A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast

The PhyB-PIF light-inducible dimerization system is used to achieve rapid, reversible, and titratable control of protein localization in budding yeast. This system can be used to dynamically activate or inactivate proteins of interest. It is used here to probe where and when Clb2 activity is required for nuclear fission and spindle stabilization.

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