论文信息 - Extraction of Acanthamoeba DNA by Use of Chelex Resin

Extraction of Acanthamoeba DNA by Use of Chelex Resin

Acanthamoeba keratitis (AK) is a rare corneal infection caused by the free-living amoeba Acanthamoeba ([1][1]). Diagnosis of AK is based on the culture of corneal scrapings or detection of the organism in pathology specimens ([1][1]). Culture positivity and histology may require a long time (3 to 7

A. Iovieno | S. Kilvington | Darlene Miller | E. Alfonso | J. Lonnen

[1] M. Fini,et al. Molecular Identification of T4 and T5 Genotypes in Isolates from Acanthamoeba Keratitis Patients , 2009, Journal of Clinical Microbiology.

[2] S. Kilvington,et al. Acanthamoeba keratitis: diagnosis and treatment update 2009. , 2009, American journal of ophthalmology.

[3] A. Cheng,et al. A simple and rapid method for extracting bacterial DNA from intestinal microflora for ERIC-PCR detection. , 2008, World journal of gastroenterology.

[4] L. Laroche,et al. Resistance of Acanthamoeba to classic DNA extraction methods used for the diagnosis of corneal infections , 2007, British Journal of Ophthalmology.

[5] H. Inoue,et al. Chelating Resin-Based Extraction of DNA from Dental Pulp and Sex Determination from Incinerated Teeth with Y-Chromosomal Alphoid Repeat and Short Tandem Repeats , 2002, The American journal of forensic medicine and pathology.

[6] N. Khan,et al. Molecular Tools for Speciation and Epidemiological Studies of Acanthamoeba , 2002, Current Microbiology.