A systematic review of the influence of different titanium surfaces on proliferation, differentiation and protein synthesis of osteoblast-like MG63 cells.

OBJECTIVES Titanium is the standard material for dental and orthopaedical implants. The good biocompatibility has been proven in many experimental and clinical investigations. Different titanium topographies were tested in vitro using different cell culture models. The aim of this systematic review was to evaluate and summarize the medical/dental literature to assess on which kind of titanium surface structure the osteoblast-like osteosarcoma cells MG63 show the best proliferation and differentiation rate, and the best protein synthesis. METHODS A systematic search was carried out using different on-line databases (PubMed, Web of Science, Cochrane Library, International Poster Journal), supplemented by handsearch in selected journals and by examination of the bibliographies of the identified articles. Inclusion and exclusion criterias were applied when considering relevant articles. Studies which met the inclusion criteria were included and data extraction was undertaken by one reviewer. RESULTS The search yielded 348 references. Nine articles referring to nine different studies were relevant to our question. Additionally 8 less relevant articles were identified. It was found that regularly textured surfaces of pure titanium with R(a) values (average roughness) of around 4 mum are well-accepted by MG63 cells. CONCLUSIONS The surfaces and culture conditions vary widely. Therefore it is still difficult to recommend one particular surface. It seems that there are no differences in cell proliferation and differentiation on surfaces treated by blasting and etching. Standardization in fabrication and size of the different test surfaces as well as homogeneity in culture times and plating densities should be aspects for future research.

[1]  B D Boyan,et al.  Surface roughness modulates the local production of growth factors and cytokines by osteoblast-like MG-63 cells. , 1996, Journal of biomedical materials research.

[2]  L. Bonewald,et al.  Effects of combining transforming growth factor beta and 1,25-dihydroxyvitamin D3 on differentiation of a human osteosarcoma (MG-63). , 1992, The Journal of biological chemistry.

[3]  D. Dean,et al.  Prostaglandins mediate the effects of titanium surface roughness on MG63 osteoblast-like cells and alter cell responsiveness to 1 alpha,25-(OH)2D3. , 1998, Journal of biomedical materials research.

[4]  L. Raisz,et al.  Stimulation of bone formation by prostaglandin E2. , 1984, Prostaglandins.

[5]  D. Campoccia,et al.  In vitro effects on MG63 osteoblast-like cells following contact with two roughness-differing fluorohydroxyapatite-coated titanium alloys. , 2002, Biomaterials.

[6]  B. Kasemo,et al.  Biomaterial and implant surfaces: a surface science approach. , 1988, The International journal of oral & maxillofacial implants.

[7]  C. Lohmann,et al.  Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition. , 1998, Biomaterials.

[8]  H. Nagura,et al.  Effect of a titanium surface on bone marrow-derived osteoblastic cells in vitro. , 1997, Archives of oral biology.

[9]  J. Ong,et al.  SURFACE ROUGHNESS OF TITANIUM ON BONE MORPHOGENETIC PROTEIN‐2 TREATED OSTEOBLAST CELLS IN VITRO , 1997, Implant dentistry.

[10]  W. Nisch,et al.  Variation in contact guidance by human cells on a microstructured surface. , 1995, Journal of biomedical materials research.

[11]  B. Boyan,et al.  Inhibition of cyclooxygenase by indomethacin modulates osteoblast response to titanium surface roughness in a time-dependent manner. , 2001, Clinical oral implants research.

[12]  B. Kasemo.  Biocompatibility of titanium implants: surface science aspects. , 1983, The Journal of prosthetic dentistry.

[13]  H. Larjava,et al.  Hannu Larjava Tissues Extracellular Matrix Molecules and their Receptors : An Overview with Special Emphasis on Periodontal , 1991 .

[14]  A Scarano,et al.  Surface analysis of machined versus sandblasted and acid-etched titanium implants. , 2000, The International journal of oral & maxillofacial implants.

[15]  B. Boyan,et al.  The effects of vitamin D metabolites on phospholipase A2 activity of growth zone and resting zone cartilage cells in vitro. , 1988, Endocrinology.

[16]  Maxence Bigerelle,et al.  The relative influence of the topography and chemistry of TiAl6V4 surfaces on osteoblastic cell behaviour. , 2000, Biomaterials.

[17]  B. Boyan,et al.  Titanium surface roughness alters responsiveness of MG63 osteoblast‐like cells to 1α,25‐(OH)2D3 , 1998 .

[18]  D Buser,et al.  Biologic width around titanium implants. A histometric analysis of the implanto-gingival junction around unloaded and loaded nonsubmerged implants in the canine mandible. , 1997, Journal of periodontology.

[19]  L. Bonewald,et al.  Localization of 1,25-(OH)2D3-responsive alkaline phosphatase in osteoblast-like cells (ROS 17/2.8, MG 63, and MC 3T3) and growth cartilage cells in culture. , 1989, The Journal of biological chemistry.

[20]  G. Gronowicz,et al.  Response of human osteoblasts to implant materials: Integrin‐mediated adhesion , 1996, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[21]  B. Boyan,et al.  Surface roughness mediates its effects on osteoblasts via protein kinase A and phospholipase A2. , 1999, Biomaterials.

[22]  E Milella,et al.  Preparation and characterisation of titania/hydroxyapatite composite coatings obtained by sol-gel process. , 2001, Biomaterials.

[23]  G. Stein,et al.  Progressive changes in the protein composition of the nuclear matrix during rat osteoblast differentiation. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[24]  L. Bonewald,et al.  Maturation State Determines the Response of Osteogenic Cells to Surface Roughness and 1,25‐Dihydroxyvitamin D3 , 2000, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[25]  C. Stanford,et al.  The concept of osseointegration and bone matrix expression. , 1991, Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists.

