Gametocytogenesis of Leucocytozoon caulleryi in In Vitro Culture: Effect of Human Red Blood Cells on the Development of Second‐Generation Merozoites

For investigating development of second‐generation merozoites of Leucocytozoon caulleryi into mature gametocytes, infected erythrocytes from chickens at 15 d after sporozoites inoculation were cultured in RPMI‐1640 modified medium supplemented with 10% horse serum and 0.5 ml of human erythrocytes (type O). When culture was carried out at 37°C in a humidified atmosphere of 5% CO2 for 7 d, the very small number of second‐generation merozoites developed into morphologically mature gametocytes. However, in the high carbon dioxide and low oxygen condition, mature gametocytes weren't observed in cluture. The role of human erythrocytes added has not been clarified yet.

[1]  T. Nash,et al.  Isoprenylation of proteins in the protozoan Giardia lamblia. , 1995, Molecular and biochemical parasitology.

[2]  A. Mendez.  Monensin and Brefeldin A Inhibit High Density Lipoprotein- mediated Cholesterol Efflux from Cholesterol-enriched Cells , 1995, The Journal of Biological Chemistry.

[3]  T. Nash,et al.  Serum Cohn fraction IV-1 supports the growth of Giardia lamblia in vitro , 1994, Infection and immunity.

[4]  L. Helman,et al.  Insulin-like growth factors stimulate growth and L-cysteine uptake by the intestinal parasite Giardia lamblia. , 1994, Journal of Biological Chemistry.

[5]  M. Fukuda,et al.  Observations on First-Generation Schizogony of Leucocytozoon caulleryi in Chickens , 1992 .

[6]  J. Bruce,et al.  Giardia lamblia: phospholipid analysis of human isolates. , 1991, Annals of tropical medicine and parasitology.

[7]  N. Fidge,et al.  Identification of a multispecific lipoprotein receptor in adult Schistosoma japonicum by ligand blotting analyses. , 1989, Molecular and biochemical parasitology.

[8]  T. Iijima,et al.  Cryopreservation of Leucocytozoon caulleryi sporozoites. , 1988, The Journal of protozoology.

[9]  M. Pereira,et al.  Specific binding of human plasma high density lipoprotein (cruzin) to Trypanosoma cruzi. , 1988, Molecular and biochemical parasitology.

[10]  K. Nakamura,et al.  Observations on the Taiwanese strain of Leucocytozoon caulleryi (Haemosporina) in chickens. , 1986, The Journal of protozoology.

[11]  J. Alderete,et al.  Trichomonas vaginalis is dependent on uptake and degradation of human low density lipoproteins , 1984, Journal of Experimental Medicine.

[12]  P. Mukerjee,et al.  Bile Salts as Atypical Surfactants and Solubilizers , 1984, Hepatology.

[13]  T. Iijima,et al.  Infectivity of Leucocytozoon caulleryi sporozoites developed in vitro and in vivo. , 1984, International journal for parasitology.

[14]  T. Imada,et al.  Development of second generation merozoites of Leucocytozoon caulleryi in vitro. , 1984, The Journal of protozoology.

[15]  D. S. Reiner,et al.  Mass cultivation of Giardia lamblia in a serum-free medium. , 1983, Journal of Parasitology.

[16]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[17]  M. Rogers.  Do parasites express receptors for host lipoproteins? , 1991, Parasitology today.

[18]  K. Fujisaki,et al.  Rapid detection by counterimmunoelectrophoresis of antigens and antibodies in the sera of chickens infected with Leucocytozoon caulleryi. , 1980, National Institute of Animal Health quarterly.

[19]  S. Inui,et al.  Morphology and distribution of intracellular schizonts in chickens experimentally infected with Akiba caulleryi. , 1971, National Institute of Animal Health quarterly.

[20]  K. Akiba.  Leucocytozoonosis of chickens. , 1970 .

[21]  T. Morii,et al.  The laboratory colonization of Culicoides arakwae (Diptera. Ceratopogonidae). , 1968, National Institute of Animal Health quarterly.