O-アセチル転移酵素高産生株を用いたウムテストおよび Ames 試験による河川水の遺伝毒性モニタリング

Evaluation of genotoxicity of organic extracts from the Yodo River system was examined with umu test and Ames test both using an O-acetyltransferase-overexpressing strain. Organic extracts in river water were collected by the XAD-2 resin column method and the blue rayon hanging method at 4 locations for one year.The same level of genotoxic activity was observed among the presence and the absence of S9 mix in umu test using S. typhimurium NM2009. But, higher activities were observed in the presence of S9 mix in Ames test using S. typhimurium YG1024. These results shows that S. typhimurium YG1024 in Ames test is highly sensitive to aromatic amines in water environment. There were a significant correlation between genotoxic activity induced by NM2009 in umu test and one by YG1024 in Ames test in the presence of S9 mix. No significant correlation was found between genotoxicities and water pollution indicators. The present results shows that umu test or Ames test using an O-acetyltransferase-overexpressing strain are simple, sensitive and useful bioassay for monitoring the safety of river water.

[1]  H. Hayatsu,et al.  Evaluation of blue-chitin column, blue-rayon hanging, and XAD-resin column techniques for concentrating mutagens from two Japanese rivers. , 1996, Mutation research.

[2]  Y. Ono,et al.  Genotoxicity of substances in the nightsoil and its biologically treated water , 1996 .

[3]  T. Ohe,et al.  Genotoxic activity of 1-nitropyrene in water from the Yodo River, Japan. , 1996, The Science of the total environment.

[4]  H. Yamazaki,et al.  Development of high sensitive umu test system: rapid detection of genotoxicity of promutagenic aromatic amines by Salmonella typhimurium strain NM2009 possessing high O-acetyltransferase activity. , 1995, Mutation research.

[5]  K. Kawai,et al.  Genotoxic activity in vivo of the naturally occurring glucoside, cycasin, in the Drosophila wing spot test. , 1995, Mutation research.

[6]  T. Sugimura,et al.  Mutagenicities of Bangkok and Tokyo river waters. , 1994, Mutation research.

[7]  紘平 浦野,et al.  水道水のAmes変異原性に関する研究 第1報 変異原性物質濃縮回収用の吸着剤 , 1994 .

[8]  H. Ueno,et al.  Identification of polycyclic aromatic hydrocarbons in mutagenic adsorbates to a copper-phthalocyanine derivative recovered from municipal river water. , 1993, Mutation research.

[9]  H. Ueno,et al.  Evaluation of Mutagenicity of Municipal River Water Concentrated Using XAD Resin Column Method , 1992 .

[10]  Y. Ono,et al.  Genotoxicity of Organic Substances in Municipal Sewage and Its Ozonated Products , 1992 .

[11]  H. Ueno,et al.  Mutagenicity of adsorbates to a copper-phthalocyanine derivative recovered from municipal river water. , 1990, Mutation research.

[12]  H. Hayatsu,et al.  A simple method for monitoring mutagenicity of river water. Mutagens in Yodo river system, Kyoto-Osaka , 1990, Bulletin of environmental contamination and toxicology.

[13]  H. Shinagawa,et al.  Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens. , 1985, Mutation research.

[14]  B. Ames,et al.  Revised methods for the Salmonella mutagenicity test. , 1983, Mutation research.

[15]  S. Maruoka,et al.  Mutagenicity in Salmonella typhimurium tester strains of XAD-2-ether extract, recovered from Katsura River water in Kyoto City, and its fractions. , 1982, Mutation research.

[16]  A. Nagai,et al.  Mutagenicity of River Water in the Upper Areas of Nagara River. , 1996 .

[17]  Y. Ono,et al.  Chemical and Biological Influence of Hazardous Substances and Obstacle Organisms to Aquatic Environment and Their Control. Risk Assessment of Genotoxic Substances Related to Human Diet in the Water Environment. , 1996 .

[18]  G. V. Calder,et al.  Use of macroreticular resins in the analysis of water for trace organic contaminants. , 1974, Journal of chromatography.