Evaluation of anesthesia effects on [18F]FDG uptake in mouse brain and heart using small animal PET.

This study evaluates effects of anesthesia on (18)F-FDG (FDG) uptake in mouse brain and heart to establish the basic conditions of small animal PET imaging. Prior to FDG injection, 12 mice were anesthetized with isoflurane gas; 11 mice were anesthetized with an intraperitoneal injection of a ketamine/xylazine mixture; and 11 mice were awake. In isoflurane and ketamine/xylazine conditions, FDG brain uptake (%ID/g) was significantly lower than in controls. Conversely, in the isoflurane condition, %ID/g in heart was significantly higher than in controls, whereas heart uptake in ketamine/xylazine mice was significantly lower. Results suggest that anesthesia impedes FDG uptake in mouse brain and affects FDG uptake in heart; however, the effects in the brain and heart differ depending on the type of anesthesia used.

[1]  Jeih-San Liow,et al.  A single slice rebinning/2D exact positioning OSEM reconstruction for the NIH ATLAS small animal PET scanner , 2003 .

[2]  Jurgen Seidel,et al.  Resolution uniformity and sensitivity of the NIH ATLAS small animal PET scanner: comparison to simulated LSO scanners without depth-of-interaction capability , 2001 .

[3]  C. Porro,et al.  Ketamine Effects on Local Cerebral Blood Flow and Metabolism in the Rat , 1987, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[4]  D A Benaron,et al.  Imaging transgenic animals. , 1999, Annual review of biomedical engineering.

[5]  K. L. Smiler,et al.  Anesthesia and Analgesia in Rodents , 1997 .

[6]  A. Verkman,et al.  Analysis of organ physiology in transgenic mice. , 2000, American journal of physiology. Cell physiology.

[7]  M E Easterly,et al.  A review of high-resolution X-ray computed tomography and other imaging modalities for small animal research. , 2001, Lab animal.

[8]  W C Eckelman,et al.  High resolution PET, SPECT and projection imaging in small animals. , 2001, Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society.

[9]  J. Knight,et al.  Ketamine alone and combined with diazepam or xylazine in laboratory animals: a 10 year experience , 1981, Laboratory animals.

[10]  L. Sokoloff,et al.  Local glucose utilization and local blood flow in hearts of awake rats , 1993, Basic Research in Cardiology.

[11]  K van Ackern,et al.  Local coupling of cerebral blood flow to cerebral glucose metabolism during inhalational anesthesia in rats: desflurane versus isoflurane. , 1999, Anesthesiology.

[12]  L. Sokoloff,et al.  Local Cerebral Glucose Utilization in Controlled Graded Levels of Hyperglycemia in the Conscious Rat , 1988, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[13]  S. Nelson,et al.  Ketamine-induced changes in regional glucose utilization in the rat brain. , 1980, Anesthesiology.

[14]  Arion F. Chatziioannou,et al.  Molecular imaging of small animals with dedicated PET tomographs , 2001, European Journal of Nuclear Medicine and Molecular Imaging.

[15]  S S Gambhir,et al.  Use of positron emission tomography in animal research. , 2001, ILAR journal.

[16]  Sanjiv S Gambhir,et al.  Indirect monitoring of endogenous gene expression by positron emission tomography (PET) imaging of reporter gene expression in transgenic mice. , 2002, Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging.

[17]  Z. Bosnjak,et al.  Anesthetic Effects on Mitochondrial ATP-sensitive K Channel , 2001, Anesthesiology.

[18]  M. Reivich,et al.  THE [14C]DEOXYGLUCOSE METHOD FOR THE MEASUREMENT OF LOCAL CEREBRAL GLUCOSE UTILIZATION: THEORY, PROCEDURE, AND NORMAL VALUES IN THE CONSCIOUS AND ANESTHETIZED ALBINO RAT 1 , 1977, Journal of neurochemistry.

[19]  A. Saija,et al.  Modifications of the permeability of the blood-brain barrier and local cerebral metabolism in pentobarbital- and ketamine-anaesthetized rats , 1989, Neuropharmacology.

[20]  L. Sokoloff,et al.  Effects of diazepam and ketamine administered individually or in combination on regional rates of glucose utilization in rat brain. , 1999, British journal of anaesthesia.

[21]  Dennis F. Kohn,et al.  Anesthesia and analgesia in laboratory animals , 2008 .

[22]  M E Phelps,et al.  Positron emission tomography provides molecular imaging of biological processes. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[23]  S R Cherry,et al.  Quantitative Assessment of Longitudinal Metabolic Changes In Vivo after Traumatic Brain Injury in the Adult Rat using FDG-MicroPET , 2000, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[24]  Matthew R. Cohen,et al.  Isoflurane Alters Energy Substrate Metabolism to Preserve Mechanical Function in Isolated Rat Hearts following Prolonged No-Flow Hypothermic Storage , 2003, Anesthesiology.

[25]  G. Sharp,et al.  Inhibition of glucose-induced insulin release by xylazine. , 1985, Endocrinology.

[26]  I. Anand,et al.  Ischemic preconditioning prior to aortic cross-clamping protects high-energy phosphate levels, glucose uptake, and myocyte contractility. , 2002, The Journal of surgical research.

[27]  C Ori,et al.  Effects of Isoflurane Anesthesia on Local Cerebral Glucose Utilization in the Rat , 1986, Anesthesiology.

[28]  K van Ackern,et al.  Local Cerebral Blood Flow, Local Cerebral Glucose Utilization, and Flow‐Metabolism Coupling during Sevoflurane versus Isoflurane Anesthesia in Rats , 1998, Anesthesiology.