A reinterpretation of the nature of "lipovirus" cytopathogenicity.

cells were observed on inverted Petri plates overlaid with agar. Individual cells were photographed at 2-min intervals. The changes occurring in marked areas at the edge of a developing plaque were recorded at 4- and 10-hr intervals. Electron microscopy: The cells were prepared for electron microscopy by: (1) fixation in 1%-/0 osmium and embedment in methacrylate; (2) fixation in gluteraldehyde, post-fixation in osmium, staining in uranyl acetate, embedment in Epon, and post-staining with lead citrate.