Dental wear and oral pathology among sex determined Early Bronze-Age children from Franzhausen I, Lower Austria

The physical properties of diet and oral health throughout childhood play an important role in the development of human dentition, and differed greatly before the industrial revolution. In this study we examined dental wear and oral pathology in a sample of children from the Early Bronze-Age to investigate the physical and mechanical properties of childhood diet and related oral health. We explore cross-sectional age and sex-based variation of children in the sample. The analysis was carried out on the dentitions of 75 children, 978 teeth, excavated from the Early Bronze-Age cemetery Franzhausen I in Lower Austria. Presence of dental caries and calculus was recorded. Dental wear was measured using dentine exposure, occlusal topography, and dental microwear texture analysis. Sex determination was carried out using amelogenin peptide analysis. Caries were found in only 4 individuals (crude prevalence rate—5%, 95% CI 1% to 13%), affecting only 5 teeth (true prevalence rate—less than 1%). Dentine exposure was observed in over 70% of deciduous molars and dental wear measurements indicate a comparatively strong dental wear accumulation especially, among younger children, when compared to modern-day and later pre-industrial populations. Microwear textures presented a high complexity (Asfc > 2)/low anisotropy (epLsar < 1) profile, especially in older children. Differences between male and female children were not generally significant but increased dentine exposure was observed in the lower molars of younger female children. Our results suggest that the Early Bronze-Age children at Franzhausen I consumed a non-cariogenic diet, more abrasive and inclusive of harder/polyhedral foodstuffs than present-day children and some later Medieval children. Differences in dental wear accumulation were observed between children within the population, but with minimal variation between the sexes mostly occurring among younger children.

[1]  R. Salisbury,et al.  Gendered burial practices of early Bronze Age children align with peptide-based sex identification: A case study from Franzhausen I, Austria , 2022, Journal of Archaeological Science.

[2]  K. Kowarik,et al.  Food and farming beyond the Alpine lake zone: the archaeobotany of the Copper Age settlements of Lenzing-Burgstall and Ansfelden-Burgwiese in Upper Austria, and an early occurrence of Triticum spelta (spelt) , 2021, Vegetation History and Archaeobotany.

[3]  P. Heimel,et al.  Quantification of dental macrowear using 3D occlusal surface topographic measurements in deciduous and permanent molars of children , 2021, American journal of physical anthropology.

[4]  N. A. Stewart,et al.  Sex estimation of teeth at different developmental stages using dimorphic enamel peptide analysis. , 2021, American journal of physical anthropology.

[5]  S. Benazzi,et al.  Sex-related morbidity and mortality in non-adult individuals from the Early Medieval site of Valdaro (Italy): the contribution of dental enamel peptide analysis , 2020, Journal of Archaeological Science: Reports.

[6]  R. Salisbury,et al.  Child murder in the Early Bronze Age: proteomic sex identification of a cold case from Schleinbach, Austria , 2020, Archaeological and Anthropological Sciences.

[7]  P. Bayle,et al.  Methodological implications of intra- and inter-facet microwear texture variation for human childhood paleo-dietary reconstruction: Insights from the deciduous molars of extant and medieval children from France , 2020, Journal of Archaeological Science: Reports.

[8]  M. Lozano,et al.  Exploring the utility of optical microscopy versus scanning electron microscopy for the quantification of dental microwear , 2020 .

[9]  Halszka Glowacka,et al.  The ontogeny of maximum bite force in humans , 2020, Journal of anatomy.

[10]  Fernando Racimo,et al.  The dental proteome of Homo antecessor , 2020, Nature.

[11]  Melanie J. Miller,et al.  Raising girls and boys in early China: Stable isotope data reveal sex differences in weaning and childhood diets during the eastern Zhou era , 2020, American journal of physical anthropology.

[12]  M. Lozano,et al.  Inferring childhood dietary maturation using buccal and occlusal deciduous molar microwear: a case study from the recent prehistory of the Iberian Peninsula , 2020, Archaeological and Anthropological Sciences.

