A genetic framework for floral patterning

The initial steps of flower development involve two classes of consecutively acting regulatory genes. Meristem-identity genes, which act early to control the initiation of flowers, are expressed throughout the incipient floral primordium. Homeotic genes, which act later to specify the identity of individual floral organs, are expressed in distinct domains within the flower. The link between the two classes of genes has remained unknown so far. Here we show that the meristem-identity gene LEAFY has a role in controlling homeotic genes that is separable from its role in specifying floral fate. On the basis of our observation that LEAFY activates different homeotic genes through distinct mechanisms, we propose a genetic framework for the control of floral patterning.

[1]  L. Guarente,et al.  Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site , 1983, Cell.

[2]  S. McKnight,et al.  Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression. , 1988, Genes & development.

[3]  R. B. Greaves,et al.  The C‐terminal 79 amino acids of the herpes simplex virus regulatory protein, Vmw65, efficiently activate transcription in yeast and mammalian cells in chimeric DNA‐binding proteins. , 1989, The EMBO journal.

[4]  E. Coen,et al.  floricaula: A homeotic gene required for flower development in antirrhinum majus , 1990, Cell.

[5]  J. Bowman,et al.  Negative regulation of the Arabidopsis homeotic gene AGAMOUS by the APETALA2 product , 1991, Cell.

[6]  W. D. Cress,et al.  Critical structural elements of the VP16 transcriptional activation domain. , 1991, Science.

[7]  G. Haughn,et al.  LEAFY, a Homeotic Gene That Regulates Inflorescence Development in Arabidopsis. , 1991, The Plant cell.

[8]  E. Coen,et al.  The war of the whorls: genetic interactions controlling flower development , 1991, Nature.

[9]  J. Bowman,et al.  Genetic interactions among floral homeotic genes of Arabidopsis. , 1991, Development.

[10]  D. Weigel,et al.  LEAFY controls floral meristem identity in Arabidopsis , 1992, Cell.

[11]  Cindy Gustafson-Brown,et al.  Molecular characterization of the Arabidopsis floral homeotic gene APETALA1 , 1992, Nature.

[12]  Hong Ma,et al.  Ectopic expression of the floral homeotic gene AGAMOUS in transgenic Arabidopsis plants alters floral organ identity , 1992, Cell.

[13]  Elliot M. Meyerowitz,et al.  The homeotic gene APETALA3 of Arabidopsis thaliana encodes a MADS box and is expressed in petals and stamens , 1992, Cell.

[14]  I. Sussex,et al.  LEAFY Interacts with Floral Homeotic Genes to Regulate Arabidopsis Floral Development. , 1992, The Plant cell.

[15]  D Weigel,et al.  Activation of Floral Homeotic Genes in Arabidopsis , 1993, Science.

[16]  J. Ellis,et al.  In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants , 1993 .

[17]  R. Müller,et al.  Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. , 1994, Nucleic acids research.

[18]  Elliot M. Meyerowitz,et al.  The ABCs of floral homeotic genes , 1994, Cell.

[19]  M. Van Montagu,et al.  Control of Arabidopsis flower and seed development by the homeotic gene APETALA2. , 1994, The Plant cell.

[20]  S. Hake,et al.  Expression of maize KNOTTED1 related homeobox genes in the shoot apical meristem predicts patterns of morphogenesis in the vegetative shoot , 1994 .

[21]  E. Meyerowitz,et al.  Arabidopsis homeotic gene APETALA3 ectopic expression: Transcriptional and posttranscriptional regulation determine floral organ identity , 1994, Cell.

[22]  Cindy Gustafson-Brown,et al.  Regulation of the arabidopsis floral homeotic gene APETALA1 , 1994, Cell.

[23]  A. Willems,et al.  Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure , 1995, Yeast.

[24]  E. Coen,et al.  Expression of floricaula in single cell layers of periclinal chimeras activates downstream homeotic genes in all layers of floral meristems. , 1995, Development.

[25]  E M Meyerowitz,et al.  LEUNIG regulates AGAMOUS expression in Arabidopsis flowers. , 1995, Development.

[26]  M. Yanofsky.  Floral Meristems to Floral Organs: Genes Controlling Early Events in Arabidopsis Flower Development , 1995 .

[27]  J. Levin,et al.  UFO: an Arabidopsis gene involved in both floral meristem and floral organ development. , 1995, The Plant cell.

[28]  Detlef Weigel,et al.  A developmental switch sufficient for flower initiation in diverse plants , 1995, Nature.

[29]  G. Haughn,et al.  UNUSUAL FLORAL ORGANS Controls Meristem Identity and Organ Primordia Fate in Arabidopsis. , 1995, The Plant cell.

[30]  E. Meyerowitz,et al.  The Arabidopsis homeotic genes APETALA3 and PISTILLATA are sufficient to provide the B class organ identity function. , 1996, Development.

[31]  June I. Medford,et al.  A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis , 1996, Nature.

[32]  J. Mol,et al.  The No Apical Meristem Gene of Petunia Is Required for Pattern Formation in Embryos and Flowers and Is Expressed at Meristem and Primordia Boundaries , 1996, Cell.

[33]  G. Jiménez,et al.  In vivo interactions of the Drosophila Hairy and Runt transcriptional repressors with target promoters. , 1996, The EMBO journal.

[34]  G. Ruvkun,et al.  VP16-activation of the C. elegans neural specification transcription factor UNC-86 suppresses mutations in downstream genes and causes defects in neural migration and axon outgrowth. , 1997, Development.

[35]  R. Simon,et al.  Dual role for fimbriata in regulating floral homeotic genes and cell division in Antirrhinum , 1997, The EMBO journal.

[36]  D. Weigel,et al.  Floral determination and expression of floral regulatory genes in Arabidopsis. , 1997, Development.

[37]  Mike Tyers,et al.  F-Box Proteins Are Receptors that Recruit Phosphorylated Substrates to the SCF Ubiquitin-Ligase Complex , 1997, Cell.

[38]  E. Meyerowitz,et al.  A Polycomb-group gene regulates homeotic gene expression in Arabidopsis , 1997, Nature.

[39]  R. Deshaies,et al.  A Complex of Cdc4p, Skp1p, and Cdc53p/Cullin Catalyzes Ubiquitination of the Phosphorylated CDK Inhibitor Sic1p , 1997, Cell.

[40]  Detlef Weigel,et al.  A LEAFY co-regulator encoded by UNUSUAL FLORAL ORGANS , 1997, Current Biology.

[41]  L. Sieburth,et al.  Molecular dissection of the AGAMOUS control region shows that cis elements for spatial regulation are located intragenically. , 1997, The Plant cell.

[42]  D Weigel,et al.  LEAFY expression and flower initiation in Arabidopsis. , 1997, Development.

[43]  Robert W. Williams,et al.  The CLAVATA1 Gene Encodes a Putative Receptor Kinase That Controls Shoot and Floral Meristem Size in Arabidopsis , 1997, Cell.

[44]  A. Kumar,et al.  An additional role for the F-box motif: gene regulation within the Neurospora crassa sulfur control network. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[45]  M. Tyers,et al.  Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box proteincomplexes that regulate cell division and methionine biosynthesis in yeast. , 1998, Genes & development.

[46]  D Weigel,et al.  Flowering-time genes modulate the response to LEAFY activity. , 1998, Genetics.