DETERMINATION OF GIBBERELLIC ACID FROM Rhizobium (MAIZE) BY THIN LAYER CHROMATOGRAPHY

The present study was conducted in order to know the production and optimization of the Gibberellic acid produced by Rhizobium isolated from the maize plants rhizopheric soil Yeast extract mannitol agar medium was used for the isolation of Rhizobium and identified by various physicochemical parameters. The production of Gibberellic acid was identified by Thin layer chromatography.The efficient strain was used for the production and extraction of Gibberellic acid by using ethyl acetate and Tween 80 using spectrophotometer at various incubation time. Optimization of media (carbon and nitrogen sources) were made for improved yield of gibberellic acid production.

[1]  M. Marziah,et al.  The effect of rhizobacterial inoculation on growth and nutrient accumulation of tissue-cultured banana plantlets under low N-fertilizer regime , 2009 .

[2]  Y. Sertdemir,et al.  The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX). , 2009 .

[3]  E. Ong,et al.  Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry after solid-phase extraction. , 2008, Analytica chimica acta.

[4]  B. Touraine,et al.  Plant growth-promoting bacteria and nitrate availability: impacts on root development and nitrate uptake. , 2003, Journal of experimental botany.

[5]  J. Ladha,et al.  Rhizobial communication with rice roots: Induction of phenotypic changes, mode of invasion and extent of colonization , 1997, Plant and Soil.

[6]  R. Lalande,et al.  Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: Effect on radishes (Raphanus sativus L.) , 2004, Plant and Soil.

[7]  A. A. Zavalin,et al.  Use of Nitrogen Fixing Bacteria Inoculants as a Substitute for Nitrogen Fertiliser for Dryland Graminaceous Crops: Progress Made, Mechanisms of Action and Future Potential , 2003 .

[8]  E. Martínez-Romero,et al.  Advances in Rhizobium Research , 2002 .

[9]  P. S. Basu,et al.  Production of Indole Acetic Acid in Culture by a Rhizobium Species from the Root Nodules of a Monocotyledonous Tree, Roystonea regia , 2001 .

[10]  A. Durand,et al.  Production of gibberellic acid by fed-batch solid state fermentation in an aseptic pilot-scale reactor , 1997 .

[11]  S. Lapage,et al.  Biochemical Tests for Identification of Medical Bacteria , 1976 .

[12]  J. Vincent.  A manual for the practical study of root-nodule bacteria , 1971 .

[13]  G. G. Pohlman.  CHANGES PRODUCED IN NITROGENOUS COMPOUNDS BY RHIZOBIUM MELILOTI AND RHIZOBIUM JAPONICUM , 1931 .