Forisomes: calcium-powered protein complexes with potential as 'smart' biomaterials.

Sieve tubes in legumes contain forisomes, which are spindle-like bodies that are composed of ATP-independent, mechanically active proteins. Upon injury, forisomes occlude sieve tubes by dispersion and thus, help to prevent loss of nutrient-rich transport sap. Forisome enlargement by dispersion is brought about by Ca2+-induced conformational changes that confer radial expansion and longitudinal contraction. Forisomes recontract upon Ca2+ removal. In vitro, forisomes reversibly disperse and contract in the presence or absence of Ca2+, respectively, and at distinct pHs. Recently, forisomes have received renewed attention because of their unique capacity to convert chemical into mechanical energy independent of high-energy organic compounds. Forisome-based 'smart' materials can be used to produce self-powered monitoring and diagnostic systems. Here, we focus on physiological, chemical and physical aspects of forisomes and discuss their potential as biomimetic devices.

[1]  P. Giavalisco,et al.  Towards the proteome of Brassica napus phloem sap , 2006, Proteomics.

[2]  Rahmat A. Shoureshi,et al.  Design of a biomimetic-based monitoring and diagnostic system for civil structures , 2007 .

[3]  R. Twyman,et al.  The Promoters of Forisome Genes MtSEO2 and MtSEO3 Direct Gene Expression to Immature Sieve Elements in Medicago truncatula and Nicotiana tabacum , 2009, Plant Molecular Biology Reporter.

[4]  P. Calvert,et al.  Multilayer Hydrogels as Muscle‐Like Actuators , 2000 .

[5]  H. Clausen‐Schaumann,et al.  The structure and functionality of contractile forisome protein aggregates. , 2008, Biomaterials.

[6]  N. Tuteja,et al.  Pea DNA helicase 45 overexpression in tobacco confers high salinity tolerance without affecting yield. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[7]  N. Tuteja,et al.  Cold, salinity and drought stresses: an overview. , 2005, Archives of biochemistry and biophysics.

[8]  M. Fricker,et al.  Forisome dispersion in Vicia faba is triggered by Ca2+ hotspots created by concerted action of diverse Ca2+ channels in sieve element , 2009, Plant signaling & behavior.

[9]  A. Bel,et al.  Microelectrode-recorded development of the symplasmic autonomy of the sieve element/companion cell complex in the stem phloem of Lupinus luteus L. , 2004, Planta.

[10]  N. Tuteja,et al.  Calcium- and salt-stress signaling in plants: shedding light on SOS pathway. , 2008, Archives of biochemistry and biophysics.

[11]  A. Furch,et al.  Plant- and stimulus-specific variations in remote-controlled sieve-tube occlusion , 2008, Plant signaling & behavior.

[12]  M. Shao,et al.  Bioengineering plant resistance to abiotic stresses by the global calcium signal system. , 2008, Biotechnology advances.

[13]  L. Mur,et al.  Nitric oxide generation in Vicia faba phloem cells reveals them to be sensitive detectors as well as possible systemic transducers of stress signals. , 2008, The New phytologist.

[14]  D. B. Fisher.  Structure of functional soybean sieve elements. , 1975, Plant physiology.

[15]  P. Giavalisco,et al.  Proteomics of curcurbit phloem exudate reveals a network of defence proteins. , 2004, Phytochemistry.

[16]  W. Pickard,et al.  Elastic properties of the forisome. , 2007, Functional plant biology : FPB.

[17]  H. Behnke.  SIEVE‐TUBE PLASTIDS OF MAGNOLIIDAE AND RANUNCULIDAE IN RELATION TO SYSTEMATICS , 1971 .

[18]  H. Behnke.  Nondispersive Protein Bodies in Sieve Elements: A Survey and Review of their Origin, Distribution and Taxonomic Significance , 1991 .

[19]  Cwm Yuen,et al.  SELECTED APPLICATIONS OF NANOTECHNOLOGY IN TEXTILES , 2006 .