[13]  N. Herrmann,et al.  Dental microwear texture analysis of Homo sapiens sapiens: Foragers, farmers, and pastoralists. , 2019, American journal of physical anthropology.

[14]  David M. Rocke,et al.  Sex estimation using sexually dimorphic amelogenin protein fragments in human enamel , 2019, Journal of Archaeological Science.

[15]  D. Strait,et al.  Evidence that metallic proxies are unsuitable for assessing the mechanics of microwear formation and a new theory of the meaning of microwear , 2018, Royal Society Open Science.

[16]  N. Yanko,et al.  Frequency of dental caries in children in the Early Iron Age and the Medieval populations from Ukraine , 2017 .

[17]  N. A. Stewart,et al.  Sex determination of human remains from peptides in tooth enamel , 2017, Proceedings of the National Academy of Sciences.

[18]  S. Halcrow,et al.  Investigating weaning using dental microwear analysis: A review , 2017 .

[19]  P. Keyes,et al.  Dental Calculus Arrest of Dental Caries , 2016, Journal of oral biology.

[20]  P. Mahoney,et al.  Deciduous enamel 3D microwear texture analysis as an indicator of childhood diet in medieval Canterbury, England , 2016 .

[21]  J. Mckinley,et al.  Distinguishing dietary indicators of pastoralists and agriculturists via dental microwear texture analysis , 2015 .

[22]  B. Willershausen,et al.  Epidemiological investigation of caries prevalence in first grade school children in Rhineland-Palatinate, Germany , 2015, Head & Face Medicine.

[23]  S. Mays,et al.  Wear on the deciduous molars in a Mediaeval English human population: a study using crown height , 2014 .

[24]  A. Malgosa,et al.  Study of dental occlusion in ancient human remains: a methodological approach. , 2014, Collegium antropologicum.

[25]  J. Eerkens,et al.  Sex-biased weaning and early childhood diet among middle holocene hunter-gatherers in Central California. , 2013, American journal of physical anthropology.

[26]  P. Mahoney.  Testing functional and morphological interpretations of enamel thickness along the deciduous tooth row in human children. , 2013, American journal of physical anthropology.

[27]  L. Bartosiewicz.  Animals in Bronze Age Europe , 2013 .

[28]  K. Brown,et al.  Exploring the relationship between dental wear and status in late medieval subadults from England. , 2013, American journal of physical anthropology.

[29]  Johannes E. Schindelin,et al.  Fiji: an open-source platform for biological-image analysis , 2012, Nature Methods.

[30]  S. Eggers,et al.  1 Caries Through Time : An Anthropological Overview , 2012 .

[31]  B. Maureille,et al.  Human tooth wear in the past and the present: tribological mechanisms, scoring systems, dental and skeletal compensations. , 2012, Archives of oral biology.

[32]  I. Reiche,et al.  Microbial attack of archaeological bones versus high concentrations of heavy metals in the burial environment. A case study of animal bones from a mediaeval copper workshop in Paris , 2011 .

[33]  F. Xue,et al.  Genes, genetics, and Class III malocclusion. , 2010, Orthodontics & craniofacial research.

[34]  E. Defraia,et al.  Dental arch dimensions and tooth wear in two samples of children in the 1950s and 1990s , 2009, BDJ.

[35]  D. Donlon,et al.  Patterns in dental enamel hypoplasia by sex and age at death in two archaeological populations. , 2009, Archives of oral biology.

[36]  C. Deter.  Gradients of occlusal wear in hunter-gatherers and agriculturalists. , 2009, American journal of physical anthropology.

[37]  İ. Arı,et al.  Prevalence of dental caries and tooth wear in a Byzantine population (13th c. A.D.) from northwest Turkey. , 2007, Archives of oral biology.

[38]  J. Kaidonis.  Tooth wear: the view of the anthropologist , 2007, Clinical Oral Investigations.

[39]  Peter S Ungar,et al.  Quantification of dental microwear by tandem scanning confocal microscopy and scale-sensitive fractal analyses. , 2006, Scanning.

[40]  Peter S Ungar,et al.  Dental microwear texture analysis: technical considerations. , 2006, Journal of human evolution.

