PRODUCTS OF CHLOROPHYLL PHOTODEGRADATION–1. DETECTION and SEPARATION

Abstract— The photodegradative fate of 14C‐radiolabelled phaeophytin a adsorbed on hydrophobic particles in an aqueous suspension is followed. An approximate mass balance is made for the hydrophilic, hydrophobic and CO, fractions. In addition, components of the colourless hydrophilic fraction produced from photodegrading chlorophyll a, phaeophytin a, phaeophorbide a and chlorophyll b are detected and separated using reverse‐phase ion‐pair and ion‐exclusion HPLC with UV detection. The increase in the hydrophilic molecules and the decrease in chlorophyll a are measured as the photodegradation proceeds.

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