In vitro Cytotoxicity and Antimicrobial Activity of Biosurfactant Produced by Pseudomonas aeruginosa Strain PB3A

In the present study Pseudomonas aeruginosa PB3A strain isolated from oil contaminated soil was exploited for the biosurfactant production. Effect of growth parameters for the biosurfactant production were studied which revealed their optimum production occurs at pH 7, with a temperature of 37°C and optimum incubation time of 48 h. Further the characterization studied of partially purified biosurfactants by TLC and FTIR confirms the presence of biosurfactant production. Screening of antibacterial and anticancer studies also reveals that, the extracted biosurfactant effectively controlled the test pathogens and also suppress the proliferation of HeLa cells showing an IC50 value of 125 μg mLG1. The present study concludes the biosurfactant produced by Pseudomonas aeruginosa PB3A shows significant cytotoxicity activity which is of eco-friendly in nature.

[1]  Markus Michael Müller,et al.  Evaluation of rhamnolipid production capacity of Pseudomonas aeruginosa PAO1 in comparison to the rhamnolipid over-producer strains DSM 7108 and DSM 2874 , 2011, Applied Microbiology and Biotechnology.

[2]  Z. Khalid,et al.  Improved production of biosurfactant by a Pseudomonas aeruginosa mutant using vegetable oil refinery wastes , 2006, Biodegradation.

[3]  J. Trevors,et al.  A drop-collapsing test for screening surfactant-producing microorganisms , 1991 .

[4]  Hui Peng,et al.  Characteristics of biosurfactant produced by Pseudomonas aeruginosa S6 isolated from oil-containing wastewater , 2009 .

[5]  K. Tsuge,et al.  Isolation of a gene essential for biosynthesis of the lipopeptide antibiotics plipastatin B1 and surfactin in Bacillus subtilis YB8 , 1996, Archives of Microbiology.

[6]  D. Kitamoto,et al.  Production of Glycolipid Biosurfactants, Cellobiose Lipids, by Cryptococcus humicola JCM 1461 and Their Interfacial Properties , 2011, Bioscience, biotechnology, and biochemistry.

[7]  Manoj Kumar,et al.  Enhancement of oil degradation by co-culture of hydrocarbon degrading and biosurfactant producing bacteria. , 2006, Polish journal of microbiology.

[8]  G. S. Kiran,et al.  Production of a new glycolipid biosurfactant from marine Nocardiopsis lucentensis MSA04 in solid-state cultivation. , 2010, Colloids and surfaces. B, Biointerfaces.

[9]  B. Chopade,et al.  Assessment of different screening methods for selecting biosurfactant producing marine bacteria , 2008 .

[10]  M. M. Assadi,et al.  Improved Production of Rhamnolipids by a Pseudomonas aeruginosa Mutant , 2004 .

[11]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[12]  Jo‐Shu Chang,et al.  Rhamnolipid production with indigenous Pseudomonas aeruginosa EM1 isolated from oil-contaminated site. , 2008, Bioresource technology.

[13]  I. Banat,et al.  Bioremediation of gasoline contaminated soil by a bacterial consortium amended with poultry litter, coir pith and rhamnolipid biosurfactant. , 2002, Bioresource technology.

[14]  V. Viji,et al.  Halomonas sp. BS4, A biosurfactant producing halophilic bacterium isolated from solar salt works in India and their biomedical importance , 2013, SpringerPlus.

[15]  C. Rosa,et al.  Characterization of new biosurfactant produced by Trichosporon montevideense CLOA 72 isolated from dairy industry effluents , 2009, Journal of basic microbiology.

[16]  Z. Matovic,et al.  Antiproliferative and Proapoptotic Activities of Methanolic Extracts from Ligustrum vulgare L. as an Individual Treatment and in Combination with Palladium Complex , 2012, International journal of molecular sciences.

[17]  A. Bodour,et al.  Structure and Characterization of Flavolipids, a Novel Class of Biosurfactants Produced by Flavobacterium sp. Strain MTN11 , 2004, Applied and Environmental Microbiology.

[18]  K. Ono,et al.  Screening of Microorganisms for Microbial Enhanced Oil Recovery Processes , 2000 .

[19]  Cumaraswamy Vipulanandan,et al.  Removal of Lead from Contaminated Water and Clay Soil Using a Biosurfactant , 2006 .

[20]  Sanket J. Joshi,et al.  Production of biosurfactant and antifungal compound by fermented food isolate Bacillus subtilis 20B. , 2008, Bioresource technology.

[21]  N. Youssef,et al.  Comparison of methods to detect biosurfactant production by diverse microorganisms. , 2004, Journal of microbiological methods.

[22]  B. Aslim,et al.  Biosurfactant production in sugar beet molasses by some Pseudomonas spp. , 2009, Journal of environmental biology.

[23]  A. Bodour,et al.  Application of a modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms , 1998 .

[24]  A. Giulietti,et al.  Isolation and selection of biosurfactant-producing bacteria , 1996, World journal of microbiology & biotechnology.

[25]  C. Mulligan,et al.  Selection of microbes producing biosurfactants in media without hydrocarbons , 1984 .

[26]  L. Ju,et al.  Rhamnolipid production by Pseudomonas aeruginosa under denitrification: effects of limiting nutrients and carbon substrates. , 2001, Biotechnology and bioengineering.

[27]  G. Jung,et al.  Streptofactin, a novel biosurfactant with aerial mycelium inducing activity from Streptomyces tendae Tü 901/8c , 1998 .

[28]  Fritz Wagner,et al.  New method for detecting rhamnolipids excreted by Pseudomonas species during growth on mineral agar , 1991 .

[29]  J. Bonmatin,et al.  Recent trends in the biochemistry of surfactin , 1999, Applied Microbiology and Biotechnology.

[30]  Kamaljeet Kaur Sekhon,et al.  Biosurfactant Production and Potential Correlation with Esterase Activity , 2012 .