Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation.

[1]  T. C. Evans,et al.  The in Vitro Ligation of Bacterially Expressed Proteins Using an Intein from Methanobacterium thermoautotrophicum * , 1999, The Journal of Biological Chemistry.

[2]  Jack Benner,et al.  Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. , 1998, Nucleic acids research.

[3]  T. C. Evans,et al.  Semisynthesis of cytotoxic proteins using a modified protein splicing element , 1998, Protein science : a publication of the Protein Society.

[4]  F. S. Gimble Putting protein splicing to work. , 1998, Chemistry & biology.

[5]  H. Wu,et al.  Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein. , 1998, Biochimica et biophysica acta.

[6]  T. Muir,et al.  Expressed Protein Ligation, a Novel Method for Studying Protein-Protein Interactions in Transcription* , 1998, The Journal of Biological Chemistry.

[7]  T. Muir,et al.  Expressed protein ligation: a general method for protein engineering. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[8]  Ming-Qun Xu,et al.  Modulation of Protein Splicing of the Saccharomyces cerevisiae Vacuolar Membrane ATPase Intein* , 1998, The Journal of Biological Chemistry.

[9]  S. Pietrokovski,et al.  Modular organization of inteins and C‐terminal autocatalytic domains , 1998, Protein science : a publication of the Protein Society.

[10]  Amalio Telenti,et al.  Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing , 1998, Nature Structural Biology.

[11]  H. Paulus,et al.  Protein splicing: estimation of the rate of O‐N and S‐N acyl rearrangements, the last step of the splicing process , 2009 .

[12]  F. Perler,et al.  Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. , 1997, Gene.

[13]  G J Olsen,et al.  Compilation and analysis of intein sequences. , 1997, Nucleic acids research.

[14]  F. Perler,et al.  The mechanism of protein splicing and its modulation by mutation. , 1996, The EMBO journal.

[15]  F. Perler,et al.  Protein Splicing Involving the Saccharomyces cerevisiae VMA Intein , 1996, The Journal of Biological Chemistry.

[16]  S. Pietrokovski,et al.  A new intein in cyanobacteria and its significance for the spread of inteins. , 1996, Trends in genetics : TIG.

[17]  H. Paulus,et al.  Protein splicing: evidence for an N-O acyl rearrangement as the initial step in the splicing process. , 1996, Biochemistry.

[18]  J. Tam,et al.  Peptide synthesis using unprotected peptides through orthogonal coupling methods. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[19]  J. Mccoy,et al.  Gene fusion expression systems in Escherichia coli. , 1995, Current opinion in biotechnology.

[20]  F. Perler,et al.  Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation. , 1994, The EMBO journal.

[21]  M. Hashimoto,et al.  The roles of the C-terminal domain and type III domains of chitinase A1 from Bacillus circulans WL-12 in chitin degradation , 1994, Journal of bacteriology.

[22]  N. Neff,et al.  Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature. , 1994, Nucleic acids research.

[23]  T. Muir,et al.  Synthesis of proteins by native chemical ligation. , 1994, Science.

[24]  Francine B. Perler,et al.  In vitro protein splicing of purified precursor and the identification of a branched intermediate , 1993, Cell.

[25]  T. Stevens,et al.  Protein splicing of the yeast TFP1 intervening protein sequence: a model for self‐excision. , 1993, The EMBO journal.

[26]  S. Sedgwick,et al.  Protein splicing in the maturation of M. tuberculosis RecA protein: A mechanism for tolerating a novel class of intervening sequence , 1992, Cell.

[27]  F. Studier,et al.  Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. , 1991, Journal of molecular biology.

[28]  P. Kane,et al.  Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase. , 1990, Science.

[29]  R. Hirata,et al.  Molecular structure of a gene, VMA1, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. , 1990, The Journal of biological chemistry.

[30]  F. Studier,et al.  Use of T7 RNA polymerase to direct expression of cloned genes. , 1990, Methods in enzymology.

[31]  Chu di Guana,et al.  Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. , 1988 .

[32]  D. Smith,et al.  Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. , 1988, Gene.

[33]  Thomas A. Kunkel,et al.  Rapid and efficient site-specific mutagenesis without phenotypic selection. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[34]  A. Tsugita,et al.  Primary structure and genetic organization of phage T4 DNA ligase. , 1983, Nucleic acids research.

[35]  M. M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.