Ultraviolet radiation drives methane emissions from terrestrial plant pectins.

Recent studies demonstrating an in situ formation of methane (CH(4)) within foliage and separate observations that soil-derived CH(4) can be released from the stems of trees have continued the debate about the role of vegetation in CH(4) emissions to the atmosphere. Here, a study of the role of ultraviolet (UV) radiation in the formation of CH(4) and other trace gases from plant pectins in vitro and from leaves of tobacco (Nicotiana tabacum) in planta is reported. Plant pectins were investigated for CH(4 )production under UV irradiation before and after de-methylesterification and with and without the singlet oxygen scavenger 1,4-diazabicyclo[2.2.2]octane (DABCO). Leaves of tobacco were also investigated under UV irradiation and following leaf infiltration with the singlet oxygen generator rose bengal or the bacterial pathogen Pseudomonas syringae. Results demonstrated production of CH(4), ethane and ethylene from pectins and from tobacco leaves following all treatments, that methyl-ester groups of pectin are a source of CH(4), and that reactive oxygen species (ROS) arising from environmental stresses have a potential role in mechanisms of CH(4) formation. Rates of CH(4 )production were lower than those previously reported for intact plants in sunlight but the results clearly show that foliage can emit CH(4) under aerobic conditions.

[1]  A. Vaughan.  The methane mystery , 2021, Nature.

[2]  Peter Bergamaschi,et al.  Tropical methane emissions: A revised view from SCIAMACHY onboard ENVISAT , 2008 .

[3]  D. Beerling,et al.  Missing methane emissions from leaves of terrestrial plants , 2008 .

[4]  F. Keppler,et al.  Effect of UV radiation and temperature on the emission of methane from plant biomass and structural components , 2008 .

[5]  T. Dueck,et al.  Are plants precursors for methane? , 2008, The New phytologist.

[6]  W. C. Mcroberts,et al.  Methoxyl groups of plant pectin as a precursor of atmospheric methane: evidence from deuterium labelling studies. , 2008, The New phytologist.

[7]  Peter Bergamaschi,et al.  Three years of greenhouse gas column-averaged dry air mole fractions retrieved from satellite - Part 2: Methane , 2008 .

[8]  Q. Schiermeier.  Methane mystery continues , 2007, Nature.

[9]  S. Ishizuka,et al.  Methane emissions from stems of Fraxinus mandshurica var. japonica trees in a floodplain forest , 2007 .

[10]  Hans Tømmervik,et al.  Prediction of the distribution of Arctic‐nesting pink‐footed geese under a warmer climate scenario , 2007 .

[11]  A. Schapendonk,et al.  No evidence for substantial aerobic methane emission by terrestrial plants: a 13C-labelling approach. , 2007, The New phytologist.

[12]  E. Dlugokencky,et al.  Airborne measurements indicate large methane emissions from the eastern Amazon basin , 2007 .

[13]  Q. Schiermeier.  Pollution decreases rainfall , 2007, Nature.

[14]  Peter Bergamaschi,et al.  Satellite chartography of atmospheric methane from SCIAMACHY on board ENVISAT: 2. Evaluation based on inverse model simulations , 2007 .

[15]  E. Sanhueza,et al.  Methane emission from tropical savanna Trachypogon sp. grasses , 2006 .

[16]  J. B. Miller,et al.  Contribution of anthropogenic and natural sources to atmospheric methane variability , 2006, Nature.

[17]  A. Austin,et al.  Plant litter decomposition in a semi-arid ecosystem controlled by photodegradation , 2006, Nature.

[18]  Manxiao Zhang,et al.  The relationship between reactive oxygen species and nitric oxide in ultraviolet-B-induced ethylene production in leaves of maize seedlings , 2006 .

[19]  Ilse Aben,et al.  Atmospheric constraints on global emissions of methane from plants , 2006 .

[20]  David C. Lowe,et al.  Stable isotopes provide revised global limits of aerobic methane emissions from plants , 2006 .

[21]  P. Crutzen,et al.  Methane production from mixed tropical savanna and forest vegetation in Venezuela , 2006 .

