Characterization and application of Raman labels for confocal Raman microspectroscopic detection of cellular proteins in single cells.

A method using confocal Raman microspectroscopy for the detection of cellular proteins in single intact cells was developed. Two approaches were used to improve the detection of these cellular components. First, compounds with high Raman scattering were investigated for potential use as Raman labels. Raman labels were conjugated to either biomolecules or biotin and used as markers in the detection of cellular enzymes and receptors. Second, silver colloids were used to increase the surface-enhanced Raman scatter (SERS) of these Raman labels. Cresyl violet and dimethylaminoazobenzene are Raman labels that provide very sensitive SERS detection by a confocal Raman microscope with a HeNe laser at wavelength of 632.8 nm. The detection of 12-lipoxygenase and cyclooxygenase-1 in single bovine coronary artery endothelial cells and the binding of angiotensin II to its receptors in zona glomerulosa cells was demonstrated.

[1]  J L Luke,et al.  Raman chemical imaging: histopathology of inclusions in human breast tissue. , 1996, Analytical chemistry.

[2]  T. Cotton,et al.  Immunoassay employing surface-enhanced Raman spectroscopy. , 1989, Analytical biochemistry.

[3]  J. W. Woods,et al.  Subcellular Localization of Prostaglandin Endoperoxide H Synthases-1 and -2 by Immunoelectron Microscopy* , 1998, The Journal of Biological Chemistry.

[4]  A. Mahadevan-Jansen,et al.  Near‐Infrared Raman Spectroscopy for In Vitro Detection of Cervical Precancers , 1998 .

[5]  M S Feld,et al.  Determination of human coronary artery composition by Raman spectroscopy. , 1997, Circulation.

[6]  R. Dasari,et al.  Biochemical composition of human peripheral arteries examined with near-infrared Raman spectroscopy. , 1998, Journal of vascular surgery.

[7]  N. Ramanujam,et al.  Development of a Fiber Optic Probe to Measure NIR Raman Spectra of Cervical Tissue In Vivo , 1998, Photochemistry and photobiology.

[8]  K. Watanabe,et al.  Immunohistochemical study of arachidonate 12-lipoxygenase in porcine tissues. , 1989, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[9]  M. Feld,et al.  Raman Spectroscopy and Fluorescence Photon Migration for Breast Cancer Diagnosis and Imaging , 1998, Photochemistry and photobiology.

[10]  W. Campbell,et al.  Nitric Oxide Inhibits Aldosterone Synthesis by a Guanylyl Cyclase-Independent Effect* * This work was supported by grants from the NHLBI (HL-52159 and HL-54717). , 1998, Endocrinology.

[11]  A F van der Steen,et al.  Intravascular ultrasound combined with Raman spectroscopy to localize and quantify cholesterol and calcium salts in atherosclerotic coronary arteries. , 2000, Arteriosclerosis, thrombosis, and vascular biology.

[12]  M Fitzmaurice,et al.  Histopathology of human coronary atherosclerosis by quantifying its chemical composition with Raman spectroscopy. , 1998, Circulation.

[13]  M. Manfait,et al.  Selective analysis of antitumor drug interaction with living cancer cells as probed by surface-enhanced Raman spectroscopy , 2004, European Biophysics Journal.

[14]  M. Manfait,et al.  Intracellular molecular interactions of antitumor drug amsacrine (m‐AMSA) as revealed by surface‐enhanced Raman spectroscopy , 1996, FEBS letters.

[15]  D H Kohn,et al.  Raman spectroscopic imaging markers for fatigue-related microdamage in bovine bone. , 2000, Analytical chemistry.

[16]  M. Long,et al.  Three-dimensional cellular development is essential for ex vivo formation of human bone , 2000, Nature Biotechnology.

[17]  T. Vo‐Dinh,et al.  Measurement of DNA adducts using surface-enhanced Raman spectroscopy. , 1993, Journal of toxicology and environmental health.

[18]  R. Panek,et al.  Subclasses of angiotensin II binding sites and their functional significance. , 1990, Molecular pharmacology.

[19]  D. H. Kohn,et al.  Ultrastructural Changes Accompanying the Mechanical Deformation of Bone Tissue: A Raman Imaging Study , 2003, Calcified Tissue International.

