A new Lab-on-Chip transmitter for the detection of proteins using RNA aptamers

A new RNA aptamer based affinity biosensor for C-Reactive Protein (CRP), a risk marker for cardiovascular disease was developed using interdigitated capacitor (IDC), integrated in Voltage Controlled Oscillator (VCO) and output signal is amplified using Single Stage Power Amplifier (PA) for transmitting signal to receiver at Industrial, Scientific and Medical (ISM) band. The Lab-on-Chip transmitter design includes IDC, VCO and PA. The design was implemented in IHP 0.25µm SiGe BiCMOS process; post-CMOS process was utilized to increase the sensitivity of biosensor. The CRP was incubated between or on interdigitated electrodes and the changes in capacitance of IDC occurred. In blank measurements, the oscillation frequency was 2.464GHz whereas after RNA aptamers were immobilized on open aluminum areas of IDC and followed by binding reaction processed with 500pg/ml CRP solution, the capacitance shifted to 2.428GHz. Phase noise is changed from −114.3dBc/Hz to −116.5dBc/Hz.

[1]  M. Kaynak,et al.  Label-free, capacitive immunosensor for protein detection , 2006, 2006 5th IEEE Conference on Sensors.

[2]  J.G. Atallah,et al.  A low phase noise VCO for multi band wireless transceivers , 2007, 2007 International Conference on Design & Technology of Integrated Systems in Nanoscale Era.

[3]  Mengsu Yang,et al.  Adsorption kinetics and ligand-binding properties of thiol-modified double-stranded DNA on a gold surface , 1998 .

[4]  S Tombelli,et al.  Development of an optical RNA-based aptasensor for C-reactive protein , 2008, Analytical and bioanalytical chemistry.

[5]  Gil U. Lee,et al.  Covalent attachment of synthetic DNA to self-assembled monolayer films. , 1996, Nucleic acids research.

[6]  Y. Gurbuz,et al.  A 4.5-5.8 GHz Differential LC VCO using 0.35 μm SiGe BiCMOS Technology , 2006, 2006 European Microwave Integrated Circuits Conference.

[7]  G. Whitesides,et al.  Self-assembled monolayers of thiolates on metals as a form of nanotechnology. , 2005, Chemical reviews.