[22]  Patrick M. Crill,et al.  A source of methane from upland forests in the Brazilian Amazon , 2006 .

[23]  F. Keppler,et al.  Methane emissions from terrestrial plants under aerobic conditions , 2006, Nature.

[24]  H. Hirt,et al.  Reactive oxygen species: metabolism, oxidative stress, and signal transduction. , 2004, Annual review of plant biology.

[25]  O. Kovalchuk,et al.  Genome stability of vtc1, tt4, and tt5 Arabidopsis thaliana mutants impaired in protection against oxidative stress. , 2004, The Plant journal : for cell and molecular biology.

[26]  S. Madronich,et al.  Sensitivity of Biologically Active UV Radiation to Stratospheric Ozone Changes: Effects of Action Spectrum Shape and Wavelength Range¶ , 2003, Photochemistry and Photobiology.

[27]  Y. Takeuchi,et al.  Ethylene evolution from tobacco leaves irradiated with UV-B , 2002, Journal of Plant Research.

[28]  C. Gueymard.  Parameterized transmittance model for direct beam and circumsolar spectral irradiance , 2001 .

[29]  J. Dumville,et al.  Fingerprinting of polysaccharides attacked by hydroxyl radicals in vitro and in the cell walls of ripening pear fruit. , 2001, The Biochemical journal.

[30]  S. Fry.  Oxidative scission of plant cell wall polysaccharides by ascorbate-induced hydroxyl radicals. , 1998, The Biochemical journal.

[31]  H. Rennenberg,et al.  Black alder (Alnus Glutinosa (L.) Gaertn.) trees mediate methane and nitrous oxide emission from the soil to the atmosphere , 1998, Plant and Soil.

[32]  P. Lumsden,et al.  Impacts of elevated UV-B on forest ecosystems , 1997 .

[33]  A. Bent,et al.  Identification of Pseudomonas syringae pathogens of Arabidopsis and a bacterial locus determining avirulence on both Arabidopsis and soybean. , 1991, The Plant cell.

[34]  V. Barnett,et al.  Applied Linear Statistical Models , 1975 .

[35]  R. Setlow.  The wavelengths in sunlight effective in producing skin cancer: a theoretical analysis. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[36]  Alex E. S. Green,et al.  THE MIDDLE ULTRAVIOLET REACHING THE GROUND * , 1974 .

[37]  King Eo,et al.  Two simple media for the demonstration of pyocyanin and fluorescin. , 1954 .

[38]  X. Han,et al.  Aerobic methane emission from plants in the Inner Mongolia steppe. , 2008, Environmental science & technology.

[39]  Peter Bergamaschi,et al.  SATELLITE CHARTOGRAPHY OF ATMOSPHERIC METHANE FROM SCIAMACHY ONBOARD ENVISAT , 2007 .

[40]  Ü. Niinemets,et al.  How Important is Aerobic Methane Release by Plants , 2007 .

[41]  K. Newsham,et al.  Elevated UV-B radiation modifies the extractability of carbohydrates from leaf litter of Quercus robur , 2007 .

[42]  A. McLeod.  Outdoor supplementation systems for studies of the effects of increased UV-B radiation , 2004, Plant Ecology.

[43]  Stephan D. Flint,et al.  A biological spectral weighting function for ozone depletion research with higher plants , 2003 .

[44]  G. Seymour,et al.  Pectins and their manipulation , 2002 .

[45]  J. C. Sutherland,et al.  Action spectrum for DMA damage in alfalfa lowers predicted impact of ozone depletion , 1992, Nature.

[46]  W. Lonneman,et al.  Contamination from fluorocarbon films , 1981 .

[47]  M. Caldwell,et al.  Chapter 4 – SOLAR UV IRRADIATION AND THE GROWTH AND DEVELOPMENT OF HIGHER PLANTS , 1971 .

[48]  E. King,et al.  Two simple media for the demonstration of pyocyanin and fluorescin. , 1954, The Journal of laboratory and clinical medicine.