Dual role of parathyroid hormone in endothelial progenitor cells and marrow stromal mesenchymal stem cells

Hematopoietic stem cells derive regulatory information also from parathyroid hormone (PTH). To explore the possibility that PTH may have a role in regulation of other stem cells residing in bone marrow, such as mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) we assessed the effect of this hormone on the in vitro behavior of MSCs and EPCs. We evidenced that MSCs were much more responsive to PTH than EPCs. PTH increased the proliferation rate of MSCs with a diminution of senescence and apoptosis. Taken together, our results may suggest a protective effect of PTH on MSCs that reduces stress phenomena and preserve genome integrity. At the opposite, PTH did not modify the fate of EPCs in culture. J. Cell. Physiol. 222: 474–480, 2010. © 2009 Wiley‐Liss, Inc.

[1]  T. Zeng,et al.  Safety, tolerability and pharmacokinetic study of recombinant human parathyroid hormone [rhPTH (1-84)] in Chinese healthy volunteers , 2009, Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban.

[2]  H. Burt,et al.  Effects of continuous and pulsatile PTH treatments on rat bone marrow stromal cells. , 2009, Biochemical and biophysical research communications.

[3]  D. Scadden,et al.  Role of the Osteoblast Lineage in the Bone Marrow Hematopoietic Niches , 2009, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[4]  J. Pearson.  Endothelial progenitor cells – hype or hope? , 2009, Journal of thrombosis and haemostasis : JTH.

[5]  C. Napoli,et al.  Targeting vascular niche by parathyroid hormone. , 2008, Current medicinal chemistry.

[6]  K. Hirschi,et al.  Assessing identity, phenotype, and fate of endothelial progenitor cells. , 2008, Arteriosclerosis, thrombosis, and vascular biology.

[7]  R. David,et al.  Parathyroid hormone effectively induces mobilization of progenitor cells without depletion of bone marrow. , 2008, Experimental hematology.

[8]  C. Napoli,et al.  Therapeutic targeting of the stem cell niche in experimental hindlimb ischemia , 2008, Nature Clinical Practice Cardiovascular Medicine.

[9]  S. Grundmann,et al.  Parathyroid hormone treatment after myocardial infarction promotes cardiac repair by enhanced neovascularization and cell survival. , 2008, Cardiovascular research.

[10]  W. Franz,et al.  Primary hyperparathyroidism is associated with increased circulating bone marrow-derived progenitor cells. , 2007, American journal of physiology. Endocrinology and metabolism.

[11]  C. Napoli,et al.  Therapeutic approaches in vascular repair induced by adult bone marrow cells and circulating progenitor endothelial cells. , 2007, Current pharmaceutical design.

[12]  D. Sinclair,et al.  The role of nuclear architecture in genomic instability and ageing , 2007, Nature Reviews Molecular Cell Biology.

[13]  J. Campisi,et al.  Cellular senescence: when bad things happen to good cells , 2007, Nature Reviews Molecular Cell Biology.

[14]  C. Napoli,et al.  Bone marrow cell-mediated cardiovascular repair: potential of combined therapies. , 2007, Trends in molecular medicine.

[15]  M. Konopleva,et al.  Phase I trial of parathyroid hormone to facilitate stem cell mobilization. , 2007, Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation.

[16]  Alexei A. Sharov,et al.  Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells , 2007, Nature Cell Biology.

[17]  A. Giordano,et al.  From the laboratory bench to the patient's bedside: An update on clinical trials with mesenchymal stem cells , 2007, Journal of cellular physiology.

[18]  Alexei A. Sharov,et al.  Dissecting Oct3/4-Regulated Gene Networks in Embryonic Stem Cells by Expression Profiling , 2006, PloS one.

[19]  Zining Wu,et al.  Intermittent treatment with parathyroid hormone (PTH) as well as a non-peptide small molecule agonist of the PTH1 receptor inhibits adipocyte differentiation in human bone marrow stromal cells. , 2006, Bone.

[20]  A. Giordano,et al.  The retinoblastoma gene is involved in multiple aspects of stem cell biology , 2006, Oncogene.

[21]  S. Yamanaka,et al.  Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors , 2006, Cell.

[22]  F. Sellke,et al.  Role of Stromal-Derived Factor-1&agr; in the Induction of Circulating CD34+CXCR4+ Progenitor Cells After Cardiac Surgery , 2006, Circulation.

[23]  F. Bosman,et al.  Immunohistochemical Expression of Endothelial Markers CD31, CD34, von Willebrand Factor, and Fli-1 in Normal Human Tissues , 2006, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[24]  K. Le Blanc,et al.  Mesenchymal stem cells: progress toward promise. , 2005, Cytotherapy.

[25]  Frederick W. Alt,et al.  DNA Repair, Genome Stability, and Aging , 2005, Cell.

[26]  N. Drize,et al.  Effect of parathyroid hormone PTH (1-34) on hemopoietic and stromal stem cells , 2004, Bulletin of Experimental Biology and Medicine.

[27]  M. Tendera,et al.  Mobilization of CD34/CXCR4+, CD34/CD117+, c-met+ Stem Cells, and Mononuclear Cells Expressing Early Cardiac, Muscle, and Endothelial Markers Into Peripheral Blood in Patients With Acute Myocardial Infarction , 2004, Circulation.

[28]  H. Hassan,et al.  The role of BMP-6, IL-6, and BMP-4 in mesenchymal stem cell-dependent bone development: effects on osteoblastic differentiation induced by parathyroid hormone and vitamin D(3). , 2004, Stem cells and development.

[29]  Haiyang Huang,et al.  Identification of the haematopoietic stem cell niche and control of the niche size , 2003, Nature.

[30]  D. Melton,et al.  "Stemness": Transcriptional Profiling of Embryonic and Adult Stem Cells , 2002, Science.

[31]  Thomas B. L. Kirkwood,et al.  Stress, DNA damage and ageing — an integrative approach , 2001, Experimental Gerontology.

[32]  U. Galderisi,et al.  Differentiation and apoptosis of neuroblastoma cells: Role of N‐myc gene product , 1999, Journal of cellular biochemistry.

[33]  A. Giaccia,et al.  The complexity of p53 modulation: emerging patterns from divergent signals. , 1998, Genes & development.

[34]  D. Prockop.  Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues , 1997, Science.

[35]  David W. Rowe,et al.  Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche , 2009, Nature.

[36]  J. Frisén,et al.  Deconstructing stemness , 2005, The EMBO journal.

[37]  M. Tendera,et al.  Mobilization of CD 34 / CXCR 4 , CD 34 / CD 117 , c-met Stem Cells , and Mononuclear Cells Expressing Early Cardiac , Muscle , and Endothelial Markers Into Peripheral Blood in Patients With Acute Myocardial Infarction , 2004 .

[38]  E. Deryugina,et al.  The stromal cells' guide to the stem cell universe , 1995, Stem cells.