[26]  L. Raisz,et al.  Biphasic effects of prostaglandin E2 on bone formation in cultured fetal rat calvariae: interaction with cortisol. , 1990, Endocrinology.

[27]  B. Boyan,et al.  Both cyclooxygenase-1 and cyclooxygenase-2 mediate osteoblast response to titanium surface roughness. , 2001, Journal of biomedical materials research.

[28]  U. Hübner,et al.  The titanium surface texture effects adherence and growth of human gingival keratinocytes and human maxillar osteoblast-like cells in vitro. , 2001, Biomaterials.

[29]  R. Tuan,et al.  Enhanced extracellular matrix production and mineralization by osteoblasts cultured on titanium surfaces in vitro. , 1992, Journal of cell science.

[30]  B D Boyan,et al.  Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63). , 1995, Journal of biomedical materials research.

[31]  B. Boyan,et al.  Osteoblast response to titanium surface roughness and 1alpha,25-(OH)(2)D(3) is mediated through the mitogen-activated protein kinase (MAPK) pathway. , 2001, Journal of biomedical materials research.

[32]  P. Ramires,et al.  The influence of titania/hydroxyapatite composite coatings on in vitro osteoblasts behaviour. , 2001, Biomaterials.

[33]  C Hendrich,et al.  [Standardized testing of bone implant surfaces with an osteoblast cell culture system. II. Titanium surfaces of different degrees of roughness]. , 1999, Biomedizinische Technik. Biomedical engineering.

[34]  J. Ong,et al.  Osteoblast progenitor cell responses to characterized titanium surfaces in the presence of bone morphogenetic protein-atelopeptide type I collagen in vitro. , 1999, The Journal of oral implantology.

[35]  L. Cooper,et al.  Formation of mineralizing osteoblast cultures on machined, titanium oxide grit-blasted, and plasma-sprayed titanium surfaces. , 1999, The International journal of oral & maxillofacial implants.

[36]  M. Yamazaki,et al.  Prostaglandin E2 Stimulates Bone Sialoprotein (BSP) Expression through cAMP and Fibroblast Growth Factor 2 Response Elements in the Proximal Promoter of the Rat BSP Gene* , 2003, Journal of Biological Chemistry.

[37]  G. Stein,et al.  Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix , 1990, Journal of cellular physiology.

[38]  J. Wergedal,et al.  Human bone cell phenotypes differ depending on their skeletal site of origin. , 1995, The Journal of clinical endocrinology and metabolism.

[39]  D. Pioletti,et al.  Effect of different Ti-6Al-4V surface treatments on osteoblasts behaviour. , 2002, Biomaterials.

[40]  B. Boyan,et al.  Proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63) cultured on previously used titanium surfaces. , 1996, Clinical oral implants research.

[41]  R. Thull,et al.  Der Effekt von Titanoberflächen unterschiedlicher Rauhigkeit auf Zellproliferation, Zelldifferenzierung und Proteinsynthese humaner fetaler Osteoblasten (hFOB 1.19) , 1997 .

[42]  T Albrektsson,et al.  The electrochemical oxide growth behaviour on titanium in acid and alkaline electrolytes. , 2001, Medical engineering & physics.

[43]  M. Gowen,et al.  Are MG-63 and HOS TE85 human osteosarcoma cell lines representative models of the osteoblastic phenotype? , 1994, Bone.

[44]  P. Descouts,et al.  Osteoblast culture on polished titanium disks modified with phosphonic acids. , 2002, Journal of biomedical materials research.

[45]  A Wennerberg,et al.  Determining optimal surface roughness of TiO(2) blasted titanium implant material for attachment, proliferation and differentiation of cells derived from human mandibular alveolar bone. , 2001, Clinical oral implants research.

[46]  L. Bonewald,et al.  Stimulation of matrix vesicle enzyme activity in osteoblast-like cells by 1,25(OH)2D3 and transforming growth factor β (TGFβ) , 1992 .

[47]  M E Bolander,et al.  Transforming growth factor-beta and the initiation of chondrogenesis and osteogenesis in the rat femur , 1990, The Journal of cell biology.

[48]  U. Senin,et al.  Cathepsin B activity in normal human osteoblast-like cells and human osteoblastic osteosarcoma cells (MG-63): regulation by interleukin-1 beta and parathyroid hormone. , 1996, Biochimica et biophysica acta.

[49]  B. Nebe,et al.  Cell-extracellular matrix interaction and physico-chemical characteristics of titanium surfaces depend on the roughness of the material. , 2002, Biomolecular engineering.

[50]  B. Boyan,et al.  Shear force modulates osteoblast response to surface roughness. , 2002, Journal of biomedical materials research.

[51]  M. McKee,et al.  Chemical modification of titanium surfaces for covalent attachment of biological molecules. , 1998, Journal of biomedical materials research.

[52]  B. Boyan,et al.  Regulation of prostaglandin E2 production by vitamin D metabolites in growth zone and resting zone chondrocyte cultures is dependent on cell maturation. , 1992, Bone.

[53]  P. Branemark,et al.  Osseointegrated titanium fixtures in the treatment of edentulousness. , 1983, Biomaterials.

[54]  B. Boyan,et al.  Local factor production by MG63 osteoblast-like cells in response to surface roughness and 1,25-(OH)2D3 is mediated via protein kinase C- and protein kinase A-dependent pathways. , 2001, Biomaterials.