A second thrombospondin gene in the mouse is similar in organization to thrombospondin 1 but does not respond to serum.

A second, expressed thrombospondin (TSP) gene, Thbs2, has been identified in the mouse. The exon/intron organization of Thbs2 is highly conserved in comparison with Thbs1 in that exon size and the pattern of interruption of the reading frame by introns are preserved, but there is a marked divergence in coding sequence, primarily in the first 7 exons. On the other hand, the DNA and translated amino acid sequences of exons coding for the type I, II, and III repeats in the two TSPs are far better conserved. Thbs2 is located on chromosome 17, band A3, whereas Thbs1 was found on chromosome 2, band F. In marked contrast to Thbs1, the Thbs2 gene is not induced by serum in NIH 3T3 cells; promoter sequences in the two genes are also very different. It is therefore likely that the two TSPs perform related but distinct functions.

[1]  P. Bornstein,et al.  Extracellular proteins that modulate cell-matrix interactions. SPARC, tenascin, and thrombospondin. , 1991, The Journal of biological chemistry.

[2]  F. W. Wolf,et al.  A second, expressed thrombospondin gene (Thbs2) exists in the mouse genome. , 1991, The Journal of biological chemistry.

[3]  J. Lawler,et al.  Cloning and sequencing of chicken thrombospondin. , 1991, The Journal of biological chemistry.

[4]  D. Jaye,et al.  The properdin-like type I repeats of human thrombospondin contain a cell attachment site , 1991, The Journal of cell biology.

[5]  R. Nachman,et al.  Cellular attachment to thrombospondin. Cooperative interactions between receptor systems. , 1991, The Journal of biological chemistry.

[6]  P. Bornstein,et al.  Thrombospondin gene expression is associated with mitogenesis in 3T3 cells: Induction by basic fibroblast growth factor , 1990, Journal of cellular physiology.

[7]  P. Bornstein,et al.  Characterization of the mouse thrombospondin gene and evaluation of the role of the first intron in human gene expression. , 1990, The Journal of biological chemistry.

[8]  C. Disteche,et al.  Mapping of the thrombospondin gene to human chromosome 15 and mouse chromosome 2 by in situ hybridization. , 1990, Genomics.

[9]  R. Eddy,et al.  Structure and chromosomal localization of the human thrombospondin gene. , 1990, Genomics.

[10]  M. Höök,et al.  Thrombospondin modulates focal adhesions in endothelial cells , 1989, The Journal of cell biology.

[11]  Carol D. Laherty,et al.  Characterization of the promoter region of the human thrombospondin gene. DNA sequences within the first intron increase transcription. , 1989, The Journal of biological chemistry.

[12]  P. Kaesberg,et al.  Chinese hamster ovary cell adhesion to human platelet thrombospondin is dependent on cell surface heparan sulfate proteoglycan. , 1989, The Journal of clinical investigation.

[13]  D. Mosher,et al.  Heparan sulfate-mediated binding of epithelial cell surface proteoglycan to thrombospondin. , 1989, The Journal of biological chemistry.

[14]  T. Deckwerth,et al.  Complete thrombospondin mRNA sequence includes potential regulatory sites in the 3' untranslated region , 1989, The Journal of cell biology.

[15]  D. Adler,et al.  Cloning of a complementary DNA encoding a new mouse B lymphocyte differentiation antigen, homologous to the human B1 (CD20) antigen, and localization of the gene to chromosome 19. , 1988, Journal of immunology.

[16]  P. Bornstein,et al.  Structural analysis and expression of the human thrombospondin gene promoter. , 1988, The Journal of biological chemistry.

[17]  V. Dixit,et al.  Unique distribution of the extracellular matrix component thrombospondin in the developing mouse embryo , 1988, The Journal of cell biology.

[18]  R. Hynes,et al.  Cell attachment to thrombospondin: the role of ARG-GLY-ASP, calcium, and integrin receptors , 1988, The Journal of cell biology.

[19]  P. Bornstein,et al.  The first intron of the alpha 1(I) collagen gene contains several transcriptional regulatory elements. , 1988, The Journal of biological chemistry.

[20]  V. Dixit,et al.  Cell surface thrombospondin is functionally essential for vascular smooth muscle cell proliferation , 1988, The Journal of cell biology.

[21]  P. Goodfellow,et al.  The human homologue of the mouse t‐complex gene, TCP1, is located on chromosome 6 but is not near the HLA region. , 1987, The EMBO journal.

[22]  V. Dixit,et al.  Induction of thrombospondin messenger RNA levels occurs as an immediate primary response to platelet-derived growth factor. , 1987, The Journal of biological chemistry.

[23]  L. Patthy,et al.  Intron‐dependent evolution: Preferred types of exons and introns , 1987, FEBS letters.

[24]  J. Barnwell,et al.  Isolation of the thrombospondin membrane receptor. , 1987, The Journal of clinical investigation.

[25]  J. Ragoussis,et al.  Assignment of the TCP1 locus to the long arm of human chromosome 6 by in situ hybridization. , 1987, Cytogenetics and cell genetics.

[26]  P. Sharp,et al.  Splicing of messenger RNA precursors. , 1987, Cold Spring Harbor symposia on quantitative biology.

[27]  P. Bornstein,et al.  Partial amino acid sequence of human thrombospondin as determined by analysis of cDNA clones: homology to malarial circumsporozoite proteins. , 1986, Biochemistry.

[28]  R. Hynes,et al.  The structure of human thrombospondin, an adhesive glycoprotein with multiple calcium-binding sites and homologies with several different proteins , 1986, The Journal of cell biology.

[29]  R. Nachman,et al.  Human brain glial cells synthesize thrombospondin. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[30]  P. Bornstein,et al.  Platelet-derived growth factor and heparin-like glycosaminoglycans regulate thrombospondin synthesis and deposition in the matrix by smooth muscle cells , 1985, The Journal of cell biology.

[31]  V. Dixit,et al.  The platelet glycoprotein thrombospondin binds specifically to sulfated glycolipids. , 1985, The Journal of biological chemistry.

[32]  P. Bornstein,et al.  Light microscopic immunolocation of thrombospondin in human tissues. , 1985, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[33]  D. Melton,et al.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. , 1984, Nucleic acids research.

[34]  V. Dixit,et al.  Isolation and characterization of a heparin-binding domain from the amino terminus of platelet thrombospondin. , 1984, The Journal of biological chemistry.

[35]  W. Gehring,et al.  Genomic and cDNA clones of the homeotic locus Antennapedia in Drosophila. , 1983, The EMBO journal.

[36]  L. Silver,et al.  A major testicular cell protein specified by a mouse T/t complex gene , 1979, Cell.

[37]  P. Majerus,et al.  Isolation and properties of a thrombin-sensitive protein of human platelets. , 1972, The Journal of biological chemistry.