[20]  M Fitzmaurice,et al.  Diagnosis of human coronary atherosclerosis by morphology-based Raman spectroscopy. , 2001, Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology.

[21]  Y. Kraan,et al.  Intracellular carotenoid levels measured by Raman microspectroscopy: Comparison of lymphocytes from lung cancer patients and healthy individuals , 1997, International journal of cancer.

[22]  Jian Ling,et al.  Direct Raman imaging techniques for study of the subcellular distribution of a drug. , 2002, Applied optics.

[23]  G. Puppels,et al.  Raman spectroscopy for quantifying cholesterol in intact coronary artery wall. , 1998, Atherosclerosis.

[24]  J. Otto,et al.  Different Intracellular Locations for Prostaglandin Endoperoxide H Synthase-1 and −2 (*) , 1995, The Journal of Biological Chemistry.

[25]  T. Gansler,et al.  Characterization of human breast biopsy specimens with near-IR Raman spectroscopy. , 1994, Analytical chemistry.

[26]  P. White,et al.  Characterization of the Surface of a Citrate-Reduced Colloid Optimized for Use as a Substrate for Surface-Enhanced Resonance Raman Scattering , 1995 .

[27]  R. Sanderson,et al.  Modification of enzyme-conjugated streptavidin-biotin western blot technique to avoid detection of endogenous biotin-containing proteins. , 1999, BioTechniques.

[28]  M Fitzmaurice,et al.  Raman microspectroscopy of human coronary atherosclerosis: biochemical assessment of cellular and extracellular morphologic structures in situ. , 2001, Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology.

[29]  V. Fuster,et al.  Clinical Imaging of the High-Risk or Vulnerable Atherosclerotic Plaque , 2001, Circulation research.

[30]  Michael D Morris,et al.  Mineralization of Developing Mouse Calvaria as Revealed by Raman Microspectroscopy , 2002, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[31]  T. Vo‐Dinh,et al.  Surface-enhanced Raman gene probe for HIV detection. , 1998, Analytical chemistry.

[32]  J. Starkey,et al.  Heme compounds in dinosaur trabecular bone. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[33]  W. Campbell,et al.  Characterization of normal and malignant human hepatocytes by Raman microspectroscopy. , 1996, Cancer letters.

[34]  Landulfo Silveira,et al.  Correlation between near‐infrared Raman spectroscopy and the histopathological analysis of atherosclerosis in human coronary arteries , 2002, Lasers in surgery and medicine.

[35]  T. B. Bakker Schut,et al.  Discriminating basal cell carcinoma from its surrounding tissue by Raman spectroscopy. , 2002, The Journal of investigative dermatology.

[36]  V. Kalasinsky,et al.  Pathological and biophysical findings associated with silicone breast implants: a study of capsular tissues from 86 cases. , 1997, Plastic and reconstructive surgery.

[37]  J. J. Freeman,et al.  Raman Spectroscopic Detection of Changes in Bioapatite in Mouse Femora as a Function of Age and In Vitro Fluoride Treatment , 2001, Calcified Tissue International.

[38]  W. Campbell,et al.  Raman microspectroscopy of intracellular cholesterol crystals in cultured bovine coronary artery endothelial cells. , 1997, Journal of lipid research.

[39]  N. Ueda,et al.  Arachidonate 12-lipoxygenases. , 1997, Progress in lipid research.

[40]  L. A. Shitova,et al.  Confocal raman microspectroscopy and imaging study of theraphthal in living cancer cells. , 2000, Biophysical journal.

[41]  K. Morita,et al.  Role of transcription factor Egr-1 in phorbol ester-induced phenylethanolamine N-methyltransferase gene expression , 1995, The Journal of Biological Chemistry.

[42]  T. Vo‐Dinh,et al.  Surface-enhanced Raman gene probes. , 1994, Analytical chemistry.

[43]  F. Mullick,et al.  Laser-Raman microprobe identification of inclusions in capsules associated with silicone gel breast implants. , 1999, Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc.

[44]  G. Daculsi,et al.  Application of FT-IR microspectroscopy to the study of an injectable composite for bone and dental surgery. , 1998, Journal of biomedical materials research.