[20]  C. Duschl,et al.  A Biohybrid Microfluidic Valve Based on Forisome Protein Complexes , 2008, Journal of Microelectromechanical Systems.

[21]  H. Behnke,et al.  Sieve Elements: Comparative Structure, Induction, and Development , 1990 .

[22]  Signaling in plants. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[23]  A. V. van Bel,et al.  The geometry of the forisome-sieve element-sieve plate complex in the phloem of Vicia faba L. leaflets. , 2006, Journal of experimental botany.

[24]  A. Bel,et al.  The phloem, a miracle of ingenuity , 2003 .

[25]  Rainer Stahlberg,et al.  The phytomimetic potential of three types of hydration motors that drive nastic plant movements , 2009 .

[26]  A. Furch,et al.  Functional Sieve Element Protoplasts1[OA] , 2007, Plant Physiology.

[27]  Nobutaka Hirokawa,et al.  Analysis of the kinesin superfamily: insights into structure and function. , 2005, Trends in cell biology.

[28]  P. Calvert.  Hydrogels for Soft Machines , 2009 .

[29]  N. Tuteja,et al.  Chemical signaling under abiotic stress environment in plants , 2008, Plant signaling & behavior.

[30]  A. V. van Bel,et al.  GFP Tagging of Sieve Element Occlusion (SEO) Proteins Results in Green Fluorescent Forisomes , 2008, Plant & cell physiology.

[31]  Henrik Bruus,et al.  Osmotically driven flows in microchannels separated by a semipermeable membrane. , 2008, Lab on a chip.

[32]  N. Tuteja,et al.  DNA helicases: the long unwinding road , 1996, Nature Genetics.

[33]  R. Fischer,et al.  Spatial and temporal regulation of the forisome gene for1 in the phloem during plant development , 2007, Plant Molecular Biology.

[34]  Constantinos Mavroidis,et al.  From pulses to motors , 2003, Nature materials.

[35]  R. Leurs,et al.  The effect of mutations in the DRY motif on the constitutive activity and structural instability of the histamine H(2) receptor. , 2000, Molecular pharmacology.

[36]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[37]  T. Müller,et al.  ATP-independent contractile proteins from plants , 2003, Nature materials.

[38]  H. Behnke.  Distribution and Evolution of Forms and Types of Sieve-element Plastids in the Dicotyledons , 1991 .

[39]  Winfried S Peters,et al.  Forisomes, a novel type of Ca(2+)-dependent contractile protein motor. , 2004, Cell motility and the cytoskeleton.

[40]  M. Fricker,et al.  Sieve Element Ca2+ Channels as Relay Stations between Remote Stimuli and Sieve Tube Occlusion in Vicia faba[W] , 2009, The Plant Cell Online.

[41]  Amy Q. Shen,et al.  Forisome as biomimetic smart materials , 2005, SPIE Smart Structures and Materials + Nondestructive Evaluation and Health Monitoring.

[42]  W. Wergin,et al.  Formation and dispersal of crystalline P-protein in sieve elements of soybean (Glycine max L.) , 1970, Protoplasma.

[43]  N. Tuteja.  Unraveling DNA helicases from plant cells* , 1997, Plant Molecular Biology.

[44]  A. V. van Bel,et al.  Reversible Calcium-Regulated Stopcocks in Legume Sieve Tubes , 2001, Plant Cell.

[45]  S. Schwan,et al.  Micromechanical measurements on P-protein aggregates (forisomes) from Vicia faba plants. , 2009, Biophysical chemistry.

[46]  P. Ashton,et al.  The secreted salivary proteome of the pea aphid Acyrthosiphon pisum characterised by mass spectrometry , 2009, Proteomics.

[47]  A. Bel,et al.  Ultrastructural features of well-preserved and injured sieve elements: Minute clamps keep the phloem transport conduits free for mass flow , 2000, Protoplasma.

[48]  Michael J E Sternberg,et al.  Exploring the extremes of sequence/structure space with ensemble fold recognition in the program Phyre , 2008, Proteins.

