Enzyme catalysis captured using multiple structures from one crystal at varying temperatures

MSOX (multiple serial structures from one crystal) serial crystallography experiments were carried out using controlled X-ray radiolysis and photon-counting detectors to determine sequences of low-dose yet high-resolution structures of copper nitrite reductase. Working at 190 K and at room temperature provides greater dynamic freedom, allowing more of the catalytic cycle to be observed than at the usual cryogenic temperature of 100 K. The approach demonstrates the potential to obtain MSOX structural movies at variable temperatures, thus providing an unparalleled level of structural information during catalysis for redox enzymes.

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