Functional Recovery of Axonal Injury Induced by Gelatin-Hydrogel Film and PRP: An Initial Study in Rats

In today’s society, axonal regeneration is considered very important because of high incidence of nerve injuries. The injury may cause vast damage of extracellular matrix leading to the condition wherein cell lost their environment to live. In this situation, new strategies by the introduction of scaffold to function as synthetic extracellular matrix are needed. In the field of tissue engineering, a large variety of carriers and matrices have been utilized as a scaffold to direct axonal growth. In this study, a new strategy by the combined application of gelatin hydrogel film and Platelet Rich Plasma (PRP) to induce functional recovery of sciatic nerve crushed injury was investigated in Wistar rat model. Assessment of motorist and sensory functions were done to evaluate the recovery processes. It was found that the application of gelatin hydrogel film with controlled release manner combined with PRP results faster axonal regeneration. Further research by different injury model and other investigation factors are needed to confirm the effectiveness of the strategy.

[1]  M. Ravalji.  Pathobiology of neuropathic pain , 2015 .

[2]  T. Deliberador,et al.  A standardized research protocol for platelet-rich plasma (PRP) preparation in rats , 2011 .

[3]  Y. Tabata,et al.  Topical insulin-like growth factor 1 treatment using gelatin hydrogels for glucocorticoid-resistant sudden sensorineural hearing loss: a prospective clinical trial , 2010, BMC medicine.

[4]  D. Muir,et al.  The potentiation of peripheral nerve sheaths in regeneration and repair , 2010, Experimental Neurology.

[5]  Y. Tabata,et al.  Basic fibroblast growth factor combined with biodegradable hydrogel promotes healing of facial nerve after compression injury: An experimental study , 2010, Acta oto-laryngologica.

[6]  J. van den Dolder,et al.  Bone regenerative properties of rat, goat and human platelet-rich plasma. , 2009, International journal of oral and maxillofacial surgery.

[7]  L. Sarikcioglu,et al.  Walking track analysis: an assessment method for functional recovery after sciatic nerve injury in the rat. , 2009, Folia morphologica.

[8]  A. Marti-Carvajal,et al.  Efficacy and safety of the use of autologous plasma rich in platelets for tissue regeneration: a systematic review , 2009, Transfusion.

[9]  S. Hayakawa.  Nerve Regeneration Guided by Chitosan Hybrid Membranes , 2009 .

[10]  Yasuhiko Tabata,et al.  Translational research in tissue engineering: experiences and lessons learned from Japan , 2008 .

[11]  Coert Jh,et al.  Synthetic nerve guide implants in humans: a comprehensive survey. , 2007 .

[12]  G. Gagnon,et al.  Activation of human platelet-rich plasmas: effect on growth factors release, cell division and in vivo bone formation. , 2007, Clinical oral implants research.

[13]  Y. Tabata,et al.  A novel approach to therapeutic angiogenesis for patients with critical limb ischemia by sustained release of basic fibroblast growth factor using biodegradable gelatin hydrogel: an initial report of the phase I-IIa study. , 2007, Circulation journal : official journal of the Japanese Circulation Society.

[14]  Y. Okita,et al.  Controlled Release of Basic Fibroblast Growth Factor From Gelatin Hydrogel Sheet Improves Structural and Physiological Properties of Vein Graft in Rat , 2007 .

[15]  原口 知則.  Controlled release of basic fibroblast growth factor from gelatin hydrogel sheet improves structural and physiological properties of vein graft in rat , 2007 .

[16]  B. Schlosshauer,et al.  Synthetic Nerve Guide Implants in Humans: A Comprehensive Survey , 2006, Neurosurgery.

[17]  Y. Tabata,et al.  Tissue engineering by modulated gene delivery. , 2006, Advanced drug delivery reviews.

[18]  Yoshito Ikada,et al.  Tissue Engineering: Fundamentals and Applications , 2006 .

[19]  筏 義人.  Tissue engineering : fundamentals and applications , 2006 .

[20]  K. Nandakumar,et al.  Applications of Platelet Rich Plasma for Regenerative Therapy in Periodontics , 2006 .

[21]  M. Shoichet,et al.  Peripheral nerve regeneration through guidance tubes , 2004, Neurological research.

[22]  Christine E Schmidt,et al.  Neural tissue engineering: strategies for repair and regeneration. , 2003, Annual review of biomedical engineering.

[23]  M. Zimmermann,et al.  Pathobiology of neuropathic pain. , 2001, European journal of pharmacology.

[24]  M. Meek,et al.  Functional evaluation of peripheral nerve regeneration in the rat: walking track analysis , 2001, Journal of Neuroscience Methods.

[25]  N. Mazzer,et al.  Correlation between functional index and morphometry to evaluate recovery of the rat sciatic nerve following crush injury: experimental study. , 2001, Journal of reconstructive microsurgery.

[26]  R. Marx,et al.  Platelet-rich plasma (PRP): what is PRP and what is not PRP? , 2001, Implant dentistry.

[27]  P. D. Wall,et al.  Autotomy following peripheral nerve lesions: experimental anesthesia dolorosa , 1979, Pain.