A comparative study on the porins of Rhodobacter capsulatus strains 37b4 and B10 grown under different culture conditions

Summary The cell wall protein composition and the isolated porins of Rhodobacter capsulatus strains 37b4 and B10 were comparatively studied. The porins from the two strains migrated differently on SDS-PAGE in the absence (oligomers; Mr 92 000 and 89 000, monomers; Mr 44 000 and 32 000, for strains 37b4 and B10, respectively) and in the presence of 8 M urea. N-terminal sequencing of the two purified porins demonstrated that the sequences were different in five positions out of 21 positions determined. With both strains, the cell walls showed no growth condition dependent differences in their protein patterns. In both cases, only the porin given above was expressed in photoorganoheterotrophically (organotrophic) and photolithoautotrophically (lithotrophic) grown cells. There was also no difference between cells grown with glucose instead of malic acid or even using cells grown under phosphate starvation. In lipid bilayer experiments the isolated porins from Rhodobacter capsulatus 37b4 and B10 had virtually the same single-channel properties. Furthermore, the porins obtained from cells grown under the given various different culture conditions revealed the same single-channel conductance. In all cases a slight cation-selectivity was observed.

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