The Introduction of Pituitary Gland Extract of Crab-eater Frog (Fejervarya Cancrivora) to Accelerate Ovulation of Eggs and Spawning of Common Carp (Cyprinus Carpio). J. Life Sci. Biomed. 5(5): 153-158

Induced breeding by hypophysation technique is widely known as an effort conducted to stimulate ripe fish breeders to induce ovulation of eggs and spawning through injection of pituitary gland. This research was aimed to determine the most effective dosage of frog (F. cancrivora) pituitary gland which best accelerates ovulation of eggs and spawning of common carp fish (C. carpio). The experimental method was performed with 4 different doses treatment levels (0, 0.3, 0.5, and 0.7 ml/kg) per female brood fish with 3 replications. All data collected from the research was then statistically analyzed using Completely Randomized Design (CRD) method. This research indicated that the most effective dosage was T3 treatment with a dose of 0.5 ml/kg of frogs’ pituitary gland per female brood fish. This treatment showed the highest increase of estradiol-17ß concentration (25253.65 ± 1050.68) pg/ml, and the fastest spawning period (12.03 ± 0.44) hours. The result proves that pituitary gland of crab-eater frog (F. cancrivora) can be used for induced breeding of common carps (C. carpio) with competitive performance compare to other pituitaries.