[49]  A. Bel,et al.  Microelectrode-recorded development of the symplasmic autonomy of the sieve element/companion cell complex in the stem phloem ofLupinus luteus L. , 2004, Planta.

[50]  E. Newcomb,et al.  The ultrastructure and development of tubular and crystalline P-protein in the sieve elements of certain papilionaceous legumes , 1971, Protoplasma.

[51]  Narendra Tuteja,et al.  Mechanisms of high salinity tolerance in plants. , 2007, Methods in enzymology.

[52]  M. Nei,et al.  MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. , 2007, Molecular biology and evolution.

[53]  W. F. Tjallingii,et al.  Molecular sabotage of plant defense by aphid saliva , 2007, Proceedings of the National Academy of Sciences.

[54]  M. Knoblauch,et al.  Biomimetic actuators: where technology and cell biology merge , 2004, Cellular and Molecular Life Sciences CMLS.

[55]  J. Kehr,et al.  Evidence for the presence and activity of a complete antioxidant defence system in mature sieve tubes. , 2002, The Plant journal : for cell and molecular biology.

[56]  S. Schwan,et al.  In vitro investigation of the geometric contraction behavior of chemo-mechanical P-protein aggregates (forisomes). , 2007, Biophysical chemistry.

[57]  C. Constantinidou,et al.  Determining protein identity from sieve element sap in Ricinus communis L. by quadrupole time of flight (Q-TOF) mass spectrometry. , 2004, Journal of experimental botany.

[58]  M. Knoblauch,et al.  Research note: Reversible birefringence suggests a role for molecular self-assembly in forisome contractility. , 2007, Functional plant biology : FPB.

[59]  N. Tuteja.  Integrated Calcium Signaling in Plants , 2009 .

[60]  N. Tuteja,et al.  Helicases as molecular motors: An insight , 2006, Physica A: Statistical Mechanics and its Applications.

[61]  N. Tuteja,et al.  Cold- and salinity stress-induced bipolar pea DNA helicase 47 is involved in protein synthesis and stimulated by phosphorylation with protein kinase C. , 2005, The Plant journal : for cell and molecular biology.

[62]  W. Pickard,et al.  Tailed forisomes of Canavalia gladiata: a new model to study Ca2+-driven protein contractility. , 2007, Annals of botany.

[63]  B. Ranjbar,et al.  Nanotechnology helps medicine: nanoscale swimmers and their future applications. , 2005, Medical hypotheses.

[64]  Measurement of mechanical forces generated by plant P-protein aggregates (forisomes) , 2009, European Biophysics Journal.

[65]  Amy Q Shen,et al.  Anisotropic contraction in forisomes: simple models won't fit. , 2008, Cell motility and the cytoskeleton.

[66]  A. V. van Bel,et al.  Sieve Tubes in Action , 1998, Plant Cell.

[67]  H. Kleinig,et al.  Protein filaments-structural components of the phloem exudate , 1970, Planta.

[68]  R. Shetty.  Potential pitfalls of nanotechnology in its applications to medicine: immune incompatibility of nanodevices. , 2005, Medical hypotheses.

[69]  A. Schulz,et al.  Journal of Experimental Botany Advance Access published July 19, 2007 Journal of Experimental Botany, Page 1 of 12 , 2022 .

[70]  Joost T. van Dongen,et al.  Phloem Metabolism and Function Have to Cope with Low Internal Oxygen1 , 2003, Plant Physiology.

[71]  R. Park,et al.  The regulation of gelation of Phloem exudate from cucurbita fruit by dilution, glutathione, and glutathione reductase. , 1988, Plant physiology.

[72]  Winfried S. Peters,et al.  Erratum: Corrigendum: ATP-independent contractile proteins from plants , 2005 .

[73]  S. Yanagisawa,et al.  Nano scale proteomics revealed the presence of regulatory proteins including three FT-Like proteins in phloem and xylem saps from rice. , 2008, Plant & cell physiology.