Use of isothermal microcalorimetry to monitor microbial activities.

Isothermal calorimetry measures the heat flow of biological processes, which is proportional to the rate at which a given chemical or physical process takes place. Modern isothermal microcalorimeters make measurements of less than a microwatt of heat flow possible. As a result, as few as 10 000-100 000 active bacterial cells in culture are sufficient to produce a real-time signal dynamically related to the number of cells present and their activity. Specimens containing bacteria need little preparation, and isothermal microcalorimetry (IMC) is a nondestructive method. After IMC measurements, the undisturbed samples can be evaluated by any other means desired. In this review, we present a basic description of microcalorimetry and examples of microbiological applications of IMC for medical and environmental microbiology. In both fields, IMC has been used to quantify microbial activity over periods of hours or even days. Finally, the recent development of highly parallel instruments (up to 48 channels) and the constantly decreasing costs of equipment have made IMC increasingly attractive for microbiology. Miniaturization of isothermal calorimeters provides an even wider range of possibilities.

[1]  Jordi Mas,et al.  Calorimetry of microbial growth using a thermopile based microreactor , 2005 .

[2]  Peter Kuhn,et al.  Enthalpy array analysis of enzymatic and binding reactions. , 2008, Analytical biochemistry.

[3]  L. Wadsö,et al.  Isothermal calorimetry for biological applications in food science and technology , 2009 .

[4]  G. Sparling.  Estimation of microbial biomass and activity in soil using microcalorimetry , 1983 .

[5]  A. U. Daniels,et al.  Microcalorimetry: a novel method for detection of microbial contamination in platelet products , 2007, Transfusion.

[6]  M. Raviv,et al.  Evaluating microbial activity in composts using microcalorimetry , 2004 .

[7]  D. Wirz,et al.  Isothermal micro calorimetry – a new method for MIC determinations: results for 12 antibiotics and reference strains of E. coli and S. aureus , 2009, BMC Microbiology.

[8]  W. Babel,et al.  Thermokinetic description of anaerobic growth of Halomonas halodenitrificans using a static microcalorimetric ampoule technique. , 2003, Journal of biotechnology.

[9]  P. Johansson,et al.  An isothermal microcalorimetric titration/perfusion vessel equipped with electrodes and spectrophotometer , 1999 .

[10]  A. Steinhuber,et al.  Performance of Microcalorimetry for Early Detection of Methicillin Resistance in Clinical Isolates of Staphylococcus aureus , 2009, Journal of Clinical Microbiology.

[11]  L. Yi,et al.  Microcalorimetric study of Staphylococcus aureus growth affected by selenium compounds , 2002 .

[12]  Yong-Jun Kim,et al.  Neisseria Meningitidis Detection Based on a Microcalorimetric Biosensor With a Split-Flow Microchannel , 2008, Journal of Microelectromechanical Systems.

[13]  M. Raviv,et al.  Methodological aspects of microcalorimetry used to assess the dynamics of microbial activity during composting. , 2009, Bioresource technology.

[14]  A. W. van Herwaarden,et al.  Overview of calorimeter chips for various applications , 2005 .

[15]  J. Vanfleteren,et al.  Assaying metabolic activity in ageing Caenorhabditis elegans , 2002, Mechanisms of Ageing and Development.

[16]  D. Wirz,et al.  Rapid Differentiation of Methicillin-Susceptible Staphylococcus aureus from Methicillin-Resistant S. aureus and MIC Determinations by Isothermal Microcalorimetry , 2008, Journal of Clinical Microbiology.

[17]  Q. Huang,et al.  Isothermal Microcalorimetry： A Review of Applications in Soil and Environmental Sciences , 2007 .

[18]  L. Wadsö.  Isothermal Microcalorimetry. Current problems and prospects , 2001 .

[19]  R. Weiss.  The solubility of nitrogen, oxygen and argon in water and seawater , 1970 .

[20]  A. Manz,et al.  An integrated silicon thermophile as biosensor for the thermal monitoring of glucose, urea and penicillin. , 1993, Biosensors & bioelectronics.

[21]  M. Bruschi,et al.  Thermodynamics of cleaning-up of waste waters polluted by chromium , 2002 .

