Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation.
暂无分享,去创建一个
T. C. Evans | Jack Benner | S. Chong | M. Q. Xu | I. Chute | X. Liu | H. Wu | S. Mathys | M. Q. Xu
[1] T. C. Evans,et al. The in Vitro Ligation of Bacterially Expressed Proteins Using an Intein from Methanobacterium thermoautotrophicum * , 1999, The Journal of Biological Chemistry.
[2] Jack Benner,et al. Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. , 1998, Nucleic acids research.
[3] T. C. Evans,et al. Semisynthesis of cytotoxic proteins using a modified protein splicing element , 1998, Protein science : a publication of the Protein Society.
[4] F. S. Gimble. Putting protein splicing to work. , 1998, Chemistry & biology.
[5] H. Wu,et al. Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein. , 1998, Biochimica et biophysica acta.
[6] T. Muir,et al. Expressed Protein Ligation, a Novel Method for Studying Protein-Protein Interactions in Transcription* , 1998, The Journal of Biological Chemistry.
[7] T. Muir,et al. Expressed protein ligation: a general method for protein engineering. , 1998, Proceedings of the National Academy of Sciences of the United States of America.
[8] Ming-Qun Xu,et al. Modulation of Protein Splicing of the Saccharomyces cerevisiae Vacuolar Membrane ATPase Intein* , 1998, The Journal of Biological Chemistry.
[9] S. Pietrokovski,et al. Modular organization of inteins and C‐terminal autocatalytic domains , 1998, Protein science : a publication of the Protein Society.
[10] Amalio Telenti,et al. Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing , 1998, Nature Structural Biology.
[11] H. Paulus,et al. Protein splicing: estimation of the rate of O‐N and S‐N acyl rearrangements, the last step of the splicing process , 2009 .
[12] F. Perler,et al. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. , 1997, Gene.
[13] G J Olsen,et al. Compilation and analysis of intein sequences. , 1997, Nucleic acids research.
[14] F. Perler,et al. The mechanism of protein splicing and its modulation by mutation. , 1996, The EMBO journal.
[15] F. Perler,et al. Protein Splicing Involving the Saccharomyces cerevisiae VMA Intein , 1996, The Journal of Biological Chemistry.
[16] S. Pietrokovski,et al. A new intein in cyanobacteria and its significance for the spread of inteins. , 1996, Trends in genetics : TIG.
[17] H. Paulus,et al. Protein splicing: evidence for an N-O acyl rearrangement as the initial step in the splicing process. , 1996, Biochemistry.
[18] J. Tam,et al. Peptide synthesis using unprotected peptides through orthogonal coupling methods. , 1995, Proceedings of the National Academy of Sciences of the United States of America.
[19] J. Mccoy,et al. Gene fusion expression systems in Escherichia coli. , 1995, Current opinion in biotechnology.
[20] F. Perler,et al. Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation. , 1994, The EMBO journal.
[21] M. Hashimoto,et al. The roles of the C-terminal domain and type III domains of chitinase A1 from Bacillus circulans WL-12 in chitin degradation , 1994, Journal of bacteriology.
[22] N. Neff,et al. Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature. , 1994, Nucleic acids research.
[23] T. Muir,et al. Synthesis of proteins by native chemical ligation. , 1994, Science.
[24] Francine B. Perler,et al. In vitro protein splicing of purified precursor and the identification of a branched intermediate , 1993, Cell.
[25] T. Stevens,et al. Protein splicing of the yeast TFP1 intervening protein sequence: a model for self‐excision. , 1993, The EMBO journal.
[26] S. Sedgwick,et al. Protein splicing in the maturation of M. tuberculosis RecA protein: A mechanism for tolerating a novel class of intervening sequence , 1992, Cell.
[27] F. Studier,et al. Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. , 1991, Journal of molecular biology.
[28] P. Kane,et al. Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase. , 1990, Science.
[29] R. Hirata,et al. Molecular structure of a gene, VMA1, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. , 1990, The Journal of biological chemistry.
[30] F. Studier,et al. Use of T7 RNA polymerase to direct expression of cloned genes. , 1990, Methods in enzymology.
[31] Chu di Guana,et al. Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. , 1988 .
[32] D. Smith,et al. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. , 1988, Gene.
[33] Thomas A. Kunkel,et al. Rapid and efficient site-specific mutagenesis without phenotypic selection. , 1985, Proceedings of the National Academy of Sciences of the United States of America.
[34] A. Tsugita,et al. Primary structure and genetic organization of phage T4 DNA ligase. , 1983, Nucleic acids research.
[35] M. M. Bradford. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.