Spectral characterization of biological aerosol particles using two-wavelength excited laser-induced fluorescence and elastic scattering measurements.

A two-wavelength laser-induced fluorescence (LIF) instrument has been developed and used to characterize individual biological aerosol particles, including biological warfare (BW) agent surrogates. Fluorescence in discrete spectral bands from widely different species, and also from similar species under different growth conditions were measured and compared. The two-wavelength excitation approach was found to increase discrimination among several biological materials, and especially with respect to diesel exhaust particles, a common interferent for LIF BW detection systems. The spectral characteristics of a variety of biological materials and ambient air components have been studied as a function of aerosol particle size and incident fluence.

[1]  E Hirst,et al.  Simultaneous light scattering and intrinsic fluorescence measurement for the classification of airborne particles. , 2000, Applied optics.

[2]  Vasanthi Sivaprakasam,et al.  Multiple UV wavelength excitation and fluorescence of bioaerosols , 2004, SPIE Optics East.

[3]  Jun Q. Lu,et al.  Determination of complex refractive index of polystyrene microspheres from 370 to 1610 nm , 2003, Physics in medicine and biology.

[4]  Yong-Le Pan,et al.  Single-Particle Fluorescence Spectrometer for Ambient Aerosols , 2003 .

[5]  J. E. Tucker,et al.  Classification and selective collection of individual aerosol particles using laser-induced fluorescence. , 2008, Applied optics.

[6]  Vadim A. Markel,et al.  Geometrical renormalization approach to calculating optical properties of fractal carbonaceous soot. , 2001, Journal of the Optical Society of America. A, Optics, image science, and vision.

[7]  Yong-Le Pan,et al.  Dynamics of photon-induced degradation and fluorescence in riboflavin microparticles , 2001 .

[8]  Yoon-Kyu Song,et al.  290 and 340 nm UV LED arrays for fluorescence detection from single airborne particles. , 2005, Optics express.

[9]  N. R. Newbury,et al.  Bio-aerosol fluorescence sensor , 1999 .

[10]  E Hirst,et al.  Single particle multichannel bio-aerosol fluorescence sensor. , 2005, Optics express.

[11]  S. C. Hill,et al.  Single-shot fluorescence spectra of individual micrometer-sized bioaerosols illuminated by a 351- or a 266-nm ultraviolet laser. , 1999, Optics letters.

[12]  Mark Seaver,et al.  Size and Fluorescence Measurements for Field Detection of Biological Aerosols , 1999 .

[13]  K. Mortelmans,et al.  Spectrally resolved absolute fluorescence cross sections for bacillus spores. , 1997, Applied optics.

[14]  Yong-Le Pan,et al.  A Puff of Air Sorts Bioaerosols for Pathogen Identification , 2004 .

[15]  Charles D. Litton,et al.  Studies of the measurement of respirable coal dusts and diesel particulate matter , 2002 .

[16]  John G. Bruno,et al.  Fluorescence Particle Counter for Detecting Airborne Bacteria and Other Biological Particles , 1995 .

[17]  I. Malitson.  Interspecimen Comparison of the Refractive Index of Fused Silica , 1965 .

[18]  Jay D. Eversole,et al.  Field test results and ambient aerosol measurements using dual wavelength fluorescence excitation and elastic scatter for bio-aerosols , 2007, SPIE Defense + Commercial Sensing.

[19]  J. Ho,et al.  Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence. , 1997, Journal of aerosol science.