The protective effect of vitamin C on Azoxymethane-induced oxidative stress in colon of mice.

Purpose: The Azoxymethane model for colon carcinogenesis is often used to study on the initiation and promotion stages of colon carcinogenesis in mice. Our aim in this study was to evaluate the effects of vitamin C on levels of oxidative stress parameters in azoxymethane induced colonic oxidative stress in mice. Material and Methods: In this purpose thirty Swiss albino mice (weighing 31.49±3.1 g), aging 12 weeks were randomly divided into three groups of ten each. Animals of group I and II were treated with weekly doses of subcutaneous 5 mg/kg azoxymethane for 7 weeks, whereas, the mice in group II were further treated with 500 mg/kg vitamin C administered intraperitoneally during the same period. Group III served as the control group. Results: In the vitamin C treated mice, levels of MDA and NO were significantly lower than those of azoxymethane only group (p=0.031, p<0.001). The levels of GSH in the vitamin C treated mice were significantly higher than that of azoxymethane only group (p=0.004). Conclusion: The present study demonstrates that intraperitoneal administration of vitamin C reduces azoxymethane induced oxidative stress in colon of mice.

[1]  C. Rao.  Nitric oxide signaling in colon cancer chemoprevention. , 2004, Mutation research.

[2]  J. Lai,et al.  Oxidation of vitamin E and vitamin C and inhibition of brain mitochondrial oxidative phosphorylation by peroxynitrite , 2004, Journal of neuroscience research.

[3]  A. Laudanna,et al.  Protective effect of ascorbic acid in experimental gastric cancer: reduction of oxidative stress. , 2003, World journal of gastroenterology.

[4]  M. Wellman,et al.  Nitric Oxide Exposure of CC531 Rat Colon Carcinoma Cells Induces γ-glutamyltransferase which May Counteract Glutathione Depletion and Cell Death , 2003, Free radical research.

[5]  L. Marnett.  Oxy radicals, lipid peroxidation and DNA damage. , 2002, Toxicology.

[6]  M. Bissonnette,et al.  Ursodeoxycholic acid inhibits the initiation and postinitiation phases of azoxymethane-induced colonic tumor development. , 2002, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.

[7]  H. Bartsch,et al.  Potential role of lipid peroxidation derived DNA damage in human colon carcinogenesis: studies on exocyclic base adducts as stable oxidative stress markers. , 2002, Cancer detection and prevention.

[8]  Jianwei Zhu,et al.  Upregulation of vascular endothelial growth factor by hydrogen peroxide in human colon cancer. , 2002, World journal of gastroenterology.

[9]  Hiromitsu Watanabe,et al.  Supplemental Silk Protein, Sericin, Suppresses Colon Tumorigenesis in 1,2-Dimethylhydrazine-Treated Mice by Reducing Oxidative Stress and Cell Proliferation , 2001, Bioscience, biotechnology, and biochemistry.

[10]  L. Brady,et al.  The role of probiotic cultures in the prevention of colon cancer. , 2000, The Journal of nutrition.

[11]  G. Poppel,et al.  Vitamins and cancer , 1997 .

[12]  P. Cerutti.  Oxy-radicals and cancer , 1994, The Lancet.

[13]  P. Cerutti,et al.  Oxidant stress and carcinogenesis , 1991, European journal of clinical investigation.

[14]  N. Cortas,et al.  Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. , 1990, Clinical chemistry.

[15]  J. Hankin,et al.  Diet and colorectal cancer with special reference to fiber intake , 1989, International journal of cancer.

[16]  M. Uchiyama,et al.  Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. , 1978, Analytical biochemistry.

[17]  R. Bostick.  Diet and Nutrition in the Etiology and Primary Prevention of Colon Cancer , 2001 .

[18]  H. Bernstein,et al.  Role of nitric oxide and peroxynitrite in bile salt-induced apoptosis: relevance to colon carcinogenesis. , 1999, Nutrition and cancer.

[19]  G. Block,et al.  Vitamin C and cancer prevention: the epidemiologic evidence. , 1991, The American journal of clinical nutrition.

[20]  S. Tannenbaum,et al.  Inhibition of nitrosamine formation by ascorbic acid. , 1991, The American journal of clinical nutrition.

[21]  S. Winawer,et al.  Primary prevention of colorectal cancer. The WHO Collaborating Centre for the Prevention of Colorectal Cancer. , 1990, Bulletin of the World Health Organization.

[22]  E. Beutler.  Glutathione in red blood cell metabolism , 1975 .