[41]  Christina Deter,et al.  Dental wear patterns of hunter-gatherers and agriculturalists : the impact of behavioural changes accompanying the transition , 2006 .

[42]  E. Huntington,et al.  Evidence for putting the calculus: caries inverse relationship to work. , 2005, Community dentistry and oral epidemiology.

[43]  D. Guatelli‐Steinberg,et al.  Prevalence and the duration of linear enamel hypoplasia: a comparative study of Neandertals and Inuit foragers. , 2004, Journal of human evolution.

[44]  W. Rock,et al.  The prevalence of tooth erosion in 12-year-old children , 2004, British Dental Journal.

[45]  P. Goel,et al.  Prevalence of dental disease amongst 5-6 and 12-13 year old school children of Puttur municipality, Karnataka State-India. , 2000, Journal of the Indian Society of Pedodontics and Preventive Dentistry.

[46]  L. Wood.  Frequency and chronological distribution of linear enamel hypoplasia in a North American colonial skeletal sample. , 1996, American journal of physical anthropology.

[47]  M. Lennon,et al.  The prevalence of tooth wear in 14-year-old school children in Liverpool. , 1994, Community dental health.

[48]  M. Teschler-Nicola.  [Sexual dimorphism of tooth crown diameters. A contribution to the determination of the sex of subadult individuals from the early bronze age graveyard of Franzhausen I, lower Austria]. , 1992, Anthropologischer Anzeiger; Bericht uber die biologisch-anthropologische Literatur.

[49]  R. Corruccini.  Australian aboriginal tooth succession, interproximal attrition, and Begg's theory. , 1990, American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics.

[50]  G. Maat,et al.  The caries-attrition competition , 1987 .

[51]  S Molnar,et al.  Human tooth wear, tooth function and cultural variability. , 1971, American journal of physical anthropology.

[52]  Dental Wear in Evolutionary and Biocultural Contexts , 2020 .

[53]  J. Watson,et al.  An introduction to dental wear in evolutionary and biocultural contexts , 2020 .

[54]  R. D’Anastasio,et al.  Dental microwear texture analysis in deciduous teeth , 2020 .

[55]  Se-Yeon Kim,et al.  Elucidating the relationship of gingivitis and dental calculus with the periodontal health of 12-year-old children , 2019, Journal of Korean Academy of Oral Health.

[56]  K. Rebay-Salisbury,et al.  Breast is best – and are there alternatives? Feeding babies and young children in prehistoric Europe , 2019 .

[57]  Sebastian Muller.  Human Skeletal Remains Excavation Analysis Interpretation , 2016 .

[58]  P. Velemínský,et al.  The prevalence and distribution of dental caries in four early medieval non-adult populations of different socioeconomic status from Central Europe. , 2015, Archives of oral biology.

[59]  R Core Team,et al.  R: A language and environment for statistical computing. , 2014 .

[60]  M. Zalewska,et al.  Frequency and chronological distribution of linear enamel hypoplasia (LEH) in the Late Neolithic and Early Bronze Age population from Żerniki Górne (Poland) – preliminary report , 2012 .

[61]  Paolo Cignoni,et al.  MeshLab: an Open-Source Mesh Processing Tool , 2008, Eurographics Italian Chapter Conference.

[62]  J. Watson.  Changes in food processing and occlusal dental wear during the early agricultural period in northwest Mexico. , 2008, American journal of physical anthropology.

[63]  G. Truin,et al.  The prevalence of dental caries in Europe 1990-1995 , 1996 .

[64]  T. Varrela.  Prevalence and distribution of dental caries in a late medieval population in Finland. , 1991, Archives of oral biology.

[65]  J. Bullington,et al.  Deciduous dental microwear of prehistoric juveniles from the lower Illinois River valley. , 1991, American journal of physical anthropology.

[66]  B. Smith,et al.  Patterns of molar wear in hunger-gatherers and agriculturalists. , 1984, American journal of physical anthropology.

[67]  T. Davies.,et al.  The degree of attrition of the deciduous teeth and first permanent molars of primitive and urbanised Greenland natives. , 1955 .