HLA class I and class II allele distribution in the Bubi population from the island of Bioko (Equatorial Guinea).

We determined the HLA frequency distribution in a sample of 100 Bubi individuals born on the island of Bioko (Equatorial Guinea). HLA-A, -B and -C typing was performed by serology and PCR-SSP. DRB1/3/4/5, DQB1 and DQA1 alleles were determined by PCR-SSOP. The HLA allele distribution of this population group resembles those found in other Bantu-speaking groups; however, the higher frequency of A30, A32, B44, DRB1*1301 in the Bubi with respect to other Bantu groups and the absence of DR4 deserve special mention. The cloning and sequencing of class I and II genes in this population allowed the description of five new allelic variants: B*4407, Cw*0706, Cw*1801, Cw*1802 and DQB1*0612 and five confirmatory sequences: B*3910, B*5703, B*8101, Cw*1203 and Cw*1701. The following new HLA-C,B haplotypes have been found in Bubi: Cw*08-B*57, Cw*18-B*57, Cw*0302-B*53, Cw*07-B*53 and Cw*1601-B*63. The most frequent seven-locus haplotype is: A*30-Cw*17-B*42-DRB1*1102-DRB3*0202-DQA1*05-DQB1 *0301. In terms of genetic distance, the Bubi are closer to other Bantu groups than to West African populations.

[1]  M. Bunce,et al.  The novel HLA-Cw*1802 allele is associated with B*5703 in the Bubi population from Equatorial Guinea. , 1997, Tissue antigens.

[2]  M. J. Herrero,et al.  The complete primary structure of Cw*1701 reveals a highly divergent HLA class I molecule. , 1997, Tissue antigens.

[3]  M. Barnardo,et al.  High resolution HLA-C typing by PCR-SSP: identification of allelic frequencies and linkage disequilibria in 604 unrelated random UK Caucasoids and a comparison with serology. , 1996, Tissue antigens.

[4]  M. Bunce,et al.  Molecular cloning of two new HLA-C alleles: Cw*1801 and Cw*0706. , 1996, Tissue antigens.

[5]  J. García-Pacheco,et al.  Complete coding region of the new HLA-DQB1*0612 allele, obtained by RT-PCR. , 1996, Tissue antigens.

[6]  B. Dupont,et al.  Comparison of HLA-A antigen typing by serology with two polymerase chain reaction based DNA typing methods: implications for proficiency testing. , 1996, Tissue antigens.

[7]  P. Krausa,et al.  Genetic diversity of HLA-A2: evolutionary and functional significance. , 1996, Immunology today.

[8]  C. Rogier,et al.  HLA-A, -B, -C, -DR, -DQ typing in a population group of Senegal: distribution of HLA antigens and HLA-DRB1*13 and DRB1*11 subtyping by PCR using sequence-specific primers (PCR-SSP). , 1996, Tissue antigens.

[9]  M. E. Moreno,et al.  Molecular characterization of the new alleles HLA-B*8101 and B*4407. , 1996, Tissue antigens.

[10]  Arne Svejgaard,et al.  Nomenclature for Factors of the HLA System, 1995 , 1995, Vox sanguinis.

[11]  J. García-Pacheco,et al.  Routine HLA DRB/DQB oligonucleotide typing by a non-radioactive dot-blot micromethod. , 1995, Journal of immunological methods.

[12]  S. Yashiki,et al.  Distribution of HLA and haplotypes of Colombian and Jamaican black populations. , 1995, Tissue antigens.

[13]  M. Bunce,et al.  Comprehensive, serologically equivalent DNA typing for HLA-B by PCR using sequence-specific primers (PCR-SSP). , 1995, Tissue antigens.

[14]  C. Kaplan,et al.  HLA-A, B, C, DR and DQ polymorphism in Zaireans. , 1994, Tissue antigens.

[15]  J. Klein,et al.  The molecular descent of the major histocompatibility complex. , 1993, Annual review of immunology.

[16]  T. Sasazuki,et al.  DNA typing of HLA class II genes in B-lymphoblastoid cell lines homozygous for HLA. , 1992, Tissue antigens.

[17]  D. Kwiatkowski,et al.  Extensive genetic diversity in the HLA class II region of Africans, with a focally predominant allele, DRB1*1304. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[18]  D. Kwiatkowski,et al.  Interethnic genetic differentiation in Africa: HLA class I antigens in The Gambia. , 1992, American journal of human genetics.

[19]  P. Stastny,et al.  HLA class II haplotypes in Amerindians and in black North and South Americans. , 1991, Tissue antigens.

[20]  E. Riley,et al.  HLA-DR and -DQ gene polymorphism in West Africans is twice as extensive as in north European Caucasians: evolutionary implications. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[21]  Andrew J. McMichael,et al.  Common West African HLA antigens are associated with protection from severe malaria , 1991, Nature.

[22]  M. Oudshoorn,et al.  HLA frequencies in black Zimbabweans. , 1990, Tissue antigens.

[23]  M. Oudshoorn,et al.  HLA-A, B, C, DR and DQ polymorphisms in three South African population groups: South African Negroes, Cape Coloureds and South African Caucasoids. , 1988, Tissue antigens.

[24]  Shirley A. Miller,et al.  A simple salting out procedure for extracting DNA from human nucleated cells. , 1988, Nucleic acids research.

[25]  W. Ollier,et al.  HLA polymorphisms in Nigerians. , 2008, Tissue antigens.

[26]  M. Nei,et al.  Estimation of average heterozygosity and genetic distance from a small number of individuals. , 1978, Genetics.