Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide the fluorescent images of living cell membranes. The proposed microscope with the helps of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having shorter fluorophore lifetime. In comparison with one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. Combining with the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated the brighter and more contrast fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.

[1]  D. Toomre,et al.  Lighting up the cell surface with evanescent wave microscopy. , 2001, Trends in cell biology.

[2]  Björn Persson,et al.  Surface plasmon fluorescence immunoassay of free prostate-specific antigen in human plasma at the femtomolar level. , 2004, Analytical chemistry.

[3]  Chi-Hung Lin,et al.  Enhanced live cell membrane imaging using surface plasmon-enhanced total internal reflection fluorescence microscopy. , 2006, Optics express.

[4]  S. J. Chen,et al.  Enhancement of the resolution of surface plasmon resonance biosensors by control of the size and distribution of nanoparticles. , 2004, Optics letters.

[5]  Prashant K. Jain,et al.  Plasmonic photothermal therapy (PPTT) using gold nanoparticles , 2008, Lasers in Medical Science.

[6]  D. Axelrod.  Total internal reflection fluorescence microscopy in cell biology. , 2003, Methods in enzymology.

[7]  Jörg Enderlein,et al.  The efficiency of surface-plasmon coupled emission for sensitive fluorescence detection. , 2005, Optics express.

[8]  W. Betz,et al.  Imaging exocytosis and endocytosis , 1996, Current Opinion in Neurobiology.

[9]  T. Klar,et al.  Biomolecular Recognition Based on Single Gold Nanoparticle Light Scattering , 2003 .

[10]  A. Rohrbach,et al.  Observing secretory granules with a multiangle evanescent wave microscope. , 2000, Biophysical journal.

[11]  Andrew G. Glen,et al.  APPL , 2001 .

[12]  A A Friesem,et al.  Enhanced two-photon fluorescence excitation by resonant waveguide structures , 2004, Conference on Lasers and Electro-Optics, 2004. (CLEO)..

[13]  L. Hsu,et al.  WW domain-containing oxidoreductase: a candidate tumor suppressor. , 2007, Trends in molecular medicine.

[14]  Hans-Adolf-Krebs Weg.  Evanescent-field-induced two-photon fluorescence: excitation of macroscopic areas of planar waveguides , 2001 .

[15]  J. Heath,et al.  Hyaluronidase Induction of a WW Domain-containing Oxidoreductase That Enhances Tumor Necrosis Factor Cytotoxicity* , 2001, The Journal of Biological Chemistry.

[16]  Min Gu,et al.  Two-photon fluorescence scanning near-field microscopy based on a focused evanescent field under total internal reflection. , 2003, Optics letters.

[17]  Colette McDonagh,et al.  Plasmonic enhancement of fluorescence for sensor applications , 2005 .

[18]  Q. Hong,et al.  Zfra affects TNF-mediated cell death by interacting with death domain protein TRADD and negatively regulates the activation of NF-κB, JNK1, p53 and WOX1 during stress response , 2007, BMC Molecular Biology.

[19]  L. Novotný,et al.  Enhancement and quenching of single-molecule fluorescence. , 2006, Physical review letters.

[20]  N. Thompson,et al.  Theory for two-photon excitation in pattern photobleaching with evanescent illumination. , 1993, Biophysical chemistry.

[21]  D. Axelrod,et al.  Actin dynamics at the living cell submembrane imaged by total internal reflection fluorescence photobleaching. , 2000, Biophysical journal.

[22]  G. Truskey,et al.  Total internal reflection fluorescence microscopy (TIRFM). II. Topographical mapping of relative cell/substratum separation distances. , 1992, Journal of cell science.

[23]  Enrico Gratton,et al.  Mitigating thermal mechanical damage potential during two-photon dermal imaging. , 2004, Journal of biomedical optics.

[24]  J. Lakowicz,et al.  Directional two-photon induced surface plasmon-coupled emission. , 2005, Thin solid films.

[25]  R. M. Fulbright,et al.  Dynamics of nonspecific adsorption of insulin to erythrocyte membranes , 1993, Journal of Fluorescence.

[26]  E. Fort,et al.  Surface enhanced fluorescence , 2008 .

[27]  Nicolaas Bloembergen,et al.  Light waves at the boundary of nonlinear media , 1962 .

[28]  W H Weber,et al.  Energy transfer from an excited dye molecule to the surface plasmons of an adjacent metal. , 1979, Optics letters.

[29]  Seth R. Marder,et al.  Five Orders-of-Magnitude Enhancement of Two-Photon Absorption for Dyes on Silver Nanoparticle Fractal Clusters , 2002 .

[30]  H. Kano,et al.  Two-photon-excited fluorescence enhanced by a surface plasmon. , 1996, Optics letters.

[31]  J. Lakowicz,et al.  Metal-Enhanced Fluorescence Sensing , 2005 .

[32]  Wolfgang Knoll,et al.  Surface-Plasmon Field-Enhanced Fluorescence Spectroscopy , 2000 .