[22]  Robert N. Goldberg,et al.  Standards in isothermal microcalorimetry (IUPAC Technical Report) , 2001 .

[23]  A. Bhagwat,et al.  ANAEROBIC METABOLISM IN LAKE WASHINGTON SEDIMENTS1 , 1973 .

[24]  Ingemar Wadsö,et al.  Trends in isothermal microcalorimetry , 1997 .

[25]  D. Rank,et al.  Simultaneous measurement of metabolic heat rate, CO2 production, and O2 consumption by microcalorimetry. , 1991, Analytical biochemistry.

[26]  M. Cooper.  Label-free screening of bio-molecular interactions , 2003, Analytical and bioanalytical chemistry.

[27]  F. Beese,et al.  Comparison of microbial properties measured by O2 consumption and microcalorimetry as bioindicators in forest soils , 1999 .

[28]  W. Cun-xin,et al.  Microcalorimetric study of virus infection; The effects of hyperthermia and 1b recombinant homo interferon on the infection process of BHK-21 cells by foot and mouth disease virus , 2005 .

[29]  I. Wadsö,et al.  Microcalorimetric observations of microbial activity in normal and acidified soils , 1979 .

[30]  G. Svehla,et al.  Biochemical and clinical applications of thermometric and thermal analysis , 1982 .

[31]  G. Graf,et al.  Heat Production. ATP Concentration and Electron Transport Activity of Marine Sediments , 1981 .

[32]  C. Airoldi,et al.  Microcalorimetric study of some Amazonian soils , 1999 .

[33]  L. Sun,et al.  Microcalorimetric studies on the antimicrobial actions of different cephalosporins , 2008 .

[34]  Santiago Fernández,et al.  Microcalorimetric determination of the cell specific heat rate in soils: relationship with the soil microbial population and biophysic significance , 2003 .

[35]  L. Tranvik,et al.  Depth distribution of active bacteria and bacterial activity in lake sediment. , 2003, FEMS microbiology ecology.

[36]  C. Airoldi,et al.  Comparison between microorganism counting and a calorimetric method applied to tropical soils , 2002 .

[37]  V. Baier,et al.  A new micro-fluid chip calorimeter for biochemical applications , 2006 .

[38]  L. Wadsö,et al.  Measurements on two mould fungi with a calorespirometric method , 2004 .

[39]  Pietro Rocculi,et al.  The potential of isothermal calorimetry in monitoring and predicting quality changes during processing and storage of minimally processed fruits and vegetables , 2005 .

[40]  P. Bataillard,et al.  Calorimetric sensing in bioanalytical chemistry : principles, applications and trends , 1993 .

[41]  H. Harms,et al.  Chip calorimetry for the monitoring of whole cell biotransformation. , 2006, Journal of biotechnology.

[42]  I. Wadsö.  Isothermal microcalorimetry in applied biology , 2002 .

[43]  LavoisierMM.,et al.  Mémoire sur la chaleur , 1921 .

[44]  J. Monod.  The Growth of Bacterial Cultures , 1949 .

[45]  A. Tan,et al.  Microcalorimetric study of antiviral effect of drug. , 1999, Journal of biochemical and biophysical methods.

[46]  W. Sand,et al.  Evaluation of biocide efficacy by microcalorimetric determination of microbial activity in biofilms. , 1998 .

[47]  A. G. Bell,et al.  Enthalpy arrays. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[48]  F. Beese,et al.  Use of microcalorimetry to study microbial activity during the transition from oxic to anoxic conditions , 2002, Biology and Fertility of Soils.

[49]  S. Tadigadapa,et al.  Calorimetric biosensors with integrated microfluidic channels. , 2004, Biosensors & bioelectronics.

[50]  Jens Völker,et al.  Universal constant for heat production in protists , 2009, Proceedings of the National Academy of Sciences.

[51]  I. Wadsö.  Characterization of microbial activity in soil by use of isothermal microcalorimetry , 2009 .

[52]  A. U. Daniels,et al.  Use of isothermal heat-conduction microcalorimetry (IHCMC) for the evaluation of synthetic biomaterials. , 2003, Journal of biomedical materials research. Part B, Applied biomaterials.