detect mercury (II) ions , 2009 .

[35]  B. Sridhar,et al.  Rapid Determination of Mercury in Plant and Soil Samples Using Inductively Coupled Plasma Atomic Emission Spectroscopy, a Comparative Study , 2006 .

[36]  Marcel Hollenstein,et al.  A highly selective DNAzyme sensor for mercuric ions. , 2008, Angewandte Chemie.

[37]  P. Peralta-Zamora,et al.  Multivariate optimization of mercury determination by flow injection-cold vapor generation-inductively coupled plasma optical emission spectrometry. , 2012, The Analyst.

[38]  S. Manahan,et al.  Atomic absorption analysis of strong heavy metal chelating agents in water and waste water. , 1973, Analytical chemistry.

[39]  Bin Du,et al.  Facile fabrication of an electrochemical aptasensor based on magnetic electrode by using streptavidin modified magnetic beads for sensitive and specific detection of Hg(2.). , 2016, Biosensors & bioelectronics.

[40]  Layered MnO₂ nanosheet as a label-free nanoplatform for rapid detection of mercury(II). , 2014, The Analyst.

[41]  Sai Bi,et al.  Linear light-scattering of gold nanostars for versatile biosensing of nucleic acids and proteins using exonuclease III as biocatalyst to signal amplification. , 2015, Biosensors & bioelectronics.

[42]  Yalin Tang,et al.  Visual detection of mercury(II) based on recognition of the G-quadruplex conformational transition by a cyanine dye supramolecule. , 2015, The Analyst.

[43]  Jin Sun,et al.  Elimination efficiency of different reagents for the memory effect of mercury using ICP-MS , 2006 .

[44]  Ting Yang,et al.  Mercury Speciation with Fluorescent Gold Nanocluster as a Probe. , 2018, Analytical chemistry.

[45]  Changzhong Jiang,et al.  Ultrasensitive Au Nanooctahedron Micropinball Sensor for Mercury Ions. , 2018, ACS applied materials & interfaces.

[46]  Sai Bi,et al.  Target-triggered cascade recycling amplification for label-free detection of microRNA and molecular logic operations. , 2016, Chemical communications.

[47]  Xin Meng,et al.  Smartphone based hand-held quantitative phase microscope using the transport of intensity equation method. , 2016, Lab on a chip.

[48]  Huan‐Tsung Chang,et al.  Detection of mercury(II) ions using colorimetric gold nanoparticles on paper-based analytical devices. , 2014, Analytical chemistry.

[49]  Dakota O'Dell,et al.  Rapid diagnostic testing platform for iron and vitamin A deficiency , 2017, Proceedings of the National Academy of Sciences.

[50]  A. Aziz,et al.  Recent advances in DNA-based electrochemical biosensors for heavy metal ion detection: A review. , 2017, Biosensors & bioelectronics.

[51]  Injae Shin,et al.  In vivo monitoring of mercury ions using a rhodamine-based molecular probe. , 2006, Journal of the American Chemical Society.

[52]  Derek Tseng,et al.  Fluorescent imaging of single nanoparticles and viruses on a smart phone. , 2013, ACS nano.

[53]  M Virta,et al.  Detection of organomercurials with sensor bacteria. , 2001, Analytical chemistry.

[54]  Fang-fang Wang,et al.  Heating enhanced sensitive and selective electrochemical detection of Hg2+ based on T-Hg2+-T structure and exonuclease III-assisted target recycling amplification strategy at heated gold disk electrode. , 2018, Biosensors & bioelectronics.

[55]  Guonan Chen,et al.  Photoinduced Electron Transfer Mediated by π-Stacked Thymine−Hg2+−Thymine Base Pairs , 2011 .

[56]  Hongying Zhu,et al.  Cost-effective and rapid blood analysis on a cell-phone. , 2013, Lab on a chip.

[57]  Highly Sensitive Fluorometric Hg2+ Biosensor with a Mercury(II)-Speci?c Oligonucleotide (MSO) Probe and Water-Soluble Graphene Oxide (WSGO) , 2011 .